De novo transcriptome sequencing in Frankliniella occidentalis to identify genes involved in plant virus transmission and insecticide resistance

Zhang, Zhijun; Zhang, Pengjun; Li, Weidi; Zhang, Jinming; Huang, Fang; Yang, Jian; Bei, Yi; Lu, Yitong

doi:10.1016/j.ygeno.2013.02.005

Cited by 65 publications

(52 citation statements)

References 46 publications

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“…Interestingly, among the differentially expressed genes, many genes related to cellular and humoral immune responses had higher transcript levels in the M population than in the TN1 population. Lysosomes and phagosomes have been shown to play a direct antiviral role [46]. In the M population, 38 genes (79.17%) involved in lysosome functions and 75 genes (85.23%) involved in phagosome functions had higher levels of transcripts compared to those in the TN1 population, strongly suggesting that these pathways were activated in the virulent M population.…”

Section: Resultsmentioning

confidence: 98%

Transcriptome Analysis of Fat Bodies from Two Brown Planthopper (Nilaparvata lugens) Populations with Different Virulence Levels in Rice

et al. 2014

PLoS ONE

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BackgroundThe brown planthopper (BPH), Nilaparvata lugens (Stål), one of the most serious rice insect pests in Asia, can quickly overcome rice resistance by evolving new virulent populations. The insect fat body plays essential roles in the life cycles of insects and in plant-insect interactions. However, whether differences in fat body transcriptomes exist between insect populations with different virulence levels and whether the transcriptomic differences are related to insect virulence remain largely unknown.Methodology/Principal FindingsIn this study, we performed transcriptome-wide analyses on the fat bodies of two BPH populations with different virulence levels in rice. The populations were derived from rice variety TN1 (TN1 population) and Mudgo (M population). In total, 33,776 and 32,332 unigenes from the fat bodies of TN1 and M populations, respectively, were generated using Illumina technology. Gene ontology annotations and Kyoto Encyclopedia of Genes and Genomes (KEGG) orthology classifications indicated that genes related to metabolism and immunity were significantly active in the fat bodies. In addition, a total of 339 unigenes showed homology to genes of yeast-like symbionts (YLSs) from 12 genera and endosymbiotic bacteria Wolbachia. A comparative analysis of the two transcriptomes generated 7,860 differentially expressed genes. GO annotations and enrichment analysis of KEGG pathways indicated these differentially expressed transcripts might be involved in metabolism and immunity. Finally, 105 differentially expressed genes from YLSs and Wolbachia were identified, genes which might be associated with the formation of different virulent populations.Conclusions/SignificanceThis study was the first to compare the fat-body transcriptomes of two BPH populations having different virulence traits and to find genes that may be related to this difference. Our findings provide a molecular resource for future investigations of fat bodies and will be useful in examining the interactions between the fat body and virulence variation in the BPH.

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Section: Resultsmentioning

confidence: 98%

Transcriptome Analysis of Fat Bodies from Two Brown Planthopper (Nilaparvata lugens) Populations with Different Virulence Levels in Rice

et al. 2014

PLoS ONE

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“…The KEGG database contains data from a systematic analysis of inner-cell metabolic pathways and functions of gene products, in addition to variants specific to particular organisms 30 . Pathway-based analyses can help to further understand the biological function and expression level of genes 14 .…”

Section: Resultsmentioning

confidence: 99%

De novo assembly and annotation of the Zhe-Maidong (Ophiopogon japonicus (L.f.) Ker-Gawl) transcriptome in different growth stages

Liu

Wang

et al. 2017

Sci Rep

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Zhe-Maidong (Ophiopogon japonicus (L.f.) Ker-Gawl) is a traditional medicinal herb in the family Liliaceae that has significant pharmacological effects on immunity and cardiovascular disease. In this study, three different growth stages of Zhe-Maidong were investigated using RNA-seq, and a total of 16.4 Gb of raw data was obtained. After filtering and assembling, 96,738 unigenes with an average length of 605.3 bp were ultimately generated. A total of 77,300 unigenes were annotated using information from five databases, including the NT, NR, SwissProt, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) databases. Additionally, the mechanisms of flavonoid, saponin and polysaccharide biosynthesis and of accumulation at different stages of tuber development were also characterized. From the first to third years, the contents of flavonoids, saponins and polysaccharides all increased, whereas the expression levels of related genes decreased in the flavonoid and saponin pathways and first increased and then decreased in the polysaccharide pathway. The results of this study provide the most comprehensive expressed sequence resource for Zhe-Maidong and will expand the available O. japonicus gene library and facilitate further genome-wide research and analyses of this species.

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“…A draft genome, and transcriptome and proteome data for whole body and different developmental stages of F. occidentalis are available, and differentially expressed (DE) genes in response to TSWV infection including antiviral defense are being annotated [66]. Global gene expression in response to TSWV infection identified DE genes in cellular processes and innate immune response [173]. Primary salivary gland transcriptome data identified genes involved in detoxification and inhibition of plant defense responses, and genes that may play a role in the extra-oral digestion of plant tissues and sugars [91].…”

Section: Experimental Approaches To Facilitate Further Insights Anmentioning

confidence: 99%

Plant Virus–Insect Vector Interactions: Current and Potential Future Research Directions

Dietzgen

Mann

Johnson

2016

Viruses

178

130

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Acquisition and transmission by an insect vector is central to the infection cycle of the majority of plant pathogenic viruses. Plant viruses can interact with their insect host in a variety of ways including both non-persistent and circulative transmission; in some cases, the latter involves virus replication in cells of the insect host. Replicating viruses can also elicit both innate and specific defense responses in the insect host. A consistent feature is that the interaction of the virus with its insect host/vector requires specific molecular interactions between virus and host, commonly via proteins. Understanding the interactions between plant viruses and their insect host can underpin approaches to protect plants from infection by interfering with virus uptake and transmission. Here, we provide a perspective focused on identifying novel approaches and research directions to facilitate control of plant viruses by better understanding and targeting virus–insect molecular interactions. We also draw parallels with molecular interactions in insect vectors of animal viruses, and consider technical advances for their control that may be more broadly applicable to plant virus vectors.

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De novo transcriptome sequencing in Frankliniella occidentalis to identify genes involved in plant virus transmission and insecticide resistance

Cited by 65 publications

References 46 publications

Transcriptome Analysis of Fat Bodies from Two Brown Planthopper (Nilaparvata lugens) Populations with Different Virulence Levels in Rice

Transcriptome Analysis of Fat Bodies from Two Brown Planthopper (Nilaparvata lugens) Populations with Different Virulence Levels in Rice

De novo assembly and annotation of the Zhe-Maidong (Ophiopogon japonicus (L.f.) Ker-Gawl) transcriptome in different growth stages

Plant Virus–Insect Vector Interactions: Current and Potential Future Research Directions

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