Lignin isolated from beech sawdust was used for the preparation
of lignin nanoparticles (LNPs) with entrapped essential oil (EO) from
cinnamon bark (Cinnamomum zeylanicum Blume), common thyme (Thymus vulgaris L.), and wild thyme (Thymus serpyllum L.) using a fast antisolvent method. Analysis of EO-loaded LNPs
by pyrolysis-gas chromatography–mass spectrometry and Fourier
transform infrared spectroscopy confirmed molecular interaction between
EOs and LNPs. Quantification of EO incorporation into the LNPs and
their in vitro release profiles were assessed by reversed phase high-performance
liquid chromatography. Utilized EOs were, to different extents, successfully
entrapped inside LNPs, which were attributed to extensive π-stacking
between aromatic compounds in EOs like cinnamaldehyde, thymol, and
carvacrol on one side and aromatic lignin units on the other side.
In vitro release of common thyme and wild thyme EOs from EO-loaded
LNPs was strongly delayed compared to the use of pure oil, giving
a promising outlook for the development of new bio-based biocide delivery
systems for wood preservation.