Decellularization of Chondrocyte-Encapsulated Collagen Microspheres: A Three-Dimensional Model to Study the Effects of Acellular Matrix on Stem Cell Fate

Cheng, Haobo; Tsui, Yuen-Kee; Cheung, Kenneth M.C.; Chan, Danny; Chan, Barbara Pui

doi:10.1089/ten.tec.2008.0635

Cited by 80 publications

(67 citation statements)

References 24 publications

(24 reference statements)

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“…Similar findings of GAG loss have been reported for different types of tissues decellularized using SDS. 44,45 Since GAG is the major component in the spongiosa of the pulmonary leaflets it was important to examine the effect of GAG loss on the hydrodynamic function of the acellular valves.…”

Section: Figmentioning

confidence: 99%

Development and Characterization of Acellular Porcine Pulmonary Valve Scaffolds for Tissue Engineering

Luo

Korossis

Wilshaw

et al. 2014

Tissue Engineering Part A

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Currently available replacement heart valves all have limitations. This study aimed to produce and characterize an acellular, biocompatible porcine pulmonary root conduit for reconstruction of the right ventricular outflow tract e.g., during Ross procedure. A process for the decellularization of porcine pulmonary roots was developed incorporating trypsin treatment of the adventitial surface of the scraped pulmonary artery and sequential treatment with hypotonic Tris buffer (HTB; 10 mM Tris pH 8.0, 0.1% (w/v) EDTA, and 10 KIU aprotinin), 0.1% (w/v) sodium dodecyl sulfate in HTB, two cycles of DNase and RNase, and sterilization with 0.1% (v/v) peracetic acid. Histology confirmed an absence of cells and retention of the gross histoarchitecture. Immunohistochemistry further confirmed cell removal and partial retention of the extracellular matrix, but a loss of collagen type IV. DNA levels were reduced by more than 96% throughout all regions of the acellular tissue and no functional genes were detected using polymerase chain reaction. Total collagen levels were retained but there was a significant loss of glycosaminoglycans following decellularization. The biomechanical, hydrodynamic, and leaflet kinematics properties were minimally affected by the process. Both immunohistochemical labeling and antibody absorption assay confirmed a lack of a-gal epitopes in the acellular porcine pulmonary roots and in vitro biocompatibility studies indicated that acellular leaflets and pulmonary arteries were not cytotoxic. Overall the acellular porcine pulmonary roots have excellent potential for development of a tissue substitute for right ventricular outflow tract reconstruction e.g., during the Ross procedure.

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Section: Figmentioning

confidence: 99%

Development and Characterization of Acellular Porcine Pulmonary Valve Scaffolds for Tissue Engineering

Luo

Korossis

Wilshaw

et al. 2014

Tissue Engineering Part A

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“…4,5 ECM is the unique product of resident cells relevant to its tissue localization, and influences stem cell fate by adjusting cell-matrix interactions. 6,7 Recently, more and more ECM-derived scaffolds have been examined and fabricated to offer a specific environment for cartilage repair or regeneration.…”

mentioning

confidence: 99%

Cartilage fragments from osteoarthritic knee promote chondrogenesis of mesenchymal stem cells without exogenous growth factor induction

Chen

Liao

Wang

et al. 2011

Journal Orthopaedic Research

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Extracellular matrix (ECM) is thought to participate significantly in guiding the differentiation process of mesenchymal stem cells (MSCs). In this study, we hypothesized that cartilage fragments from osteoarthritic knee could promote chondrogenesis of MSCs. Nonworn parts of cartilage tissues were obtained during total knee arthroplasty (TKA) surgery. Cartilage fragments and MSCs were wrapped into fibrin glue; and the constructs were implanted subcutaneously into nude mice. Histological analysis showed neocartilage-like structure with positive Alcian blue staining in the cartilage fragment-fibrin-MSC constructs. However, constructs with only MSCs in fibrin showed condensed appearance like MSCs in the pellet culture. Gene expression of type II collagen in the constructs with 60 mg cartilage fragments were significantly elevated after 4 weeks of implantation. Conversely, the constructs without cartilage fragments failed to express type II collagen, which indicated MSCs did not differentiate into a chondrogenic lineage. In conclusion, we demonstrated the effect of cartilage fragments from osteoarthritic knee in promoting chondrogenic differentiation of MSCs. This may be a favorable strategy for MSC chondrogenesis without exogenous growth factor induction. ß

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“…These valuable properties are frequently exploited for tissue engineering applications as well as for improvement of diabetic wound healing [5][6][7].…”

Section: Tissue-derived Scaffoldsmentioning

confidence: 99%

Biomaterials for Promoting Wound Healing in Diabetes

Liu¹,

Zheng²,

Dai³

et al. 2017

J Tissue Sci Eng

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Decellularization of Chondrocyte-Encapsulated Collagen Microspheres: A Three-Dimensional Model to Study the Effects of Acellular Matrix on Stem Cell Fate

Cited by 80 publications

References 24 publications

Development and Characterization of Acellular Porcine Pulmonary Valve Scaffolds for Tissue Engineering

Development and Characterization of Acellular Porcine Pulmonary Valve Scaffolds for Tissue Engineering

Cartilage fragments from osteoarthritic knee promote chondrogenesis of mesenchymal stem cells without exogenous growth factor induction

Biomaterials for Promoting Wound Healing in Diabetes

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