Decellularized human umbilical artery: Biocompatibility and in vivo functionality in sheep carotid bypass model

Fang, Shu; Riber, Sara Schødt; Hussein, Kamal Hany; Ahlmann, Alexander Høgsted; Harvald, Eva Bang; Khan, Fazal; Beck, Hans Christian; Weile, Louise Katrine Kjær; Sørensen, Jens Ahm; Sheikh, Søren Paludan; Riber, Lars Peter; Andersen, Ditte Caroline

doi:10.1016/j.msec.2020.110955

Cited by 7 publications

(13 citation statements)

References 61 publications

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“…However, as previously shown by us in a human-sheep setup [5], we also herein observed a local response with CD45 + cells invading the dUA. This thus seems independent of the method of insertion [5], and points to a general response. Herein, we found that these CD45 + cells mainly comprise macrophages predominantly of the M2 polarization phenotype.…”

Section: Discussionsupporting

confidence: 89%

“…In this regard it is also worth noting that aldehyde fixed human umbilical vein, which include all the cell constituents in addition to the ECM scaffold, already has been tested rigorously in large diameter constructions in humans [31][32][33]. Previous [5] and present data thus suggest that our dUA are efficiently decellularised with preservation of ECM, but without M1 inducing determinants that may otherwise represent ineffective decellularisation [28].…”

Section: Discussionsupporting

confidence: 55%

“…However, small diameter (<6 mm) TEVGs still remain a major challenge in the field [ 2 ], where synthetic, natural and hybrid grafts cause unwanted reactions in the blood-material and tissue-material interfaces leading to thrombosis [ 3 ]. As an alternative, decellularised vessels are being explored, since they offer the correct 3D structure and natural extracellular matrix (ECM) composition with several decellularization methods already existing [ 4 , 5 ]. In this regard, we recently showed that human umbilical artery (UA) was successfully decellularised, easily handled by surgeons, and withstood the in vivo blood pressure in a sheep model for carotid bypass [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…As an alternative, decellularised vessels are being explored, since they offer the correct 3D structure and natural extracellular matrix (ECM) composition with several decellularization methods already existing [ 4 , 5 ]. In this regard, we recently showed that human umbilical artery (UA) was successfully decellularised, easily handled by surgeons, and withstood the in vivo blood pressure in a sheep model for carotid bypass [ 5 ]. The decellularised human UA holds several advantages over synthetic, autologous or cadaveric allogeneic sources of vascular grafts, providing readily available fresh tissue without invasive collection procedures.…”

Section: Introductionmentioning

confidence: 99%

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Decellularised Human Umbilical Artery as a Vascular Graft Elicits Minimal Pro-Inflammatory Host Response Ex Vivo and In Vivo

Ahlmann

Fang

Mortensen

et al. 2021

IJMS

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Small diameter (<6 mm) vessel grafts still pose a challenge for scientists worldwide. Decellularised umbilical artery (dUA) remains promising as small diameter tissue engineered vascular graft (TEVG), yet their immunogenicity remains unknown. Herein, we evaluated the host immune responses, with a focus on the innate part, towards human dUA implantation in mice, and confirmed our findings in an ex vivo allogeneic human setup. Overall, we did not observe any differences in the number of circulating white blood cells nor the number of monocytes among three groups of mice (1) dUA patch; (2) Sham; and (3) Mock throughout the study (day −7 to 28). Likewise, we found no difference in systemic inflammatory and anti-inflammatory cytokine levels between groups. However, a massive local remodelling response with M2 macrophages were observed in the dUA at day 28, whereas M1 macrophages were less frequent. Moreover, human monocytes from allogeneic individuals were differentiated into macrophages and exposed to lyophilised dUA to maximize an eventual M1 response. Yet, dUA did not elicit any immediate M1 response as determined by the absence of CCR7 and CXCL10. Together this suggests that human dUA elicits a minimal pro-inflammatory response further supporting its use as a TEVG in an allogeneic setup.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Decellularised Human Umbilical Artery as a Vascular Graft Elicits Minimal Pro-Inflammatory Host Response Ex Vivo and In Vivo

Ahlmann

Fang

Mortensen

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

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“…Many autografts, allografts, and xenografts contain elastic fibers. Well-known examples are split-skin autografts for burn wounds (56), autologous blood vessels allografts for coronary graft reconstruction (57), and aortic heart valve xenografts (58). Biofabrication methodologies provide access to numerous variants from elastin-based cellscaffolds.…”

Section: Elastin-based Biomaterialsmentioning

confidence: 99%

Recent Advances in Elastin-Based Biomaterials

et al. 2020

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Elastin is one of the main components of the extracellular matrix; it provides resistance and elasticity to a variety of tissues and organs of the human body, besides participating in cellular signaling. On the other hand, elastin-derived peptides are synthetic biopolymers with a similar conformation and structure to elastin, but these possess the advantage of solubility in aqueous mediums. Due to their biological activities and physicochemical properties, elastin and related peptides may be applied as biomaterials to develop diverse biomedical devices, including scaffolds, hydrogels, and drug delivery systems for tissue engineering. Likewise, the combination of elastin with natural or synthetic polymers has demonstrated to improve the mechanical properties of biomedical products and drug delivery systems. Here we comprehensively describe the physicochemical properties and physiological functions of elastin. Moreover, we offer an overview of the use of elastin and its derivative polymers as biomaterials to develop scaffolds and hydrogels for tissue engineering. Finally, we discuss some perspectives on the employment of these biopolymers to fabricate new biomedical products.

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Challenges and Strategies for Endothelializing Decellularized Small‐Diameter Tissue‐Engineered Vessel Grafts

Ding,

Hou,

Gao

et al. 2024

Adv Healthcare Materials

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Vascular diseases are the leading cause of ischemic necrosis in tissues and organs, necessitating using vascular grafts to restore blood supply. Currently, small vessels for coronary artery bypass grafts are unavailable in clinical settings. Decellularized small‐diameter tissue‐engineered vessel grafts (SD‐TEVGs) hold significant potential. However, they face challenges, as simple implantation of decellularized SD‐TEVGs in animals leads to thrombosis and calcification due to incomplete endothelialization. Consequently, research and development focus has shifted toward enhancing the endothelialization process of decellularized SD‐TEVGs. This paper reviews preclinical studies involving decellularized SD‐TEVGs, highlighting different strategies and their advantages and disadvantages for achieving rapid endothelialization of these vascular grafts. We analyzed methods to improve the process while addressing potential shortcomings. This paper aims to contribute to the future commercial viability of decellularized SD‐TEVGs.This article is protected by copyright. All rights reserved

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Decellularized human umbilical artery: Biocompatibility and in vivo functionality in sheep carotid bypass model

Cited by 7 publications

References 61 publications

Decellularised Human Umbilical Artery as a Vascular Graft Elicits Minimal Pro-Inflammatory Host Response Ex Vivo and In Vivo

Decellularised Human Umbilical Artery as a Vascular Graft Elicits Minimal Pro-Inflammatory Host Response Ex Vivo and In Vivo

Recent Advances in Elastin-Based Biomaterials

Challenges and Strategies for Endothelializing Decellularized Small‐Diameter Tissue‐Engineered Vessel Grafts

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