De novo synthesis of enzyme-embedded covalent organic frameworks (COFs) using deep eutectic solvent: Pushing the COF limits

Regulating the Electron Structure of Covalent Organic Frameworks by Strong Electron‐Withdrawing Nitro to Construct Specific Li+ Oriented Channel

“…Covalent organic frameworks as a sort of new organic material have triggered keen interest in building ASEI on account of their permanent porosity, easily modified functionalized skeleton, and stronger mechanical strength. [35][36][37] Arumugam Manthiram, Kang, Qiao, Lu, etc. [38][39][40] synthesized and constructed the COF ASEI that equips with high mechanical strength.…”

Improving the Bioactivity and Stability of Embedded Enzymes by Covalent Organic Frameworks

“…As a kind of sister material of MOFs in reticular chemistry, COFs are connected by strong organic covalent bonds with higher stability, and more importantly, the metal-free nature of COFs offers an excellent opportunity to avert the effects of metal ions. − Several green methods have been reported for de novo constructing enzymes@COFs biocomposites. − The Chen group reported a universal water-based in situ assembly strategy for fabricating various enzymes@COFs, which can be easily scaled up (2.3 g per reaction) . The Ouyang group incorporated enzymes within COFs by a greener and more convenient mechanochemical encapsulation, and Kim de novo embedded enzymes in COFs by using a deep eutectic solvent as the solvent. , In addition, the catalytic performances of enzymes@COFs can be optimized via the intrinsic property of COFs or the interfacial interactions of enzymes and COFs. − However, the superiority of COFs in enhancing enzymatic activity, especially including their metal-free nature, has not been fully explored, which is vital for understanding and optimizing their catalytic performance.…”

“…55 The Ouyang group incorporated enzymes within COFs by a greener and more convenient mechanochemical encapsulation, and Kim de novo embedded enzymes in COFs by using a deep eutectic solvent as the solvent. 56,57 In addition, the catalytic performances of enzymes@COFs can be optimized via the intrinsic property of COFs or the interfacial interactions of enzymes and COFs. 58−63 However, the superiority of COFs in enhancing enzymatic activity, especially including their metal-free nature, has not been fully explored, which is vital for understanding and optimizing their catalytic performance.…”

Catechol-tetraethylenepentamine co-deposition modified cellulose filter paper for α-glucosidase immobilization and inhibitor screening from traditional Chinese medicine

“…This strategy not only stabilizes the enzyme structure fundamentally, but also enables continuous operation and rapid termination of enzymatic reactions. With the rapid development of materials science and technology, enzyme immobilization could be realized on various carrier materials, such as meta-organic frameworks (MOFs), 11 covalent organic frameworks (COFs), 12 carbon nanotubes (CNTs), 13 nanoflowers, 14 and other inorganic particles. However, for nanoscopic carriers, tedious operations such as centrifugation or filtration are required to separate the immobilized enzyme from the enzymatic reaction system due to their small size and low density, which are adverse to the termination of enzymatic reactions and the recycling use of the immobilized enzyme.…”