Deciphering the roadmap of in vivo reprogramming toward pluripotency

Chondronasiou, Dafni; Villarreal, Jaime Martínez de; Melendez, Elena; Lynch, Cian J.; Pozo, Natalia del; Kovatcheva, Marta; Aguilera, Mònica; Prats, Neus; Real, Francisco X.; Serrano, Manuel

doi:10.1016/j.stemcr.2022.09.009

Cited by 15 publications

(10 citation statements)

References 57 publications

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“…Lineage trajectory analysis of this dataset suggested that the gastric-like population and tumour can arise through distinct cell states, but it is still not clear which metaplastic cell types become malignant. Interestingly, several Kras -induced gene signatures and populations were also identified in a scRNA-seq study by Chondronasiou et al where the authors used GEMMs overexpressing Yamanaka factors Oct4 , Sox2 , Klf4 and Myc , demonstrating that Kras G12D is sufficient to drive a common dedifferentiation programme 34…”

Section: Introductionmentioning

confidence: 83%

It is better to light a candle than to curse the darkness: single-cell transcriptomics sheds new light on pancreas biology and disease

Cephas

Hwang

Maitra

et al. 2023

Gut

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Recent advances in single-cell RNA sequencing and bioinformatics have drastically increased our ability to interrogate the cellular composition of traditionally difficult to study organs, such as the pancreas. With the advent of these technologies and approaches, the field has grown, in just a few years, from profiling pancreas disease states to identifying molecular mechanisms of therapy resistance in pancreatic ductal adenocarcinoma, a particularly deadly cancer. Single-cell transcriptomics and related spatial approaches have identified previously undescribed epithelial and stromal cell types and states, how these populations change with disease progression, and potential mechanisms of action which will serve as the basis for designing new therapeutic strategies. Here, we review the recent literature on how single-cell transcriptomic approaches have changed our understanding of pancreas biology and disease progression.

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Section: Introductionmentioning

confidence: 83%

It is better to light a candle than to curse the darkness: single-cell transcriptomics sheds new light on pancreas biology and disease

Cephas

Hwang

Maitra

et al. 2023

Gut

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“…Although we didn’t have H3K9me3 for this experiment the combination of DAPI and H3K9ac (complimentary to H3K9me3) suggests that we might be detecting heterochromatin changes. It will be informative to directly compare the performance of DNAm clocks and ImAge to quantify the apparent heterogeneity of in vivo reprogramming 135 .…”

Section: Discussionmentioning

confidence: 99%

ImAge: an imaging approach to quantitate aging and rejuvenation

Rodriguez¹,

Fiengo²,

Alvarez-Kuglen³

et al. 2022

Preprint

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Predictive biomarkers of functional or biological age are key for evaluating interventions aimed at increas-ing healthspan and / or lifespan in humans. Currently, cardiovascular performance, blood analytes, frailty indices (e.g. gait speed), and DNA methylation clocks are used to provide such estimates; each technique has its own challenges and limitations. We have developed a novel approach, microscopic imaging of bio-logical age (miBioAge), which computes multiparametric signatures based on the patterns of epigenetic landscape in single nuclei. We demonstrated that such miBioAge readouts can robustly distinguish young and old cells from multiple tissues, reveal aging progression in peripheral blood (e.g. CD3+ T cells), and reflect changes of epigenetic signatures consistent with expected slowdown or acceleration of biological age in chronologically identical mice treated with caloric restriction or chemotherapy. Because miBioAge readouts are computed from individual samples without applying linear regression, we posit that this novel biomarker may provide personalized assessment of functional aging.

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“…Some of the presented work has been published. [7][8][9] The paternal genome in a zygote undergoes active DNA demethylation before the first mitosis. 10 Dr. Guo-Liang Xu (Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences; Medical College of Fudan University) introduced his lab's early work on the demethylation of paternal DNA mediated by maternal Tet3 in zygotes.…”

Section: Dna Methylation In Rejuvenation Development and Cellular Sen...mentioning

confidence: 99%

“…Finally, Dr. Serrano presented factors that can either enhance or inhibit reprogramming efficiency. Some of the presented work has been published 7–9 …”

Section: Introductionmentioning

confidence: 99%

Promoting longevity with less cancer: The 2022 International Conference on Aging and Cancer

Cui,

Wang,

Jiang

et al. 2023

Aging and Cancer

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Aging and cancer are increasingly becoming big challenges for public health worldwide due to increased human life expectancy. Meanwhile, aging is one of the major risk factors for cancer. In December 2019, the first International Conference on Aging and Cancer was held in Haikou, Hainan province (island), China, preluding the establishment of the International Center for Aging and Cancer (ICAC) at Hainan, an institute dedicated to the research at the intersection of aging and cancer. Since then, the ICAC has hosted the annual conference each December in Hainan. The 2022 ICAC conference, with the theme of “promoting longevity with less cancer,” invited 17 internationally renowned scientists to share their new research and insights. Topics included DNA methylation in rejuvenation, development, and cellular senescence; lifespan regulation and longevity manipulation; metabolism and aging; cellular senescence and diseases; and novel therapeutics for cancer and antiaging/anticancer drug discovery. The forum highlighted the interconnectedness of aging and senescence with cancer evolution and risk. Although there is hope for preventing diseases like cancer by modulating systems that also control lifespan, attention has to be paid to the conflicting needs and competing demands in human biology.

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Deciphering the roadmap of in vivo reprogramming toward pluripotency

Cited by 15 publications

References 57 publications

It is better to light a candle than to curse the darkness: single-cell transcriptomics sheds new light on pancreas biology and disease

It is better to light a candle than to curse the darkness: single-cell transcriptomics sheds new light on pancreas biology and disease

ImAge: an imaging approach to quantitate aging and rejuvenation

Promoting longevity with less cancer: The 2022 International Conference on Aging and Cancer

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