Decline in fertility of mouse sperm with abnormal chromatin during epididymal passage as revealed by ICSI

Suganuma, Ryota; Yanagimachi, Ryuzo; Meistrich, Marvin L.

doi:10.1093/humrep/dei169

Cited by 175 publications

(109 citation statements)

References 28 publications

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“…To date, several studies have reported higher ICSI pregnancy rates using testicular vs. ejaculated spermatozoa (in most series, ejaculated spermatozoa were used in the initial ICSI cycles) in men with cryptozoospermia (Bendikson et al, 2008;Hauser et al, 2011;Ben-Ami et al, 2013). These clinical findings support experimental observations demonstrating that in animals with impaired spermatogenesis, post-testicular spermatozoa have poorer chromatin integrity than testicular spermatozoa (Suganuma et al, 2005). In contrast, other investigators have reported favorable ICSI outcomes with the use of ejaculated spermatozoa (fresh or frozen-thawed) in men with cryptozoospermia and/or comparable ICSI outcomes using testicular vs. ejaculated spermatozoa in these men (Koscinski et al, 2007;Amirjannati et al, 2012).…”

Section: Discussionsupporting

confidence: 57%

Sperm retrieval outcomes with microdissection testicular sperm extraction (micro-TESE) in men with cryptozoospermia

Alrabeeah

Wachter²,

Phillips³

et al. 2015

Andrology

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SUMMARYSeveral studies support of the use of testicular rather than ejaculated spermatozoa for intracytoplasmic sperm injection (ICSI) in couples with virtual azoospermia or cryptozoospermia, although this approach remains controversial. We sought to evaluate sperm retrieval outcomes with microdissection testicular sperm extraction (micro-TESE) in men with cryptozoospermia. We conducted a retrospective study of 24 consecutive micro-TESEs in men with cryptozoospermia. We also evaluated the outcomes of seven consecutive TESAs (testicular sperm aspiration) in cryptozoospermic men during the same time period (January 2007 and September 2014). Micro-TESE and TESA were performed on the day prior to ICSI. Final assessment of sperm recovery (reported on the day of ICSI) was recorded as (i) successful (available spermatozoa for ICSI) or (ii) unsuccessful (no spermatozoa for ICSI). The decision to perform a unilateral or bilateral micro-TESE was guided by the intra-operative evaluation of sperm recovery from the first testicle. A unilateral procedure was performed in 87.5% (21/24) and 57% (4/7) of the micro-TESE and TESA cohorts, respectively. Sperm recovery was successful in 96% (23/24) of the men who underwent micro-TESE and 43% (3/7) of the men who underwent TESA (p < 0.01). The ICSI pregnancy rates (per embryo transfer) in the micro-TESE and TESA groups were comparable [33% (6/18) and 50% (1/2), respectively]. The data indicate that micro-TESE is a highly successful sperm retrieval technique for men with cryptozoospermia and few of these men will require a bilateral procedure. Moreover, sperm retrieval rates are higher with micro-TESE than TESA in this group of men.

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Section: Discussionsupporting

confidence: 57%

Sperm retrieval outcomes with microdissection testicular sperm extraction (micro-TESE) in men with cryptozoospermia

Alrabeeah

Wachter²,

Phillips³

et al. 2015

Andrology

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“…The testes have substantial antioxidant systems, but once spermatozoa are released from the Sertoli cells and migrate from the seminiferous tubules to the epididymis, they become susceptible to oxidative stress [110]. DNA damage in testicular spermatozoa is threefold lower compared with ejaculated spermatozoa [111].…”

Section: Testicular Sperm Extractionmentioning

confidence: 99%

Reactive Oxygen Species and Sperm Cells

Takeshima¹,

Kuroda²,

Yumura³

2018

Reactive Oxygen Species (ROS) in Living Cells

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Many cases of male factor infertility are idiopathic, but 30-40% of cases may have excessive levels of reactive oxygen species (ROS) in their semen. The origins of endogenous ROS are leukocytes and immature spermatozoa, and external causes are various. On the contrary, seminal plasma contains various antioxidants. Low levels of ROS are essential for the fertilization process, but excessive levels of ROS lead to oxidative stress and can have harmful effects such as lipid peroxidation of a membrane, sperm deoxyribonucleic acid fragmentation, and apoptosis on the fertile capacity. In order to evaluate oxidative stress appropriately, ROS is measured by the chemiluminescence method with neat semen and quantification of 8-OH-2′-deoxyguanosine and malondialdehyde in seminal plasma. Antioxidant potential is often measured using total antioxidant capacity (TAC) assay. The oxidation-reduction potential measured by a MiOXSYS analyzer is a novel, easier, quicker, and less expensive technology to measure oxidative stress. In order to minimize oxidative stress and improve clinical outcomes, sperm-sorting methods, lifestyle modifications, shortening the ejaculatory abstinence, and treatments such as oral antioxidants, varicocelectomy, and testicular sperm extraction are taken into account. As a future prospect, proteomics, metabolomics, and genomics are still developing areas that have the potential to discover new findings and highly sensitive biomarkers.

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“…This protein can reduce H 2 O 2 and cellular hydroperoxides, and may play a role in oxidative injury [41]. There is now substantial evidence for oxidative injury in spermatozoa of infertile patients, which in turn leads to increased injury of the genome [42]. Another interesting protein that could have been included in this category is the product of PARK7, which we have included under the protein synthesis category (see above).…”

mentioning

confidence: 99%

Proteomic identification of human sperm proteins

Martínez-Heredia¹,

Estanyol²,

Ballescà³

et al. 2006

Proteomics

230

177

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Conventional 1-DE has in the past provided a wealth of information concerning the major sperm proteins. However, so far there are relatively few reports exploiting the potential of the present proteomic tools to identify and to study additional yet-unidentified important proteins present in human spermatozoa. In the present work, 2-DE of proteins extracted from human normozoospermic spermatozoa led to the resolution of over 1000 spots. Subsequent excision from the gels of 145 spots and MALDI-TOF MS analysis allowed the identification of 98 different proteins. The function of these proteins turned out to be energy production (23%), transcription, protein synthesis, transport, folding and turnover (23%), cell cycle, apoptosis and oxidative stress (10%), signal transduction (8%), cytoskeleton, flagella and cell movement (10%), cell recognition (7%), metabolism (6%) and unknown function (11%). As many as 23% of the proteins identified have not been previously described as being expressed in human spermatozoa. The present data provide an important clue towards determining the function of these proteins and opens up the possibility to perform additional experiments.

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Decline in fertility of mouse sperm with abnormal chromatin during epididymal passage as revealed by ICSI

Cited by 175 publications

References 28 publications

Sperm retrieval outcomes with microdissection testicular sperm extraction (micro-TESE) in men with cryptozoospermia

Sperm retrieval outcomes with microdissection testicular sperm extraction (micro-TESE) in men with cryptozoospermia

Reactive Oxygen Species and Sperm Cells

Proteomic identification of human sperm proteins

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