Development and Characterization of Acellular Porcine Pulmonary Valve Scaffolds for Tissue Engineering

Luo, Ji; Korossis, Sotirios; Wilshaw, Stacy‐Paul; Jennings, Louise; Fisher, John; Ingham, Eileen

doi:10.1089/ten.tea.2013.0573

Cited by 40 publications

(58 citation statements)

References 38 publications

(41 reference statements)

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“…Tensile tests were performed using an Instron 3365 materials testing machine (Instron, Bucks, UK) fitted with a 50 N load cell. A strain rate of 10 mm/min was used, similar to that previously reported in studies of human thoracic aorta (Duprey et al, 2010), mitral valve tissue (Kunzelman and Cochran, 1992) and porcine pulmonary valve tissue (Luo et al, 2014). The samples were hydrated using phosphate buffered saline solution (PBS; MP Biomedicals, LLC) maintained at 37°C.…”

Section: Biomechanical Performance Ii: Materials Properties (Uniaxial mentioning

confidence: 99%

“…Numerous types of detergents and biological agents have been used for decellularisation with varying concentrations, including SDS Luo et al, 2014), trypsin (Liao et al, 2008), EDTA (Liao et al, 2008;Paniagua Gutierrez et al, 2015), Triton X-100 (Liao et al, 2008) (Jordan et al, 2012) and hypertonic solutions (Jordan et al, 2012). It has previously been reported that decellularisation treatment can introduce changes in the tissue structure, which may influence the mechanical properties of the tissue Spina et al, 2003;Gilbert et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, to determine whether decellularised valves are suitable for clinical use, it is important to thoroughly assess functional performance including both the mechanical properties of the decellularised tissue and investigation of the performance of the decellularised valve under physiological flow conditions. Various test methods have been used to assess both the mechanical properties of valve tissue such as flexural (Engelmayr et al, 2005;Sacks et al, 2009b), local indentation (Cox et al, 2006), biaxial tensile (Sacks et al, 2009b;Billiar and Sacks, 2000;Fisher et al, 1986), uniaxial tensile (Luo et al, 2014;Korossis et al, 2002;Anssari-Benam et al, 2011), suture pull-out (Edwards et al, 2005;Walraevens et al, 2008), and dilation (Jennings et al, 2002;Korossis et al, 2005) as well as hydrodynamic performance of the valves under physiological flow conditions (Fisher et al, 1986;Jennings et al, 2002;Syedain et al, 2013;Reimer et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…A number of studies have investigated the effects of decellularisation on in vitro biomechanical and hydrodynamic properties of xenogeneic heart valves (Luo et al, 2014;Korossis et al, 2002;SchenkeLayland et al, 2003;Lichtenberg et al, 2006;Tudorache et al, 2007). The results from these studies are varied due to differences in the species, decellularisation protocol, test conditions and testing methodologies.…”

Section: Introductionmentioning

confidence: 99%

“…These studies have indicated that high concentration SDS decellularisation may induce changes in the mechanical properties of the tissue, as SDS can alter protein-protein interactions by opening the molecular structure of elastin and disturbing the bonding of collagen. Whereas, low concentration SDS based decellularisation treatment has shown favourable results for porcine aortic and pulmonary valves (Luo et al, 2014;Wilcox et al, 2005). Therefore, it was hypothesised that the low concentration SDS based treatment would minimally affect or maintain biomechanical and hydrodynamic properties of the human valves.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

In vitro biomechanical and hydrodynamic characterisation of decellularised human pulmonary and aortic roots

Desai

Vafaee

Rooney

et al. 2018

Journal of the Mechanical Behavior of Biomedical Materials

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A B S T R A C TBackground and purpose of the study: The use of decellularised biological heart valves in the replacement of damaged heart valves offers a promising solution to reduce the degradation issues associated with existing cryopreserved allografts. The purpose of this study was to assess the effect of low concentration sodium dodecyl sulphate decellularisation on the in vitro biomechanical and hydrodynamic properties of cryopreserved human aortic and pulmonary roots. Method: The biomechanical and hydrodynamic properties of cryopreserved decellularised human aortic and pulmonary roots were fully characterised and compared to cellular human aortic and pulmonary roots in an unpaired study. Following review of these results, a further study was performed to investigate the influence of a specific processing step during the decellularisation protocol ('scraping') in a paired comparison, and to improve the method of the closed valve competency test by incorporating a more physiological boundary condition. Results: The majority of the biomechanical and hydrodynamic characteristics of the decellularised aortic and pulmonary roots were similar compared to their cellular counterparts. However, several differences were noted, particularly in the functional biomechanical parameters of the pulmonary roots. However, in the subsequent paired comparison of pulmonary roots with and without decellularisation, and when a more appropriate physiological test model was used, the functional biomechanical parameters for the decellularised pulmonary roots were similar to the cellular roots. Conclusion: Overall, the results demonstrated that the decellularised roots would be a potential choice for clinical application in heart valve replacement.

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Section: Biomechanical Performance Ii: Materials Properties (Uniaxial mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In vitro biomechanical and hydrodynamic characterisation of decellularised human pulmonary and aortic roots

Desai

Vafaee

Rooney

et al. 2018

Journal of the Mechanical Behavior of Biomedical Materials

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Advances in xenogeneic donor decellularized organs: A review on studies with sheep and porcine‐derived heart valves

Inal,

Avci,

Hassan

et al. 2024

Bioengineering & Transla Med

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Heart valve replacement surgeries are performed on patients suffering from abnormal heart valve function. In these operations, the problematic tissue is replaced with mechanical valves or with bioprosthetics that are being developed. The thrombotic effect of mechanical valves, reflecting the need for lifelong use of anticoagulation drugs, and the short‐lived nature of biological valves make these two types of valves problematic. In addition, they cannot adapt to the somatic growth of young patients. Although decellularized scaffolds have shown some promise, a successful translation has so far evaded. Although decellularized porcine xenografts have been extensively studied in the literature, they have several disadvantages, such as a propensity for calcification in the implant model, a risk of porcine endogenous retrovirus (PERV) infection, and a high xenoantigen density. As seen in clinical data, it is clear that there are biocompatibility problems in almost all studies. However, since decellularized sheep heart valves have not been tried in the clinic, a large data pool could not be established. This review compares and contrasts decellularized porcine and sheep xenografts for heart valve tissue engineering. It reveals that decellularized sheep heart valves can be an alternative to pigs in terms of biocompatibility. In addition, it highlights the potential advantages of bioinks derived from the decellularized extracellular matrix in 3D bioprinting technology, emphasizing that they can be a new alternative for the application. We also outline the future prospects of using sheep xenografts for heart valve tissue engineering.

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Decellularization of human donor aortic and pulmonary valved conduits using low concentration sodium dodecyl sulfate

Vafaee

Thomas

Desai

et al. 2017

J Tissue Eng Regen Med

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The clinical use of decellularized cardiac valve allografts is increasing. Long‐term data will be required to determine whether they outperform conventional cryopreserved allografts. Valves decellularized using different processes may show varied long‐term outcomes. It is therefore important to understand the effects of specific decellularization technologies on the characteristics of donor heart valves. Human cryopreserved aortic and pulmonary valved conduits were decellularized using hypotonic buffer, 0.1% (w/v) sodium dodecyl sulfate and nuclease digestion. The decellularized tissues were compared to cellular cryopreserved valve tissues using histology, immunohistochemistry, quantitation of total deoxyribose nucleic acid, collagen and glycosaminoglycan content, in vitro cytotoxicity assays, uniaxial tensile testing and subcutaneous implantation in mice. The decellularized tissues showed no histological evidence of cells or cell remnants and >97% deoxyribose nucleic acid removal in all regions (arterial wall, muscle, leaflet and junction). The decellularized tissues retained collagen IV and von Willebrand factor staining with some loss of fibronectin, laminin and chondroitin sulfate staining. There was an absence of major histocompatibility complex Class I staining in decellularized pulmonary valve tissues, with only residual staining in isolated areas of decellularized aortic valve tissues. The collagen content of the tissues was not decreased following decellularization however the glycosaminoglycan content was reduced. Only moderate changes in the maximum load to failure of the tissues were recorded postdecellularization. The decellularized tissues were noncytotoxic in vitro, and were biocompatible in vivo in a mouse subcutaneous implant model. The decellularization process will now be translated into a good manufacturing practices‐compatible process for donor cryopreserved valves with a view to future clinical use. Copyright © 2016 The Authors Tissue Engineering and Regenerative Medicine published by John Wiley & Sons, Ltd.

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Development and Characterization of Acellular Porcine Pulmonary Valve Scaffolds for Tissue Engineering

Cited by 40 publications

References 38 publications

In vitro biomechanical and hydrodynamic characterisation of decellularised human pulmonary and aortic roots

In vitro biomechanical and hydrodynamic characterisation of decellularised human pulmonary and aortic roots

Advances in xenogeneic donor decellularized organs: A review on studies with sheep and porcine‐derived heart valves

Decellularization of human donor aortic and pulmonary valved conduits using low concentration sodium dodecyl sulfate

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