Decellularization for the retention of tissue niches

Moffat, Deana; Ye, Kaiming; Jin, Sha

doi:10.1177/20417314221101151

Cited by 65 publications

(61 citation statements)

References 301 publications

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“…In nature, various cell types and the ECM form an organ-specific microenvironment [ 53 , 54 ]. Therefore, one paradigm in tissue engineering and the bioprinting of functional tissue is to resemble this natural microenvironment to maintain the physiological functionality of effector cells [ 4 , 53 , 54 , 55 , 56 ]. There are numerous studies on bioink formulations that specifically support effector cell viability and function [ 24 ].…”

Section: Strategies Before 3d Bioprinting: Materials Properties and B...mentioning

confidence: 99%

Vascularization in Bioartificial Parenchymal Tissue: Bioink and Bioprinting Strategies

Salg

Blaeser

Gerhardus

et al. 2022

IJMS

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Among advanced therapy medicinal products, tissue-engineered products have the potential to address the current critical shortage of donor organs and provide future alternative options in organ replacement therapy. The clinically available tissue-engineered products comprise bradytrophic tissue such as skin, cornea, and cartilage. A sufficient macro- and microvascular network to support the viability and function of effector cells has been identified as one of the main challenges in developing bioartificial parenchymal tissue. Three-dimensional bioprinting is an emerging technology that might overcome this challenge by precise spatial bioink deposition for the generation of a predefined architecture. Bioinks are printing substrates that may contain cells, matrix compounds, and signaling molecules within support materials such as hydrogels. Bioinks can provide cues to promote vascularization, including proangiogenic signaling molecules and cocultured cells. Both of these strategies are reported to enhance vascularization. We review pre-, intra-, and postprinting strategies such as bioink composition, bioprinting platforms, and material deposition strategies for building vascularized tissue. In addition, bioconvergence approaches such as computer simulation and artificial intelligence can support current experimental designs. Imaging-derived vascular trees can serve as blueprints. While acknowledging that a lack of structured evidence inhibits further meta-analysis, this review discusses an end-to-end process for the fabrication of vascularized, parenchymal tissue.

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Section: Strategies Before 3d Bioprinting: Materials Properties and B...mentioning

confidence: 99%

Vascularization in Bioartificial Parenchymal Tissue: Bioink and Bioprinting Strategies

Salg

Blaeser

Gerhardus

et al. 2022

IJMS

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“…In addition, fibronectins, glycosaminoglycans, proteoglycans, and extracellular matrix regulators and secreted factors are easily lost during SDS decellularization. 11 …”

Section: Introductionmentioning

confidence: 99%

“…As a result, artificial blood vessels are often employed, but those currently in use have considerable limitations. Therefore, decellularized tissues have been widely studied as a new biomaterial for various tissues and organs. − Decellularized tissues are widely accepted because their biocompatibility and regenerative properties are better than those of other non-natural engineered tissues. For decellularized tissues to play a major role in regenerative medicine, the basic properties of decellularized tissues must be investigated.…”

Section: Introductionmentioning

confidence: 99%

“…The simplest and most commonly used decellularization method combines lipid removal by SDS and DNA fragmentation by DNase. − SDS is a powerful detergent that can effectively remove lipids from tissue. Therefore, when DNase treatment is applied following SDS treatment, DNA can be fragmented efficiently.…”

Section: Introductionmentioning

confidence: 99%

“…However, similar to the experience of skin irritation from washing dishes, SDS is a substance that causes inflammation when it remains in decellularized tissues and therefore must be thoroughly removed from decellularized tissues. In addition, fibronectins, glycosaminoglycans, proteoglycans, and extracellular matrix regulators and secreted factors are easily lost during SDS decellularization …”

Section: Introductionmentioning

confidence: 99%

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Tensile Strength of Porcine Aorta Decellularized with Liquefied Dimethyl Ether and DNase

et al. 2022

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In a previous report, we proposed a method for decellularizing porcine aortas by removing lipids from the aortas using liquefied dimethyl ether (DME) instead of the conventional sodium dodecyl sulfate (SDS). This is followed by DNA fragmentation with DNase. In the current work, the physical properties of porcine aortas decellularized using the DME method are evaluated by tensile strength tests. Conventional SDS decellularized aortas are typically swollen, rupture very easily, and have poor elasticity. By contrast, DME-treated samples are found to be less elastic. However, the maximum stress required for rupture is greater than that for the original aorta. These results indicate that decellularization with DME and DNase increases the maximum stress that can be withstood. Reduction of elasticity may derive from the appearance of temporary C=N bonds due to Schiff-base reactions that occur during the lipid removal process by liquefied DME, and methods to avoid this are desirable.

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Innovative Substrate Design with Basement Membrane Components for Enhanced Endothelial Cell Function and Endothelization

Snyder,

Jana

2024

Adv Healthcare Materials

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Enhancing endothelial cell growth on small‐diameter vascular grafts produced from decellularized tissues or synthetic substrates is pivotal for preventing thrombosis. While optimized decellularization protocols can preserve the structure and many components of the extracellular matrix (ECM), the process can still lead to the loss of crucial basement membrane proteins, such as laminin, collagen IV, and perlecan, which are pivotal for endothelial cell adherence and functional growth. This loss can result in poor endothelialization and endothelial cell activation causing thrombosis and intimal hyperplasia. To address this, the basement membrane's ECM is emulated on fiber substrates, providing a more physiological environment for endothelial cells. Thus, fibroblasts are cultured on fiber substrates to produce an ECM membrane substrate (EMMS) with basement membrane proteins. The EMMS then underwent antigen removal (AR) treatment to eliminate antigens from the membrane while preserving essential proteins and producing an AR‐treated membrane substrate (AMS). Subsequently, human endothelial cells cultured on the AMS exhibited superior proliferation, nitric oxide production, and increased expression of endothelial markers of quiescence/homeostasis, along with autophagy and antithrombotic factors, compared to those on the decellularized aortic tissue. This strategy showed the potential of pre‐endowing fiber substrates with a basement membrane to enable better endothelization.

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Decellularization for the retention of tissue niches

Cited by 65 publications

References 301 publications

Vascularization in Bioartificial Parenchymal Tissue: Bioink and Bioprinting Strategies

Vascularization in Bioartificial Parenchymal Tissue: Bioink and Bioprinting Strategies

Tensile Strength of Porcine Aorta Decellularized with Liquefied Dimethyl Ether and DNase

Innovative Substrate Design with Basement Membrane Components for Enhanced Endothelial Cell Function and Endothelization

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