De Novo Design of a Cyclic Polyhistidine Peptide for Binding with Quantum Dots: Self-Assembly Investigation Using Capillary Electrophoresis

Qiu, Lin; Zhang, Chencheng; Gu, Tao; Zhu, Zhilan; Wang, Jianpeng; Liu, Li; Ding, Shumin; Liu, Xiaoqian; Jiang, Pengju

doi:10.1007/s10337-017-3319-x

Cited by 4 publications

(5 citation statements)

References 29 publications

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“…The benefit of polyhistidine self‐assembly is that it uses tried‐and‐true procedures, without the need for extra QD surface modification, and gives considerable control over the resulting stoichiometry [82]. More advanced polyhistidine tags are now being developed, offering reduced steric obstruction during assembly with QD formation and greater binding affinity with biomolecules [83]. Few examples of quantum dots that are commonly utilized in flow cytometry are used QDs in multicolor flow cytometry are QD525, QD545, QD565, QD585, QD605, QD655, QD705, and QD800 [84].…”

Section: Cell Labelling Componentsmentioning

confidence: 99%

Flow cytometry: Aspects and application in plant and biological science

Jyoti,

Chandel,

Singh

2023

Journal of Biophotonics

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Flow cytometry is a potent method that enables the quick and concurrent investigation of several characteristics of single cells in solution. Photodiodes or photomultiplier tubes are employed to detect the dispersed and fluorescent light signals that are produced by the laser beam as it passes through the cells. Photodetectors transform the light signals produced by the laser into electrical impulses. A computer then analyses these electrical impulses to identify and measure the various cell populations depending on their fluorescence or light scattering characteristics. Based on their fluorescence or light scattering properties, cell populations can be examined and/or isolated. This review covers the basic principle, components, working and specific biological applications of flow cytometry, including studies on plant, cell and molecular biology and methods employed for data processing and interpretation as well as the potential future relevance of this methodology in light of retrospective analysis and recent advancements in flow cytometry.

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Section: Cell Labelling Componentsmentioning

confidence: 99%

Flow cytometry: Aspects and application in plant and biological science

Jyoti,

Chandel,

Singh

2023

Journal of Biophotonics

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“…The advantage of polyhistidine self-assembly is the fact that it does not require additional QD surface modification, employs well-established protocols and provides reasonable control over resulted stoichiometry [99]. Currently, the preparation of more sophisticated polyhistidine tags is carried out providing less steric hindrance during assembly with QDs and better binding affinity with biomolecules [100].…”

Section: Fluorescent Label Conjugated Antibodiesmentioning

confidence: 99%

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Voronin

Kozlova

Verkhovskii

et al. 2020

IJMS

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Flow cytometry nowadays is among the main working instruments in modern biology paving the way for clinics to provide early, quick, and reliable diagnostics of many blood-related diseases. The major problem for clinical applications is the detection of rare pathogenic objects in patient blood. These objects can be circulating tumor cells, very rare during the early stages of cancer development, various microorganisms and parasites in the blood during acute blood infections. All of these rare diagnostic objects can be detected and identified very rapidly to save a patient’s life. This review outlines the main techniques of visualization of rare objects in the blood flow, methods for extraction of such objects from the blood flow for further investigations and new approaches to identify the objects automatically with the modern deep learning methods.

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“…Interestingly, P1-P5 had no, or a very low, proportion of His residue, implying a different binding mechanism to that of previously reported His-rich QD-binding peptides. 23,24 To conrm the peptide binding activity to the QDs, the binding assay was carried out using a peptide array. Fig.…”

Section: Screening Of Cdte/cds Qd-binding Peptidesmentioning

confidence: 99%

“…21,22 So far, a few peptides have been reported to possess the capacity to bind to QDs based on histidine (His or H)-metal affinity. 23,24 These His-rich peptides have the potential to bind to QD surfaces; however, they are limited to ZnS-shelled QDs. 23,24 Since CdTe/CdS core/shell QDs with high quantum yield can be aqueously synthesised, this QD type is one of the widely used QDs for biological uorescence probes.…”

Section: Introductionmentioning

confidence: 99%

“…23,24 These His-rich peptides have the potential to bind to QD surfaces; however, they are limited to ZnS-shelled QDs. 23,24 Since CdTe/CdS core/shell QDs with high quantum yield can be aqueously synthesised, this QD type is one of the widely used QDs for biological uorescence probes. 25 Despite its potent material, no peptide binders to CdTe/CdS QDs have been reported up to date.…”

Section: Introductionmentioning

confidence: 99%

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