De novo transcriptome assembly of brackish water flea Diaphanosoma celebensis based on short-term cadmium and benzo[a]pyrene exposure experiments

Abstract

To develop a brackish water flea as a promising model for marine monitoring, Diaphanosoma celebensis were exposed to two pollutants, cadmium (Cd) and benzo[a]pyrene (BaP), which have different chemical characteristics and distinct modes of metabolic action on aquatic animals. Twenty-four hours after exposure to Cd (2 mg/L) or BaP (25 μg/L), whole body transcriptomes were analyzed. In total, 99.6 Mbp were assembled from nine libraries, resulting in 98,458 transcripts with an N50 of 1883 bp and an average contig length of 968 bp. Functional gene annotations were performed using Gene Ontology, Eukaryotic Orthologous Groups, and Kyoto Encyclopedia of Genes and Genomes pathway analyses. Cd significantly modulated endocrine and digestive enzyme system. Following BaP treatment, DNA repair and circadian rhythm related metabolisms were significantly modulated. Both the chemicals induced stress response and detoxification metabolism. This brackish water flea genomic information will be useful to monitor estuaries and coastal regions, as water fleas have been confirmed as promising sentinel models in freshwater ecosystems.Electronic supplementary materialThe online version of this article (10.1186/s41065-018-0075-3) contains supplementary material, which is available to authorized users.

Validation of Reference Genes for Quantitative Real-time PCR in Diaphanosoma Celebensis: For Chemical Exposure Conditions and Different Ages

“…In fact, in recent studies, the genome and transcriptome of D. celebensis were published 24,25 , leading to their potential use at the molecular level in marine ecotoxicology. However, studies on the validation of reference genes are not explored yet in this species.…”

Complete mitochondrial DNA of the marine water flea Diaphanosoma celebensis (Cladocera, Sididae)

“… 2019 ), environmental toxicology (Kim et al. 2018 ), and other aspects, as one of the non-model organisms in the marine and brackishwater environment. In genus Diaphanosoma , the complete mitochondrial genome of Diaphanosoma dubium has been firstly reported (Liu et al.…”

“…To date, more than 20 species are retrieved in the genus Diaphanosoma (http://v3.boldsystems.org/index.php/ TaxBrowser_Taxonpage?taxid=5262), whereas Korovchinsky (1989) and Korovchinsky (1993) were firstly to redescribe Diaphanosoma celebensis in tropical Asia. Also, D. celebensis has been used in aquaculture (Segawa and Yang 1987), developmental biology (In et al 2019), environmental toxicology (Kim et al 2018), and other aspects, as one of the non-model organisms in the marine and brackishwater environment. In genus Diaphanosoma, the complete mitochondrial genome of Diaphanosoma dubium has been firstly reported (Liu et al 2017), but no further studies on the complete mitochondrial genome have been reported from other Diaphanosoma spp.…”