Decellularization of the human donor aortic conduit by a new hybrid treatment in a multicomponent system with supercritical CO2 and Tween 80

Chaschin, Ivan S.; Бритиков, Д.В.; Хугаев, Г.А.; Salokhedinova, Regina R.; Зубко, А.В.; Abramchuk, S. S.; Petlenko, Antonina A.; Муратов, Р.М.; Bakuleva, Natalia P.

doi:10.1016/j.supflu.2021.105452

Cited by 6 publications

(4 citation statements)

References 48 publications

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“…As such, further investigations into the potential of PL to act as a decellularizing agent are necessary. The only nonionic detergents other than Triton X-100 to be investigated for use in decellularization are Tween 20 and Tween 80, both of which consistently produce inadequate decellularizations apart from a study by Chaschin et al 67 who found that inclusion of Tween 80 in decellularization of human aorta by supercritical carbon dioxide (scCO2) was beneficial. [64][65][66] Zwitterionic detergents share properties with both ionic and nonionic detergents.…”

Section: Chemical Treatment-based Decellularizationmentioning

confidence: 99%

Decellularization for the retention of tissue niches

Moffat

Jin

2022

J Tissue Eng

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Decellularization of natural tissues to produce extracellular matrix is a promising method for three-dimensional scaffolding and for understanding microenvironment of the tissue of interest. Due to the lack of a universal standard protocol for tissue decellularization, recent investigations seek to develop novel methods for whole or partial organ decellularization capable of supporting cell differentiation and implantation towards appropriate tissue regeneration. This review provides a comprehensive and updated perspective on the most recent advances in decellularization strategies for a variety of organs and tissues, highlighting techniques of chemical, physical, biological, enzymatic, or combinative-based methods to remove cellular contents from tissues. In addition, the review presents modernized approaches for improving standard decellularization protocols for numerous organ types.

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Section: Chemical Treatment-based Decellularizationmentioning

confidence: 99%

Decellularization for the retention of tissue niches

Moffat

Jin

2022

J Tissue Eng

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“…In contrast, a significant amount of cytoplasm was noticed to be scattered across the ECM, especially in Tw-20 ( Figures 4C, D ) and CHAPS tissue ( Figures 4E, F ), both in ID and PD. Since Tw-20 is a mild detergent known to solubilize lipids and proteins under specified conditions, it was recognized that it decellularizes tissue slowly and leaves cytoplasm behind, making it an unsuitable option for decellularization ( Chaschin et al, 2022 ). In ID, TX-100 exhibited shrunken cytoplasm, which could be seen adhering to the broken ECM network ( Figure 4G ), while SDS ( Figure 4I ) and SDC ( Figure 4K ) have the same shrunken cytoplasm accompanied with discrete strands of ECM.…”

Section: Resultsmentioning

confidence: 99%

Impact of various detergent-based immersion and perfusion decellularization strategies on the novel caprine pancreas derived extracellular matrix scaffold

Singh,

Satpathi,

Gopala Reddy

et al. 2023

Front. Bioeng. Biotechnol.

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Limited availability of the organs donors has facilitated the establishment of xenogeneic organ sources for transplantation. Numerous studies have decellularized several organs and assessed their implantability in order to provide such organs. Among all the decellularized organs studies for xenotransplantation, the pancreas has garnered very limited amount of research. The presently offered alternatives for pancreas transplantation are unable to liberate patients from donor dependence. The rat and mice pancreas are not of an accurate size for transplantation but can only be used for in-vitro studies mimicking in-vivo immune response in humans, while the porcine pancreas can cause zoonotic diseases as it carries porcine endogenous retrovirus (PERV- A/B/C). Therefore, we propose caprine pancreas as a substitute for these organs, which not only reduces donor dependence but also poses no risk of zoonosis. Upon decellularization the extracellular matrix (ECM) of different tissues responds differently to the detergents used for decellularization at physical and physiological level; this necessitates a comprehensive analysis of each tissue independently. This study investigates the impact of decellularization by ionic (SDS and SDC), non-ionic (Triton X-100 and Tween-20), and zwitterionic detergents (CHAPS). All these five detergents have been used to decellularize caprine pancreas via immersion (ID) and perfusion (PD) set-up. In this study, an extensive comparison of these two configurations (ID and PD) with regard to each detergent has been conducted. The final obtained scaffold with each set-up has been evaluated for the left-over cytosolic content, ECM components like sGAG, collagen, and fibronectin were estimated via Prussian blue and Immunohistochemical staining respectively, and finally for the tensile strength and antimicrobial activity. All the detergents performed consistently superior in PD than in ID. Conclusively, PD with SDS, SDC, and TX-100 successfully decellularizes caprine pancreatic tissue while retaining ECM architecture and mechanical properties. This research demonstrates the viability of caprine pancreatic tissue as a substitute scaffold for porcine organs and provides optimal decellularization protocol for this xenogeneic tissue. This research aims to establish a foundation for further investigations into potential regenerative strategies using this ECM in combination with other factors.

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“…There is plenty of research considering synthetic materials for vascular grafting [1,2,[27][28][29][30][31][32]. However, opinions on the importance of complete resemblance of native vessel biomechanics before the graft vitalization were divided.…”

Section: Discussionmentioning

confidence: 99%

“…Calcification is one of the most frequent complications of vascular grafting. This problem is not specific to artificial scaffolds but is also relevant to xenogeneic and allogeneic materials [26][27][28][29][30][31]. Scientists have been facing implant mineralization in different animal models, with devices made of different materials [31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Remote Outcomes with Poly-ε-Caprolactone Aortic Grafts in Rats

Dokuchaeva,

Mochalova,

Timchenko

et al. 2023

Polymers

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Poly-ε-caprolactone ((1,7)-polyoxepan-2-one; PCL) is a biodegradable polymer widely used in various fields of bioengineering, but its behavior in long-term studies appears to depend on many conditions, such as application specificity, chemical structure, in vivo test systems, and even environmental conditions in which the construction is exploited in. In this study, we offer an observation of the remote outcomes of PCL tubular grafts for abdominal aorta replacement in an in vivo experiment on a rat model. Adult Wistar rats were implanted with PCL vascular matrices and observed for 180 days. The results of ultrasound diagnostics and X-ray tomography (CBCT) show that the grafts maintained patency for the entire follow-up period without thrombosis, leakage, or interruptions, but different types of tissue reactions were found at this time point. By the day of examination, all the implants revealed a confluent endothelial monolayer covering layers of hyperplastic neointima formed on the luminal surface of the grafts. Foreign body reactions were found in several explants including those without signs of stenosis. Most of the scaffolds showed a pronounced infiltration with fibroblastic cells. All the samples revealed subintimal calcium phosphate deposits. A correlation between chondroid metaplasia in profound cells of neointima and the process of mineralization was supported by immunohistochemical (IHC) staining for S100 proteins and EDS mapping. Microscopy showed that the scaffolds with an intensive inflammatory response or formed fibrotic capsules retain their fibrillar structure even on day 180 after implantation, but matrices infiltrated with viable cells partially save the original fibrillary network. This research highlights the advantages of PCL vascular scaffolds, such as graft permeability, revitalization, and good surgical outcomes. The disadvantages are low biodegradation rates and exceptionally high risks of mineralization and intimal hyperplasia.

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Decellularization of the human donor aortic conduit by a new hybrid treatment in a multicomponent system with supercritical CO2 and Tween 80

Cited by 6 publications

References 48 publications

Decellularization for the retention of tissue niches

Decellularization for the retention of tissue niches

Impact of various detergent-based immersion and perfusion decellularization strategies on the novel caprine pancreas derived extracellular matrix scaffold

Remote Outcomes with Poly-ε-Caprolactone Aortic Grafts in Rats

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