Decolorization and Metabolism of Anthraquionone-Type Dye by Laccase of White-Rot Fungi Polyporus sp. S133

Abstract

Utilization of microbes including white-rot fungi and bacterial strains for decolorization of synthetic dyes is one promising strategy of an environmentally friendly and cost-competitive alternative to physicochemical decomposition processes for treating industrial effluents. In this study, the biodegradation ability of the white-rot fungi Polyporus sp. S133 that produce high laccase was investigated in order to decolorize anthraquinone-type dye. Parameter including pH, temperature, and non-ionic surfactant were used to comparatively study the decolorizing effects on Remazol Brilliant Blue R (RBBR). The purified laccase totally decolorized 200 mg L −1 initial concentration of RBBR dye when only 1.5 UL −1 of laccase was used in the reaction mixture. The optimal decolorization rates were achieved at pH 5 and at a temperature of 50°C. N-hydroxybenzotriazole, a small molecular weight redox mediator, was found to accelerate the decolorization. Tween 20 inhibited the decolorization while Tween 80 and Brij 35 showed no inhibition effect. Two compounds were identified as the intermediates (m/z 304.3 and m/z 342.2). These results suggest that laccase from Polyporus sp. S133 is a powerful tool for the decolorization of anthraquinone dyes. A pathway for the metabolism of the RBBR by laccase of Polyporus sp S133 was proposed. These proposed pathways could contribute to a better comprehension of the mechanisms used by oxidative enzymes to transform organic compounds.

Evaluation of Catalytic Efficiency of Coriolopsis caperata DN Laccase to Decolorize and Detoxify RBBR Dye

“…The present study proposed that laccase oxidizes RBBR dye and produced two subproducts (sodium1-amino-9,10-dioxo-9,10-dihydroanthracene-2-sulphonate and sodium 2-((3-aminophenyl) sulfonyl) ethyl sulfate) (Fig. 6), which is similar to the result reported by Osma et al [24] and Hadibarata et al [2].…”

“…The mobile phase was composed of acetonitrile and distilled water (65:35) while the temperature of column oven was 280°C and of injection was 200°C. Temperature was hold at 40°C for 1 min then rose to 280°C at 10°C rise min −1 [2]. …”

“…Every year, at least 10% of the used dyestuff enters in the environment out of 800,000 tons of world production [1]. Anthraquinone dye Remazol Brilliant Blue R (RBBR) is frequently used for the production of polymeric dyes but has toxicity and recalcitrance due to the anthracene derivative [2] and is possibly carcinogenic to humans (group 2B) (https:// monographs.iarc.fr/ENG/Monographs/vol101/mono101-001). Presence of dyes in water can cause diseases like hemorrhage, ulceration of skin, nausea, severe irritation of skin, and dermatitis.…”

Cloning and characterization of CotA laccase from Bacillus subtilis WD23 decoloring dyes

“…Anthraquinone dye was the laccase substrate, which was similar to previous reports for Polyporus sp. S133 (Hadibarata et al 2012a), Armillaria sp. F022 (Hadibarata et al 2012b), Trametes sp.…”

Lenzites elegans KSG32: A Novel White Rot Fungus for Synthetic Dye Decolourization

“…Based on the reports of [36] and [47] the efficiency of decolorization by laccase increases in the presence of LiP, which helps to overcome the substrate unspecificity of laccase. In the present study, L. elegans KSG32 produce both laccase and LiP which causes more significant decolorization.…”