Decellularized heart ECM hydrogel using supercritical carbon dioxide for improved angiogenesis

Seo, Yoojin; Jung, Youngmee; Kim, Jeongrae

doi:10.1016/j.actbio.2017.11.046

Cited by 121 publications

(102 citation statements)

References 60 publications

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“…These constructs have the advantage of maintaining tissue‐specific cell functions and phenotypes to induce host tissue remodelling. Previous studies 23–38 have had success in obtaining aECM scaffolds from virtually all mammalian organs. They preserve the native vasculature for adequate perfusion of constructs able to withstand physiological blood pressures, something that has been a challenge to RM methods relying on progenitor cell embryogenesis 39,40 .…”

Section: State‐of‐the‐art Regenerative Medicine Technologiesmentioning

confidence: 99%

Regenerative medicine, organ bioengineering and transplantation

Edgar

Porter

et al. 2020

British Journal of Surgery

112

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Background Organ transplantation is predicted to increase as life expectancy and the incidence of chronic diseases rises. Regenerative medicine‐inspired technologies challenge the efficacy of the current allograft transplantation model. Methods A literature review was conducted using the PubMed interface of MEDLINE from the National Library of Medicine. Results were examined for relevance to innovations of organ bioengineering to inform analysis of advances in regenerative medicine affecting organ transplantation. Data reports from the Scientific Registry of Transplant Recipient and Organ Procurement Transplantation Network from 2008 to 2019 of kidney, pancreas, liver, heart, lung and intestine transplants performed, and patients currently on waiting lists for respective organs, were reviewed to demonstrate the shortage and need for transplantable organs. Results Regenerative medicine technologies aim to repair and regenerate poorly functioning organs. One goal is to achieve an immunosuppression‐free state to improve quality of life, reduce complications and toxicities, and eliminate the cost of lifelong antirejection therapy. Innovative strategies include decellularization to fabricate acellular scaffolds that will be used as a template for organ manufacturing, three‐dimensional printing and interspecies blastocyst complementation. Induced pluripotent stem cells are an innovation in stem cell technology which mitigate both the ethical concerns associated with embryonic stem cells and the limitation of other progenitor cells, which lack pluripotency. Regenerative medicine technologies hold promise in a wide array of fields and applications, such as promoting regeneration of native cell lines, growth of new tissue or organs, modelling of disease states, and augmenting the viability of existing ex vivo transplanted organs. Conclusion The future of organ bioengineering relies on furthering understanding of organogenesis, in vivo regeneration, regenerative immunology and long‐term monitoring of implanted bioengineered organs.

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Section: State‐of‐the‐art Regenerative Medicine Technologiesmentioning

confidence: 99%

Regenerative medicine, organ bioengineering and transplantation

Edgar

Porter

et al. 2020

British Journal of Surgery

112

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“…Physical methods rely on using freeze‐thaw cycles, agitation, or application of pressure to damage cells and allow for cell removal, and are commonly used in conjunction with physical or enzymatic methods to improve decellularization . Regardless of method, complete decellularization is readily available for applications of dECM materials in cardiac tissue engineering …”

Section: Solid Decmmentioning

confidence: 99%

“…Compared to venting the heart apex during decellularization, the inversion method showed higher coronary perfusion efficiency, better cellular and DNA removal, higher collagen and elastin content in the aortic valve, and better shape retention. Seo et al used a decellularization method based on supercritical CO 2 and ethanol to preserve collagen, laminin, GAGs, fibronectin, and angiogenic GFs more effectively than detergent methods . Merna et al used multiphoton microscopy and image correlation spectroscopy to noninvasively characterize the mechanical and structural properties of whole hearts during decellularization .…”

Section: Solid Decmmentioning

confidence: 99%

“…In addition, various fabrication techniques can be employed for generating cardiac patches with cellular or additional biomaterial integration. Soluble dECM has been generated from cECM, pECM, sECM, SIS, placenta, and omentum from a variety of animal sources for cardiovascular applications . Decellularization of organ tissue is followed using similar protocols as implemented for solid dECM, where detergent methods are most often used.…”

Section: Soluble Decmmentioning

confidence: 99%

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Decellularized Extracellular Matrix Materials for Cardiac Repair and Regeneration

Bejleri

Davis

2019

Adv Healthcare Materials

141

105

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Decellularized extracellular matrix (dECM) is a promising biomaterial for repairing cardiovascular tissue, as dECM most effectively captures the complex array of proteins, glycosaminoglycans, proteoglycans, and many other matrix components that are found in native tissue, providing ideal cues for regeneration and repair of damaged myocardium. dECM can be used in a variety of forms, such as solid scaffolds that maintain native matrix structure, or as soluble materials that can form injectable hydrogels for tissue repair. dECM has found recent success in many regeneration and repair therapies, such as for musculoskeletal, neural, and liver tissues. This review focuses on dECM in the context of cardiovascular applications, with variations in tissue and species sourcing, and specifically discusses advances in solid and soluble dECM development, in vitro studies, in vivo implementation, and clinical translation.

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“…Decellularized matrix scaffolds have been widely utilized to repair ischemic tissues and promote tissue regeneration [20][21][22]. e prevailing advantage of decellularized matrix scaffolds is their similar properties with the native extracellular matrix, thus, providing a proper microenvironment for cell survival, retention, and engraftment of transplanted stem cells [23].…”

Section: Discussionmentioning

confidence: 99%

Decellularized Aortic Scaffold Alleviates H₂O₂-Induced Inflammation and Apoptosis in CD34+ Progenitor Cells While Driving Neovasculogenesis

Gao

Feng

Cui

et al. 2020

BioMed Research International

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Bone marrow-derived stem/progenitor cells have been utilized for cardiac or vascular repair after ischemic injury, but they are subject to apoptosis and immune rejection in the ischemic site. Multiple scaffolds were used as delivery tools to transplant stem/ progenitor cells; however, these scaffolds did not show intrinsically antiapoptotic or anti-inflammatory properties. Decellularized aortic scaffolds that facilitate cell delivery and tissue repair were prepared by removing cells of patient-derived aortic tissues. Scanning electron microscopy (SEM) showed cells attached well to the scaffold after culturing for 5 days. Live/dead staining showed most seeded cells survived at day 7 on a decellularized aortic scaffold. Ki67 staining demonstrated that decellularized aortic scaffold promoted proliferation of bone marrow-derived CD34+ progenitor cells. Apoptosis of CD34+ progenitor cells induced by H 2 O 2 at high concentration was significantly alleviated in the presence of decellularized aortic scaffolds, demonstrating a protective effect against oxidative stress-induced apoptosis. Furthermore, decellularized aortic scaffolds significantly reduced the expression of proinflammatory cytokines (IL-8, GM-CSF, MIP-1β, GRO-α, Entoxin, and GRO) concurrently with an increase in anti-inflammatory cytokines (IL-2 and TGF-β) released from CD34+ progenitor cells when exposed to H 2 O 2 at low concentration. Finally, neovascularization was observed by H&E and immunohistochemical staining 14 days after the decellularized aortic scaffolds were subcutaneously implanted in nude mice. is preclinical study demonstrates that the use of a decellularized aortic scaffold possessing antiapoptotic and anti-inflammatory properties may represent a promising strategy for cardiovascular repair after ischemic injury.

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Decellularized heart ECM hydrogel using supercritical carbon dioxide for improved angiogenesis

Cited by 121 publications

References 60 publications

Regenerative medicine, organ bioengineering and transplantation

Regenerative medicine, organ bioengineering and transplantation

Decellularized Extracellular Matrix Materials for Cardiac Repair and Regeneration

Decellularized Aortic Scaffold Alleviates H₂O₂-Induced Inflammation and Apoptosis in CD34+ Progenitor Cells While Driving Neovasculogenesis

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Decellularized heart ECM hydrogel using supercritical carbon dioxide for improved angiogenesis

Cited by 121 publications

References 60 publications

Regenerative medicine, organ bioengineering and transplantation

Regenerative medicine, organ bioengineering and transplantation

Decellularized Extracellular Matrix Materials for Cardiac Repair and Regeneration

Decellularized Aortic Scaffold Alleviates H2O2-Induced Inflammation and Apoptosis in CD34+ Progenitor Cells While Driving Neovasculogenesis

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Product

Resources

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Decellularized Aortic Scaffold Alleviates H₂O₂-Induced Inflammation and Apoptosis in CD34+ Progenitor Cells While Driving Neovasculogenesis