Decidual Alkaline Phosphatase Activity in the Pregnant and Pseudopregnant Rat

Manning, John P.; Steinetz, Bernard G.; Giannina, T.

doi:10.1111/j.1749-6632.1969.tb46416.x

Cited by 25 publications

(10 citation statements)

References 56 publications

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“…Although there was about a twofold increase in the activity of this enzyme at the implantation sites between days 7 and 8, the main increase took place in the decidual nodules confirming previous findings (18,19,27) of a 10-fold increase in alkaline phosphatase activity on day 9 of pregnancy in the rat uterus, an increase localized exclusively to the implantation sites presumably containing the decidua. The biochemical findings also support the histochemical demonstra tions of increased alkaline phosphatase activity associated with decidualization (3,15,23).…”

Section: Discussionsupporting

confidence: 77%

Enzymic Activity in Rat Uterus during Early Pregnancy

Malinowska¹,

Fotherby²

1975

Horm Res

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Total protein, RNA and DNA content and the activity of acid and alkaline phosphatases, 5´-nucleotidase and isocitrate dehydrogenase were studied in rat uterus during the first 8 days of pregnancy. Isocitrate dehydrogenase activity showed marked fluctuations from day to day. Nucleotidase and acid phosphatase activities showed a significant increase on day 8. The most marked change in activity was that of alkaline phosphatase which showed a 10-fold increase between days 6 and 8, due largely to an increase in the activity of this enzyme in the decidual nodule. The rise in alkaline phosphatase activity did not occur in rats ovariectomized on days 1, 2 or 4 of pregnancy and was markedly decreased in those ovariectomized on day 6. [³H]-uridine incorporation into RNA showed a significant increase between days 2 and 6 whereas [³H]-thymidine incorporation into DNA showed a significant increase on day 6.

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Section: Discussionsupporting

confidence: 77%

Enzymic Activity in Rat Uterus during Early Pregnancy

Malinowska¹,

Fotherby²

1975

Horm Res

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“…It is unclear why these inhibitors did not prevent the ALP rise because the present studies show that there was a clear elevation of ALP mRNA correlating with the induction of ALP activity. 5-Flurodeoxyuridine and inhibitors of protein synthesis that inhibit DNA synthe¬ sis as a consequence of their actions also failed to reduce the decidual response in the studies of Manning et al (1969), despite the fact that decidualization involves substantial cell proliferation (Hall, 1969;Das & Martin, 1978). No measurements of the degree of inhibition of protein or RNA synthesis were performed by Manning et ai (1969).…”

Section: Discussionmentioning

confidence: 99%

“…The induction of enzyme activity was pre¬ ceded by an elevation in a 2-6 kb ALP mRNA which reached a peak 8-fold over the control level at Day 6. Manning et al (1969) were unable to inhibit the elevation of ALP-specific activity induced by traumatizing progesterone-primed rat uteri with inhibitors of transcription (actinomycin-D), replication (5-flurodeoxyuridine) or translation (cycloheximide, chloramphenicol). This led these workers to postulate that the ALP induction was due to post-translational activation of a pre¬ existing pro-enzyme.…”

Section: Discussionmentioning

confidence: 99%

Characterization and expression of uterine and placental alkaline phosphatases in the mouse

Pollard

Jahan²,

Butterworth³

1990

Reproduction

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Summary. Alkaline phosphatase (ALP) is rapidly induced in the uterine subepithelial stroma after a natural or artificial decidual stimulus. During gestation ALP-specific activity peaked at Day 7 to 8 (Day 1 is day of detection of the copulation plug) followed by a rapid decline to control levels by Day 9. This elevation in enzyme activity was preceded by an 8-fold induction of a 2\m=.\6kilobase (kb)

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“…An increase in alkaline phosphatase activity in the endometrial stroma is associated with decidualization (Lobel et al, 1965) and early pregnancy (Christie, 1966;Manning et al, 1969). This increase is maximal 24-72 h after the induction of decidualization and provides a good index of endometrial differentiation.…”

Section: Introductionmentioning

confidence: 98%

Evidence for a role for a uterine renin-angiotensin system in decidualization in rats

Squires¹,

Kennedy²

1992

Reproduction

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Experiments were performed in vivo and in vitro to determine the effects of enalaprilat, a specific inhibitor of angiotensin-converting enzyme, on various aspects of the decidual cell reaction in rats. Ovariectomized, adult female rats were sensitized for the decidual cell reaction with steroid treatments. For in vivo experiments, intrauterine infusions of enalaprilat alone, and in combination with angiotensin II and prostaglandin E2 (PGE2), were initiated on the day of uterine sensitivity. Enalaprilat inhibited the increases in uterine PG concentrations, endometrial vascular permeability, alkaline phosphatase activity and uterine weight that occurred sequentially following infusion of vehicle. Concurrent infusion of angiotensin II did not reverse any of these inhibitory effects; PGE2 infusion partially, but not completely, reversed the inhibition of increase in uterine weight, although it did not alter the inhibition of endometrial vascular permeability. For in vitro experiments, endometrial stromal cells were obtained from uteri on the day of sensitivity and cultured for up to 3 days in the presence of enalaprilat and angiotensin II. Enalaprilat inhibited in a dose-dependent manner the increases in stromal cell alkaline phosphatase activity and media PGE concentration that occurred in the control cultures; these effects were fully reversed by concurrent treatment with angiotensin II. The inhibition of stromal alkaline phosphatase activity was also reversed by PGE2; conversely, the ability of angiotensin II to reverse the effect of enalaprilat was lost in the presence of indomethacin. These studies provide evidence of a requirement for angiotensin II during the decidual cell reaction in rats and suggest that it acts, at least in part, through a PG-dependent mechanism.

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Decidual Alkaline Phosphatase Activity in the Pregnant and Pseudopregnant Rat

Cited by 25 publications

References 56 publications

Enzymic Activity in Rat Uterus during Early Pregnancy

Enzymic Activity in Rat Uterus during Early Pregnancy

Characterization and expression of uterine and placental alkaline phosphatases in the mouse

Evidence for a role for a uterine renin-angiotensin system in decidualization in rats

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