Decellularization and oxidation process of bamboo stem enhance biodegradation and osteogenic differentiation

H, Aswathy S.; Mohan, Chandini C.; P.S, Unnikrishnan; Krishnan, Amit G; Nair, Manitha B.

doi:10.1016/j.msec.2020.111500

Cited by 20 publications

(17 citation statements)

References 31 publications

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“…As cellulose is not degraded naturally by the human body, it could be advantageous to use this scaffold in anatomical areas where scaffold collapse is often observed and requires reinforcement with metal wires [28,29]. In contrast, if a degradable scaffold is desired for implantation, there are several strategies discussed herein to achieve controlled degradation without the production of a toxic by-product [30,31]. Finally, as a sustainable resource, plant-derived scaffolds have the potential to reduce waste production, energy use, and pollution while saving time and promoting biodiversity.…”

Section: Vegetal Scaffolds Are New Players To the Broader Field Of Tissue Engineeringmentioning

confidence: 99%

“…Thus, optimization and alternative protocols have been explored (Table 1). Amazon sword [55], Anthurium [35], Anthurium (queen) [35], Apple interior [24,32,33,53,56], Asparagus [56], Bamboo [31,35], Basil [55], Broccoli stem [52,56], Cabbage [57], Calathea zebrina [35], Carrot [52,56], Celery [53,56,58], Cucumber [56], Ficus hispida [59], Garcinia [59], Green onion [56,60], Impatiens capensis [35], Jujube [52], Leek [61], Lucky bamboo [55], Orchid pseudobulb [35], Pachira aquatica [59], Parsley stem [34,58], Peanut hairy root [34], Persimmon [52], Potato [56], Solenostemon "wasabi" [35], Spinach [34,54,55,58,60,62,63], Sweet yellow bell pepper [52], Sweet wormwood …”

Section: Alternative Strategies To Current Chemical Decellularization Protocolmentioning

confidence: 99%

“…For example, the vegetal kingdom provides highly diversified material, such as leaves, whose stiffness can vary considerably [95]. Interestingly, many studies showed that once decellularized, the leaf stiffness, as measured by Young's modulus (YM), decreases drastically [34,55] (Figure 6) and can reach, for some plant species, the same range of most of human tissues [31,35,53,55,61,64] such as soft organs (1-20 kPa), muscle (10 kPa), pre-calcified bone (100 kPa), or calcified cortical bone (20 GPa) [96] (Figure 7). For example, using atomic force microscopy (AFM), the local elasticity of apple hypanthium or succulent plant leaves was found to be ~1 kPa, which is similar to that found in brain tissue.…”

Section: Decellularized Plant Tissues Exhibit a Wide Range Of Mechanical Properties Which Can Be Matched To A Human Anatomical Sitementioning

confidence: 99%

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The Emerging Role of Decellularized Plant-Based Scaffolds as a New Biomaterial

Harris

Lacombe

Zenhausern

2021

IJMS

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The decellularization of plant-based biomaterials to generate tissue-engineered substitutes or in vitro cellular models has significantly increased in recent years. These vegetal tissues can be sourced from plant leaves and stems or fruits and vegetables, making them a low-cost, accessible, and sustainable resource from which to generate three-dimensional scaffolds. Each construct is distinct, representing a wide range of architectural and mechanical properties as well as innate vasculature networks. Based on the rapid rise in interest, this review aims to detail the current state of the art and presents the future challenges and perspectives of these unique biomaterials. First, we consider the different existing decellularization techniques, including chemical, detergent-free, enzymatic, and supercritical fluid approaches that are used to generate such scaffolds and examine how these protocols can be selected based on plant cellularity. We next examine strategies for cell seeding onto the plant-derived constructs and the importance of the different functionalization methods used to assist in cell adhesion and promote cell viability. Finally, we discuss how their structural features, such as inherent vasculature, porosity, morphology, and mechanical properties (i.e., stiffness, elasticity, etc.) position plant-based scaffolds as a unique biomaterial and drive their use for specific downstream applications. The main challenges in the field are presented throughout the discussion, and future directions are proposed to help improve the development and use of vegetal constructs in biomedical research.

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Section: Vegetal Scaffolds Are New Players To the Broader Field Of Tissue Engineeringmentioning

confidence: 99%

Section: Alternative Strategies To Current Chemical Decellularization Protocolmentioning

confidence: 99%

Section: Decellularized Plant Tissues Exhibit a Wide Range Of Mechanical Properties Which Can Be Matched To A Human Anatomical Sitementioning

confidence: 99%

See 1 more Smart Citation

The Emerging Role of Decellularized Plant-Based Scaffolds as a New Biomaterial

Harris

Lacombe

Zenhausern

2021

IJMS

View full text Add to dashboard Cite

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“…At the same time, the oxidized cellulose is conducive to protein adsorption, thus promoting cell adhesion and its function. This may be due to the covalent bond between the amino group in the protein and the aldehyde group on the surface of the fiber ( S H et al, 2021 ).…”

Section: Current Obstacles To the Application Of Plant Ecmmentioning

confidence: 99%

“…The oxidized scaffold may attract more macrophages due to its high hydrophilicity and amorphous properties. Macrophages secrete vascular endothelial growth factor, fibroblast growth factor, and platelet-derived growth factor, and initiate the process of vascular germination, anastomosis, and maturation, thus vascular formation can be seen around the scaffold, indicating its ability to facilitate the natural process of regeneration ( S H et al, 2021 ). Modulevsky et al implanted the cellulose scaffolds obtained from decellularized apples (proteins completely removed) under mice skin, and histological analysis revealed a typical foreign body reaction to the scaffold at 1 week.…”

Section: Current Obstacles To the Application Of Plant Ecmmentioning

confidence: 99%

Current Advances in the Development of Decellularized Plant Extracellular Matrix

Zhu

Zhang

Wang

et al. 2021

Front. Bioeng. Biotechnol.

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An imbalance exists between the supply of organs for transplantation and the number of patients in the donor transplant waiting lists. Current use of autologous, synthetic, and animal-derived grafts for tissue replacement is limited by the low availability, poor biocompatibility, and high cost. Decellularized plant scaffolds with remarkable physical similarities to human organs have recently emerged and have been found to present favorable characteristics that make them suitable as an alternative biomaterial, such as a superficial surface area, excellent water transport and retention, pre-existing vascular networks, interconnected porosity, and a wide range of mechanical properties. In addition to their unique and superior biocompatibility, plant-derived scaffolds present the advantages of low production cost, no ethical or supply constraints, simple operation and suitability for large-scale production and research. However, there are still some problems and deficiencies in this field, such as immature decellularization standards and methods, insufficient research on the biocompatibility of plant extracellular matrix. At present, research on decellularized plant extracellular matrix is still in its infancy, and its applicability to tissue engineering needs to be further improved. In this review, the current research progress on decellularized plant scaffolds is reviewed, the problems to be solved and future research directions are discussed.

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The effect of chemical detergents on the decellularization process of olive leaves for tissue engineering applications

Salehani

Rabbani

Biazar

et al. 2022

Engineering Reports

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The decellularization of plant tissues can be one of the design options of scaffolds in tissue engineering. Chemical detergents such as Triton X‐100 and sodium dodecyl sulfate (SDS) in different concentrations were used to decellularize olive leaves as an acellular plant matrix for tissue engineering. The samples were investigated by different analyses such as Hematoxylin and Eosin staining, SEM, tensile strength, swelling, water vapor transmission, and toxicity. The results of staining and toxicity tests showed that the Triton X‐100 decellularized samples at a concentration of 0.1% had the best morphology and the lowest toxicity. Mechanical results showed that the elasticity modulus of acellular samples was significantly reduced compared to normal leaf samples. While swelling rate and water vapor transmission in acellular samples compared to the control sample doubled and tripled, respectively. In general, acellular olive leaf can be suggested as a scaffold for tissue engineering applications.

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Decellularization and oxidation process of bamboo stem enhance biodegradation and osteogenic differentiation

Cited by 20 publications

References 31 publications

The Emerging Role of Decellularized Plant-Based Scaffolds as a New Biomaterial

The Emerging Role of Decellularized Plant-Based Scaffolds as a New Biomaterial

Current Advances in the Development of Decellularized Plant Extracellular Matrix

The effect of chemical detergents on the decellularization process of olive leaves for tissue engineering applications

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