De Novo Assembly and Analysis of the White-Backed Planthopper (Sogatella furcifera) Transcriptome

Abstract

The white-backed planthopper Sogatella furcifera (Horváth) has become an important pest on rice in China and Southeast Asian countries. White-backed planthopper wing bud length is in relation to adult wing length, but little is known about the development and differentiation of wing buds at the molecular level. Using Illumina HiSeq high-throughput sequencing technology, we sequenced four cDNA libraries, two biological replicates of long-winged female fifth-instar nymphs (LW), and two of short-winged nymphs (SW). In total, 62,154 unigenes with an average length of 984 bp and N50 length of 1,878 bp were obtained by de novo transcriptome assembly. A total of 18,416 open reading frames (ORFs) were predicted based on the unigenes. Ninety-three percentage of these ORFs could be annotated by searching for homology in six protein databases. A total of 184 differentially expressed genes (DEGs) with 129 upregulated and 55 downregulated were found in SW compared to LW. Gene Ontology and euKaryotic Orthologous Group classification provided comprehensive information about the function of each gene. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed five enriched pathways including three metabolic pathways. In addition, we found that some DEGs were relevant to muscle movement and cuticle and likely involved in development and differentiation of wing buds. This study provided transcriptome resource of female fifth-instar nymphs of white-backed planthopper including long-winged and short-winged nymphs, and different molecular features between them lay the foundation for adult wing morph prediction, promoting further studies on planthopper population management.

Comparative transcriptional analysis and identification of hub genes associated with wing differentiation of male in Aphis gossypii

“…8). These signaling pathways were also reported signi cantly up-regulated in alate brown citrus aphid Toxoptera citricida and in long winged Sogatella furcifera (Shang et al, 2016;Liang et al, 2018). Moreover, silencing of lipid synthesis and degradation genes in T. citrida leading to underdeveloped wings, which revealed that both lipid metabolism and energy supply are essential for aphid wing bud differentiation (Shang et al, 2016;Liang et al, 2018).…”

SMRT sequencing of the full-length transcriptome of the white-backed planthopper Sogatella furcifera

“…To date, only a few full-length cDNA sequences have been reported in S. furcifera. Recently, Liang et al (2018) used second-generation sequencing technology to sequence the transcriptome of S. furcifera and reported that the unigene length was 200-400 bp, accounting for 41.50% of the total number of genes, whereas those longer than 4,000 bp only accounted for 3.37%. Furthermore, 18,416 ORFs were generated based on the unigenes.…”

Peer Review #3 of "SMRT sequencing of the full-length transcriptome of the white-backed planthopper Sogatella furcifera (v0.1)"

“…Furthermore, 18,416 ORFs were generated based on the unigenes. In addition, the generated ORFs were compared with those in the Nr, Swiss-Prot, GO, COG, and KEGG databases, and 18,415 transcripts were annotated (Liang et al 2018). In the present study, we used the PacBio SMRT sequencing platform to obtain 100,360 SSRs and 121,395 CDSs, of which 48,873 carried complete ORFs.…”