Decolorization and biodegradation of Reactive Blue 13 by Proteus mirabilis LAG

Olukanni, Olumide D.; Osuntoki, Akinniyi Adediran; Kalyani, Dayanand; Gbenle, George O.; Govindwar, Sanjay P.

doi:10.1016/j.jhazmat.2010.08.035

Cited by 103 publications

(41 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These observations were completely different from those in Figure 7c (Orange G control) with two peaks at 3.1 min and 3.9 min and absorbance beyond 500 nm. Similar results were presented during a HPLC follow-up of dye biodegradation [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33]. The FTIR spectrum as well as the chromatogram of control (fungi alone) (not presented) were different from those of the degradation products (Figure 6b, 6d, 7b and 7d).…”

Section: Analysis Of Degradation Products By Ftir and Hplcsupporting

confidence: 76%

“…). Different authors have also shown similar changes during the biodegradation of certain azo and anthraquinone dyes [14][15][16].…”

Section: Analysis Of Degradation Products By Ftir and Hplcmentioning

confidence: 86%

“…Studies have shown that dyes such as the RB4 and Orange G can be degraded by fungi such as T. versicolor, P. sordida, T. lauteus and P. flabellatus [5,13]. In addition, using analytical methods such as FTIR, UV-Vis and HPLC authors have monitoring the decolorization and microbial degradation [14][15][16]. The present study reports the ability of the P. sanguineus MUCL 51321 fungus to decolour and degrade two recalcitrant synthetic dyes (RB4 and Orange G).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Attéké

Mounguengui²,

Jean-Bosco

et al. 2013

J Bioremed Biodeg

View full text Add to dashboard Cite

The fungus Pycnoporus sanguineus MUCL 51321 white rot isolated in Gabon showed a high ability to decolorize and degrade two synthetic dyes. On solid and liquid media, the fungus had different enzyme activities (laccase and manganese (Mn) peroxidase) in the presence of different substrates. At concentrations of 0.05 g/L (Orange G) and 0.3 g/L (reactive blue 4), the respective rates of decolorization of 81% and 97% were observed after 15 days of incubation in liquid media. In the same time interval, changes on the spectra (UV-vis and FTIR) and chromatograms (HPLC) showed that these decolorizations were due to the degradation of dyes by fungus with the production of new compounds. The study revealed the possibility of the use of this fungal strain in the microbial degradation process of synthetic dyes. Aldrich (Sigma-Aldrich, St. Louis, MO, USA). All reagents used were of the analytical grade. Journal of Bior emediation & Biodegradation Culture ConditionsThe composition of the solid growth medium used was as follows: 0.2% glucose, 0.2% malt extract, 0.01% MgSO 4 , 0.01% MnCl 2 , 0.03% NH 4 NO 3 , 0.026% KH 2 PO 4 , 0.026% Na 2 HPO 4 , 0.01% CaCl 2 .2H 2 O, 0.0001% FeC1 3 .6H 2 O, 0.0001% ZnCl 2 , 1.6% and 1.6% agar in 1 L of distilled water. The pH was adjusted to 5.5 with hydrochloric acid (0. 5 N) before autoclaving (at 121°C for 15 min). The culture medium was transferred into 4 Erlenmeyer flasks (200 mL each) and each reagent (enzymatic revelation) or dye, depending on its final concentration was added. The mixture was shaken, then about 15 mL were distributed in a Petri dish (diameter 8.5 mm); each test was replicated three times. 50 mL of liquid culture media in flasks of 100 mL were constituted in almost the same way as the solid culture media described above, except for the absence of agar. Each test was conducted three times. Decolorization experimentsDyes decolorization on solid medium: Solid culture media contained different concentrations of each dye (0.05 g/L to 1.5 g/L). Various controls were also prepared: some Petri dishes with fungus alone and others with dye alone to assess possible abiotic decolorization. One square portion of agar (1 cm of diameter) containing the fungus was placed at the centre of each Petri dish. The fungal growth diameters and the halos of decolorization were measured every day during one week at constants temperature (30°C) and humidity (75%). After these measures, different ratios and rates (decolorization or inhibition) were determined as described by . The optimum temperature of decolorization was evaluated using different growth temperatures (15, 22, 30, 35, 40 and 45°C). Moreover, the optimal concentrations for decolorization (0.05 g/L for the Orange G and 0.3 g/L for the RB4) were obtained after one week. All tests were conducted three times (averages are presented with the corresponding standard deviations).Dyes decolorization on liquid culture medium: For each Erlenmeyer flask (100 mL) containing liquid culture medium (50 mL) and dye (0.05 g/L Orange G or 0.3...

show abstract

Section: Analysis Of Degradation Products By Ftir and Hplcsupporting

confidence: 76%

“…). Different authors have also shown similar changes during the biodegradation of certain azo and anthraquinone dyes [14][15][16].…”

Section: Analysis Of Degradation Products By Ftir and Hplcmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Attéké

Mounguengui²,

Jean-Bosco

et al. 2013

J Bioremed Biodeg

View full text Add to dashboard Cite

show abstract

“…The activities of similar enzymes have been observed to be induced in the roots of Brassica juncea during the degradation of synthetic mixture of dyes and even textile effluents (Ghodake et al 2009). Inductions in the activities of all these enzymes during dye degradation have been reported in a number of bacteria (Olukanni et al 2010). Plants and microorganisms, including bacteria and fungi, contain many similar enzymes for detoxification or transformation of xenobiotics (Arthur et al 2005).…”

Section: Enzymatic Analysismentioning

confidence: 99%

Synergistic degradation of diazo dye Direct Red 5B by Portulaca grandiflora and Pseudomonas putida

Khandare

Kabra

Awate

et al. 2013

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

Plants and bacterial consortium of Portulaca grandiflora and Pseudomonas putida showed complete decolorization of a sulfonated diazo dye Direct Red 5B within 72 h, while in vitro cultures of P. grandiflora and P. putida independently showed 92 and 81 % decolorization within 96 h, respectively. A significant induction in the activities of lignin peroxidase, tyrosinase, 2,6-dichlorophenol indophenol reductase and riboflavin reductase was observed in the roots of P. grandiflora during dye decolorization; whereas, the activities of laccase, veratryl alcohol oxidase and 2,6-dichlorophenol indophenol reductase were induced in the cells of P. putida. Plant and bacterial enzymes in the consortium gave an enhanced decolorization of Direct Red 5B synergistically. The metabolites formed after dye degradation analyzed by UV-Vis spectroscopy, Fourier transformed infrared spectroscopy and high performance liquid chromatography confirmed the biotransformation of Direct Red 5B. Differential fate of metabolism of Direct Red 5B by P. grandiflora, P. putida and their consortium were proposed with the help of gas chromatography-mass spectroscopy analysis. P. grandiflora metabolized the dye to give 1-(4-diazenylphenyl)-2-phenyldiazene, 7-(benzylamino) naphthalene-2-sulfonic acid, 7-aminonaphthalene-2-sulfonic acid and methylbenzene. P. putida gave 4-hydroxybenzenesulfonic acid and 4-hydroxynaphthalene-2-sulfonic acid and benzamide. Consortium showed the formation of benzenesulfonic acid, 4-diazenylphenol, 6-aminonaphthalen-1-ol, methylbenzene and naphthalen-1-ol. Consortium achieved an enhanced and efficient degradation of Direct Red 5B. Phytotoxicity study revealed the nontoxic nature of metabolites formed after parent dye degradation. Use of such combinatorial systems of plant and bacteria could prove to be an effective and efficient strategy for the removal of textile dyes from soil and waterways.

show abstract

“…However, the most significant functioning protein of azo dye decolorization seemed to be azoreductase. In Proteus genus, only P. mirabilis LAG showed the capability to decolorize the azo dyes via a NADPHdependent azoreductase (Olukanni et al 2010). At least two azoreductases and two laccases were systematically reported in public domain, i.e., azoreductase, laccase, NADH-DCIP reductase, riboflavin reductase, lignin peroxidase (Saratale et al 2011;Han et al 2012).…”

Section: Genomic and Enzymes Studies Of Proteusmentioning

confidence: 99%

Electron transport phenomena of electroactive bacteria in microbial fuel cells: a review of Proteus hauseri

Hsueh

Chen

2017

Bioresour. Bioprocess.

View full text Add to dashboard Cite

This review tended to decipher the expression of electron transfer capability (e.g., biofilm formation, electron shuttles, swarming motility, dye decolorization, bioelectricity generation) to microbial fuel cells (MFCs). As mixed culture were known to perform better than pure microbial cultures for optimal expression of electrochemically stable activities to pollutant degradation and bioenergy recycling, Proteus hauseri isolated as a "keystone species" to maintain such ecologically stable potential for power generation in MFCs was characterized. P. hauseri expressed outstanding performance of electron transfer (ET)-associated characteristics [e.g., reductive decolorization (RD) and bioelectricity generation (BG)] for electrochemically steered bioremediation even though it is not a nanowire-generating bacterium. This review tended to uncover taxonomic classification, genetic or genomic characteristics, enzymatic functions, and bioelectricity-generating capabilities of Proteus spp. with perspectives for electrochemical practicability. As a matter of fact, using MFCs as a tool to evaluate ET capabilities, dye decolorizer(s) could clearly express excellent performance of simultaneous bioelectricity generation and reductive decolorization (SBG and RD) due to feedback catalysis of residual decolorized metabolites (DMs) as electron shuttles (ESs). Moreover, the presence of reduced intermediates of nitroaromatics or DMs as ESs could synergistically augment efficiency of reductive decolorization and power generation. With swarming mobility, P. hauseri could own significant biofilm-forming capability to sustain ecologically stable consortia for RD and BG. This mini-review evidently provided lost episodes of great significance about bioenergysteered applications in myriads of fields (e.g., biodegradation, biorefinery, and electro-fermentation). Keywords

show abstract

Decolorization and biodegradation of Reactive Blue 13 by Proteus mirabilis LAG

Cited by 103 publications

References 35 publications

Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Synergistic degradation of diazo dye Direct Red 5B by Portulaca grandiflora and Pseudomonas putida

Electron transport phenomena of electroactive bacteria in microbial fuel cells: a review of Proteus hauseri

Contact Info

Product

Resources

About