Decolorization and degradation potential of enhanced lignocellulolytic enzymes production by Pleurotus eryngii using cherry waste from industry

Abstract

Lignocellulosic wastes accumulate in large quantities and thus cause environmental issues. Cherry waste (CW) of them collected from industry was used as the substrate to increase production of lignocellulolytic enzymes, laccase (Lac), manganese peroxidase (MnP), lignin peroxidase (LiP), carboxymethyl cellulase (CmCase), xylanase, exoglucanase, β-glucosidase (BGLA), by Pleurotus eryngii. Then, the decolorizations of some azo dyes were examined. The effects of different concentrations of some compounds, such as copper, iron, Tween 80, ammonium nitrate, and manganese, on the productions of lignocellulolytic enzymes were studied depending on incubation period. The maximum productions of lignocellulolytic enzymes were achieved by performing 5.0 g CW and 1,000 µM Cu 2+ , 1,000 µM Fe 2+ , 2.0 g L −1 ammonium nitrate, 180 µM Mn 2+ as the inducers. To the results determined under optimized conditions, 3.61, 4.79, 1.86, 1.15, 2.24, and 2.91-fold increases were respectively obtained for Lac, MnP, LiP, CMCase, xylanase, and BGLA activities. The chemical changes of dye structure during decolorization by lignocellulolytic enzymes extract containing Lac with decolorization performance as 12.6 ± 0.8% were partially characterized using Fourier transform infrared spectroscopy. This study is important in terms of dye decolorization and degradation by achieving the enhancement of the activities of seven lignocellulolytic enzymes using various inductors.

Solid-state fermentation on poplar sawdust and corncob wastes for lignocellulolytic enzymes by different Pleurotus ostreatus strains

“…Many factors affect the secretion of lignocellulolytic enzyme production by fungi, such as the fermentation method, the type and complexity of carbon and nitrogen sources, ion concentration, temperature, and more (Stajić et al 2006;Sohail et al 2009;Zhang et al 2013;Bentil et al 2018;Ferraz et al 2019;Filipe et al 2019;Akpinar and Urek 2020;Rajavat et al 2020). The types of substrates and the mode of cultivation of fungal species play an essential role in enzyme productivity.…”

Assessment of anammox, microalgae and white-rot fungi-based processes for the treatment of textile wastewater

“…Results showed that colour removal was positively correlated with the presence of Iron in the textile wastewater samples (ρ = 0.77, p-value = 0.041). Iron, together with Copper, have been already reported to induce the enzymatic activities correlated to colour removal of cherry waste by in Pleurotus eringii [63].…”

Biofuels Production from Renewable Resources

“…The review envisions the improvement of the activity of DyPs against lignin to reach satisfactory conversion levels for industrial applications. In line with the requirements for sustainable scale‐up, Akpinar and Urek [7] used cherry waste collected from industry as substrate and Pleurotus eryngii as the microorganism to increase production of lignocellulolytic enzymes such as laccase (Lac), manganese peroxidase, lignin peroxidase, carboxymethyl cellulase, xylanase, exoglucanase, and β‐glucosidase. The data show that P. eryngii adapted to all solid‐state fermentation conditions and resulted in increased enzymatic activities that also demonstrated decolorizing performance.…”