Decellularized silk fibroin scaffold primed with adipose mesenchymal stromal cells improves wound healing in diabetic mice

Navone, Stefania Elena; Pascucci, Luisa; Dossena, Marta; Ferri, Luca; Invernici, Gloria; Acerbi, Francesco; Cristini, Silvia; Bedini, Gloria; Tosetti, Valentina; Ceserani, Valentina; Bonomi, Andrea; Pessina, Augusto; Freddi, Giuliano; Alessandrino, Antonio; Ceccarelli, Piero; Campanella, Rolando; Marfia, Giovanni; Alessandri, Giulio; Parati, Eugenio

doi:10.1186/scrt396

Cited by 115 publications

(86 citation statements)

References 47 publications

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“…Silk fibroin scaffolds cellularized with MSCs or decellularized were grafted into wounds of diabetic mice. Both scaffolds improved wound healing and tissue regenera-tion and upregulated genes associated with angiogenesis and matrix remodeling [66]. Additionally, both scaffolds were able to promote endothelial cell migration by slightly different mechanisms.…”

Section: Cell Derived Ecmmentioning

confidence: 94%

Decellularized extracellular matrices for tissue engineering applications

Elmashhady¹,

Kraemer²,

Patel³

et al. 2017

Electrospinning

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Decellularization removes cellular antigens while preserving the ultrastructure and composition of extracellular matrix (ECM). Decellularized ECM (DECM) scaffolds have been widely used in various tissue engineering applications with varying levels of success. The mechanical, architectural and bioactive properties of a DECM scaffold depend largely on the method of decellularization and dictate its clinical efficacy. This article highlights the advantages and challenges associated with the clinical use of DECM scaffolds. Poor mechanical strength is a significant disadvantage of some DECM scaffolds in the repair of load-bearing tissues as well as critical-size defects, where long-term mechanical support is required for the regenerating tissue. Combining DECM scaffolds with synthetic biocompatible polymers could provide a useful strategy to circumvent the issues of poor mechanical stability. This article reviews studies that have combined DECM scaffolds from various tissues with synthetic polymers to create hybrid scaffolds using electrospinning. These hybrid scaffolds provide a mechanical backbone while retaining the bioactive properties of DECM, thus offering a significant advantage for tissue engineering and regenerative medicine applications.

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Section: Cell Derived Ecmmentioning

confidence: 94%

Decellularized extracellular matrices for tissue engineering applications

Elmashhady¹,

Kraemer²,

Patel³

et al. 2017

Electrospinning

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“…Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) have been shown to promote would healing by inducing angiogenesis and inhibiting prolonged inflammation [24]. However, these molecules are degraded quite fast by enzymes existing in the protease-rich wound environment [30].…”

Section: Biomaterials With Controlled-release Of Signaling Moleculesmentioning

confidence: 99%

“…Therefore, cellularized scaffolds based on above biomaterials were developed for diabetic wound healing [22,23]. Navone et al demonstrated electrospun nanofibrous Silk fibroin patches cellularized with human adipose-derived mesenchymal stem cells (ADMSCs) as an effective treatment for wound healing, which improved skin regeneration in diabetic mice [24]. ADMSCs were reported to confer benefits as tissue restorative agents in vivo.…”

Section: Hydrogel-based Biomaterialsmentioning

confidence: 99%

“…Fibrin [19] Collagen-GAG [20] Gelatin microcryogels [23] GAG [28] BLCC [29] Freeze-drying sponges pGIcNAc [18] Collagen sponge [27] Electrospun patches Collagen/PLGA [21] Silk fibroin [24] Biomaterials with controlledrelease of signaling molecules Nanoparticles loaded with GF/chemokines VEGF, bFGF nanoparticles in PEtU-PDMS/ fibrin [33] Curcumin loaded chitosan nanoparticles into collagen-alginate [36] With vectors encoding functional molecules AdeNOS in fibrin scaffold [37] Hyaluronic acid-MMP hydrogels with VEGF plasmids [34] GF/Chemokines preloadedd within Hydrogels Glucophage-loaded collagen/PLGA [21] Gelatin-PGA-based scaffold payload MCP-1 [35] healing benefit was associated with a more efficient vascular network. Breitbart et al seeded mouse dermal fibroblasts onto polyglycolic acid scaffold matrices, on which the cells were retrovirally transduced with the human platelet-derived growth factor B (PDGF-B) gene [28].…”

Section: High-water Content Hydrogelsmentioning

confidence: 99%

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Biomaterials for Promoting Wound Healing in Diabetes

Liu¹,

Zheng²,

Dai³

et al. 2017

J Tissue Sci Eng

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“…ASCs embedded in silk fibroin scaffolds and fibrin gels have demonstrated a similar ability to accelerate wound healing in vivo. 107,108 Autologous MSCs applied with a fibrin spray system also resulted in some improvement in patients with chronic ulcers. 93 Scaffolds offer a viable means for enhancing stem cell engraftment and survivability.…”

mentioning

confidence: 99%

Stem cells and chronic wound healing: state of the art

Leavitt¹,

Hu²,

Barnes³

et al. 2016

CWCMR

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Currently available treatments for chronic wounds are inadequate. A clearly effective therapy does not exist, and treatment is often supportive. This is largely because the cellular and molecular processes underlying failure of wound repair are still poorly understood. With an increase in comorbidities, such as diabetes and vascular disease, as well as an aging population, the incidence of these intractable wounds is expected to rise. As such, chronic wounds, which are already costly, are rapidly growing as a tremendous burden to the health-care system. Stem cells have garnered much interest as a therapy for chronic wounds due to their inherent ability to differentiate into multiple lineages and promote regeneration. Herein, we discuss the types of stem cells used for chronic wound therapy, as well as the proposed means by which they do so. In particular, we highlight mesenchymal stem cells (including adipose-derived stem cells), endothelial progenitor cells, and induced pluripotent stem cells. We include the results of recent in vitro and in vivo studies in both animal models and human clinical trials. Finally, we discuss the current studies to improve stem cell therapies and the limitations of stem cell-based therapeutics. Stem cells promise improved therapies for healing chronic wounds, but further studies that are well-designed with standardized protocols are necessary for fruition.

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Decellularized silk fibroin scaffold primed with adipose mesenchymal stromal cells improves wound healing in diabetic mice

Cited by 115 publications

References 47 publications

Decellularized extracellular matrices for tissue engineering applications

Decellularized extracellular matrices for tissue engineering applications

Biomaterials for Promoting Wound Healing in Diabetes

Stem cells and chronic wound healing: state of the art

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