Deciphering the virulence factors of the opportunistic pathogen Mycobacterium colombiense

González-Pérez, Mónica; Murcia, Marta; Parra-López, Carlos; Blom, Jochen; Tauch, Andreas

doi:10.1016/j.nmni.2016.09.007

Cited by 5 publications

(7 citation statements)

References 49 publications

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“…Bioinformatic interrogation of the M. colombiense genome has uncovered a plethora of genes encoding putative virulence determinants . Two genetic loci merit consideration.…”

Section: Discussionmentioning

confidence: 99%

“…Two genetic loci merit consideration. First, a gene cluster that has been postulated to encode the synthesis of glycopeptidolipids . The production of these lipid moieties has been associated with a capacity of this organism to form biofilms.…”

Section: Discussionmentioning

confidence: 99%

“…5 Bioinformatic interrogation of the M. colombiense genome has uncovered a plethora of genes encoding putative virulence determinants. 15,16 Two genetic loci merit consideration. First, a gene cluster that has been postulated to encode the synthesis of glycopeptidolipids.…”

Section: Containment Of Mycobacterial Infections Relies On An Intactmentioning

confidence: 99%

“…First, a gene cluster that has been postulated to encode the synthesis of glycopeptidolipids. 15,16 The production of these lipid moieties has been associated with a capacity of this organism to form biofilms. A hallmark of these microbial communities that adhere to abiotic and biotic surfaces is a diminished susceptibility to anti-microbial agents.…”

Section: Containment Of Mycobacterial Infections Relies On An Intactmentioning

confidence: 99%

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A case report of fatal disseminated Mycobacterium colombiense infection in a renal transplant recipient

Gosal

Lee

2018

Transplant Infectious Dis

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“…Bioinformatic interrogation of the M. colombiense genome has uncovered a plethora of genes encoding putative virulence determinants . Two genetic loci merit consideration.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Containment Of Mycobacterial Infections Relies On An Intactmentioning

confidence: 99%

Section: Containment Of Mycobacterial Infections Relies On An Intactmentioning

confidence: 99%

See 2 more Smart Citations

A case report of fatal disseminated Mycobacterium colombiense infection in a renal transplant recipient

Gosal

Lee

2018

Transplant Infectious Dis

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“…Mycobacterium colombiense is an acid-fast, non-motile, rod-shaped mycobacterium that belongs to the Mycobacterium avium complex (MAC) [ 1 ]. MAC contains clinically important non-tuberculous mycobacteria (NTM) and is the second largest medical complex in the Mycobacterium genus after the Mycobacterium tuberculosis complex [ 2 ]. MAC comprises species that include M .…”

Section: Introductionmentioning

confidence: 99%

In silico analysis of promoter regions to identify regulatory elements in TetR family transcriptional regulatory genes of Mycobacterium colombiense CECT 3035

Hamde

Dinka

Naimuddin

2022

Journal of Genetic Engineering and Biotechnology

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Background Mycobacterium colombiense is an acid-fast, non-motile, rod-shaped mycobacterium confirmed to cause respiratory disease and disseminated infection in immune-compromised patients, and lymphadenopathy in immune-competent children. It has virulence mechanisms that allow them to adapt, survive, replicate, and produce diseases in the host. To tackle the diseases caused by M. colombiense, understanding of the regulation mechanisms of its genes is important. This paper, therefore, analyzes transcription start sites, promoter regions, motifs, transcription factors, and CpG islands in TetR family transcriptional regulatory (TFTR) genes of M. colombiense CECT 3035 using neural network promoter prediction, MEME, TOMTOM algorithms, and evolutionary analysis with the help of MEGA-X. Results The analysis of 22 protein coding TFTR genes of M. colombiense CECT 3035 showed that 86.36% and 13.64% of the gene sequences had one and two TSSs, respectively. Using MEME, we identified five motifs (MTF1, MTF2, MTF3, MTF4, and MTF5) and MTF1 was revealed as the common promoter motif for 100% TFTR genes of M. colombiense CECT 3035 which may serve as binding site for transcription factors that shared a minimum homology of 95.45%. MTF1 was compared to the registered prokaryotic motifs and found to match with 15 of them. MTF1 serves as the binding site mainly for AraC, LexA, and Bacterial histone-like protein families. Other protein families such as MATP, RR, σ-70 factor, TetR, LytTR, LuxR, and NAP also appear to be the binding candidates for MTF1. These families are known to have functions in virulence mechanisms, metabolism, quorum sensing, cell division, and antibiotic resistance. Furthermore, it was found that TFTR genes of M. colombiense CECT 3035 have many CpG islands with several fragments in their CpG islands. Molecular evolutionary genetic analysis showed close relationship among the genes. Conclusion We believe these findings will provide a better understanding of the regulation of TFTR genes in M. colombiense CECT 3035 involved in vital processes such as cell division, pathogenesis, and drug resistance and are likely to provide insights for drug development important to tackle the diseases caused by this mycobacterium. We believe this is the first report of in silico analyses of the transcriptional regulation of M. colombiense TFTR genes.

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Acquired and genetic host susceptibility factors and microbial pathogenic factors that predispose to nontuberculous mycobacterial infections

Honda¹,

Alper

Bai

et al. 2018

Current Opinion in Immunology

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Deciphering the virulence factors of the opportunistic pathogen Mycobacterium colombiense

Cited by 5 publications

References 49 publications

A case report of fatal disseminated Mycobacterium colombiense infection in a renal transplant recipient

A case report of fatal disseminated Mycobacterium colombiense infection in a renal transplant recipient

In silico analysis of promoter regions to identify regulatory elements in TetR family transcriptional regulatory genes of Mycobacterium colombiense CECT 3035

Acquired and genetic host susceptibility factors and microbial pathogenic factors that predispose to nontuberculous mycobacterial infections

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