De novo leaf and root transcriptome analysis to explore biosynthetic pathway of Celangulin V in Celastrus angulatus maxim

Abstract

BackgroundCelastrus angulatus Maxim is a kind of crucial and traditional insecticidal plant widely distributed in the mountains of southwest China. Celangulin V is the efficient insecticidal sesquiterpenoid of C. angulatus and widely used in pest control in China, but the low yield and discontinuous supply impeded its further popularization and application. Fortunately, the development of synthetic biology provided an opportunity for sustainable supply of Celangulin V, for which understanding its biosynthetic pathway is indispensable.ResultsIn this study, six cDNA libraries were prepared from leaf and root of C. angulatus before global transcriptome analyses using the BGISEQ-500 platform. A total of 104,950 unigenes were finally obtained with an average length of 1200 bp in six transcriptome databases of C. angulatus, in which 51,817 unigenes classified into 25 KOG classifications, 39,866 unigenes categorized into 55 GO functional groups, and 48,810 unigenes assigned to 135 KEGG pathways, 145 of which were putative biosynthetic genes of sesquiterpenoid and triterpenoid. 16 unigenes were speculated to be related to Celangulin V biosynthesis. De novo assembled sequences were verified by Quantitative Real-Time PCR (qRT-PCR) analysis.ConclusionsThis study is the first report on transcriptome analysis of C. angulatus, and 16 unigenes probably involved in the biosynthesis of Celangulin V were finally collected. The transcriptome data will make great contributions to research for this specific insecticidal plant and the further gene mining for biosynthesis of Celangulin V and other sesquiterpene polyol esters.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5397-z) contains supplementary material, which is available to authorized users.

Transcriptome analysis of polycyclic sesquiterpene biosynthesis in Leonurus sibiricus L.

“…Furthermore, this transcriptome data may provide precise clues to trace other natural products biosynthesis processes in L. sibiricus. cDNA library production and BGISEQ-500 sequencing The isolation of poly (A) + mRNA, reverse transcription of double-strand cDNA and cDNA library production were carried out as previously described [32]. Each cDNA library was sequenced in a single lane of the BGISEQ-500 system according to the operational instructions at the Beijing Genomics Institute (BGI-Shenzhen, China).…”

“…In order to get perfect clean read data for de novo assembly, the raw reads from BGISEQ-500 were filtered and trimmed mainly using software Trinity (v2.0.6) [33], and software TGICL (v2.0.6) [34] as represented in our previous article [32].…”

Synthetic biology in China: Review and prospects

“…北京大学iGEM 团队用TFs设计了测定芳香烃污染物的传感器 [112] . 4种 [115] 、甜菊糖苷(steviolglycosides)(治疗记 忆障碍药物) [116] 、番茄红素(抗氧化保健食品) [117] , 香 叶醇江(食用香精) [118] 、苦皮藤素V(celangulin V)(蔬菜 作物驱虫剂) [119] 等; 黄酮类, 如松属素(pinocembrin)(局 部麻醉作用) [120] 、灯盏花素(breviscapine)(扩张脑血 管, 抗血凝作用) [121] 等; 植物甾体类, 如菜油甾醇(campesterol)(植物激素) [122] 、21-托皮质激素(21-deoxycortisol)(药用糖皮质激素的前体分子) [123] 、薯蓣皂苷元 (diosgenin)(甾体激素类药物的基础原料) [124] 等; 抗生 素及药物类, 如链霉素和衣霉素 [125] 、金红霉素 [126] , 尼 可霉素片段 [127] 、氨甲酰多聚草氨酸 [128] 、丹参酸A (salvianic acid A) [60] 、大麻素(cannabinoids) [129] 、羟基 酪醇(hydroxytyrosol) [130] 等. 有一些合成天然化合物的产量已经达到前所未 有的水平, 具有很强的工业价值.…”

Identification, expression analysis, and potential roles of microRNAs in the regulation of polysaccharide biosynthesis in Polygonatum cyrtonema Hua

“…The differential expression multiple of the gene between different samples was calculated based on the gene expression level (FPKM value). In our study, genes with FC ≥ 2.00 and false discovery rate (FDR) ≤ 0.001 by the Poisson Dis method were considered differentially expressed [39][40][41]. Based on the differential miRNA expression results, the pheatmap package of the R software was used for hierarchical clustering analysis.…”