Decellularized ECM hydrogels: prior use considerations, applications, and opportunities in tissue engineering and biofabrication

Kort-Mascort, Jacqueline; Flores-Torres, Salvador; Peza-Chavez, Omar; Jang, Joyce H; Pardo, Lucas Antonio; Tran, Simon D.; Kinsella, Joseph M.

doi:10.1039/d2bm01273a

Cited by 20 publications

(14 citation statements)

References 329 publications

(600 reference statements)

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“…To create dECM hydrogels, the decellularized tissue is solubilized via enzymatic digestion. , The resulting dECM hydrogel loses some of the mechanical integrity of the issue of origin, thus it can be combined with other biomaterials to improve its stability. dECM hydrogels are thermosensitive, they show an increase in moduli at physiological temperature as a result of an entropy-driven process known as collagen kinetics .…”

Section: Bioprinting the Tumor Microenvironmentmentioning

confidence: 99%

Constructing 3D In Vitro Models of Heterocellular Solid Tumors and Stromal Tissues Using Extrusion-Based Bioprinting

Flores-Torres

Jiang

Kort-Mascort

et al. 2023

ACS Biomater. Sci. Eng.

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Malignant tumor tissues exhibit inter- and intratumoral heterogeneities, aberrant development, dynamic stromal composition, diverse tissue phenotypes, and cell populations growing within localized mechanical stresses in hypoxic conditions. Experimental tumor models employing engineered systems that isolate and study these complex variables using in vitro techniques are under development as complementary methods to preclinical in vivo models. Here, advances in extrusion bioprinting as an enabling technology to recreate the three-dimensional tumor milieu and its complex heterogeneous characteristics are reviewed. Extrusion bioprinting allows for the deposition of multiple materials, or selected cell types and concentrations, into models based upon physiological features of the tumor. This affords the creation of complex samples with representative extracellular or stromal compositions that replicate the biology of patient tissue. Biomaterial engineering of printable materials that replicate specific features of the tumor microenvironment offer experimental reproducibility, throughput, and physiological relevance compared to animal models. In this review, we describe the potential of extrusion-based bioprinting to recreate the tumor microenvironment within in vitro models.

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Section: Bioprinting the Tumor Microenvironmentmentioning

confidence: 99%

Constructing 3D In Vitro Models of Heterocellular Solid Tumors and Stromal Tissues Using Extrusion-Based Bioprinting

Flores-Torres

Jiang

Kort-Mascort

et al. 2023

ACS Biomater. Sci. Eng.

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“…Combining soluble dECM with other polymers to form heterogeneous hydrogels with adjustable degradation rates is an important area of research for future dECM usage. 150,182,183 The mechanism of action of dECM in the treatment of IDD is still unclear and may be related to stem cell recruitment, biomechanics and biochemical signalling of dECM, 182 and a better understanding of the role of the ECM ecological microenvironment in IVD repair will help to advance research in IDD prevention and treatment. Injection of hydrogels into the IVD requires acupuncture for AF, which may aggravate IDD.…”

Section: Biomaterials Sciencementioning

confidence: 99%

Research progress in decellularized extracellular matrix hydrogels for intervertebral disc degeneration

Peng

Zhang

et al. 2023

Biomater. Sci.

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“…Both of them will be melted to form a gel or liquid. This procedure can be achieved by generating hydrogels from decellularized tissues via enzymatic and acidic digestions [164]. It has been shown that collagen type I can change into crystal liquid phases when it is concentrated in an acid solution, which may be a limiting factor [165].…”

Section: Hypothesis Of Proposed Model For Keratoprosthesesmentioning

confidence: 99%

A Proposed Model of Xeno-Keratoplasty Using 3D Printing and Decellularization

Wang¹,

Elbahrawi²,

Abdukadir³

et al. 2023

Preprint

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Corneal opacity is a leading cause of vision impairment and suffering worldwide. Transplantation can effectively restore vision and reduce chronic discomfort. However, there is a considerable shortage of viable corneal graft tissues. Tissue engineering may address this issue by advancing xeno- keratoplasty as a viable alternative to conventional keratoplasty. In particular, livestock decellularization strategies offer the potential to generate bioartificial ocular prosthetics in sufficient supply to match existing and projected needs. To this end, we have examined the best practices and characterizations that have supported the current state-of-the-art driving preclinical and clinical applications. Identifying the challenges that delimit activities to supplement the donor corneal pool derived from acellular scaffolds allowed us to hypothesize a model for keratoprosthesis applications derived from livestock combining 3D printing and decellularization.

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Decellularized ECM hydrogels: prior use considerations, applications, and opportunities in tissue engineering and biofabrication

Abstract: Tissue development, wound healing, pathogenesis, regeneration, and homeostasis rely upon coordinated and dynamic spatial and temporal remodeling of extracellular matrix (ECM) molecules. ECM reorganization and normal physiological tissue function, require...

Cited by 20 publications

References 329 publications

Constructing 3D In Vitro Models of Heterocellular Solid Tumors and Stromal Tissues Using Extrusion-Based Bioprinting

Constructing 3D In Vitro Models of Heterocellular Solid Tumors and Stromal Tissues Using Extrusion-Based Bioprinting

Research progress in decellularized extracellular matrix hydrogels for intervertebral disc degeneration

A Proposed Model of Xeno-Keratoplasty Using 3D Printing and Decellularization

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