DDX46 silencing inhibits cell proliferation by activating apoptosis and autophagy in cutaneous squamous cell carcinoma

Abstract

dead-Box Helicase 46 (ddX46) is an aTP-dependent rna helicase that plays a central role in transcription splicing and ribosome assembly. However, the role of ddX46 in cutaneous squamous cell carcinoma (cScc) remains to be elucidated. The aim of the present study was to investigate the role of ddX46 in cScc by assessing ddX46 expression levels in cScc tissues and cell lines. The effect of ddX46 silencing on cScc cell proliferation, apoptosis and autophagy were also analyzed. it was demonstrated that ddX46 was significantly overexpressed in cScc tissues and cells (P<0.05). Furthermore, it was found that ddX46 silencing could dramatically inhibit cell proliferation (P<0.05). Moreover, cell apoptosis and autophagy were activated in ddX46 silencing groups (P<0.05). Therefore, the present results suggested that ddX46 was overexpressed in cScc and that ddX46 silencing can inhibit cell proliferation by inducing apoptosis and activating autophagy. Thus, ddX46 may serve as a novel potential therapeutic target for cScc.

The role of DDX46 in breast cancer proliferation and invasiveness: A potential therapeutic target

“…Based on its involvement in the pathogenesis of breast cancer, the epithelium, and its downregulation significantly inhibited the proliferation of CRC cells (Li et al, 2015). Furthermore, DDX46 silencing in esophageal squamous cell carcinoma, cutaneous squamous cell carcinoma and gastric cancer cells also restrains their proliferative capacity (Chen et al, 2021;Li et al, 2016;Lin et al, 2020). Our breast and colon cancers (Greco et al, 2001;Malaterre et al, 2007;Zorbas et al, 1999).…”

“…Li et al reported that DDX46 protein is overexpressed in colorectal cancer (CRC) tissues compared to the adjacent normal colon epithelium, and its downregulation significantly inhibited the proliferation of CRC cells (Li et al, 2015). Furthermore, DDX46 silencing in esophageal squamous cell carcinoma, cutaneous squamous cell carcinoma and gastric cancer cells also restrains their proliferative capacity (Chen et al, 2021; Li et al, 2016; Lin et al, 2020). Our findings further suggested that DDX46 plays an essential role in the proliferation and invasion of breast cancer cells.…”

PRR14 acts a novel oncogene activating the PI3K signal pathway in human cutaneous squamous cell carcinoma

“…In recent years, it has been found that a variety of novel molecules are involved in the occurrence and development of cSCC and as functions of promoting tumor formation through a variety of signaling pathways, such as apurinic/apyrimidinic endonuclease 1 (APE1) 22 , DEAD-box helicase 46 (DDX46) 23 , targeting protein for Xenopus kinesin-like protein 2 (TPX2) 24 , kynureninase (KYNU) 25 , signal transducer and activator of transcription 3 (STAT3) 26 , histone deacetylase 3 (HDAC3) 27 , SAM- and SH3-domain containing 1 gene (SASH1) 28 , karyopherin subunit alpha 4 (KPNA4) 29 , ephrin B receptor 2 (EphB2) 30 , and cell division cycle 20 (CDC20) 31 . APE1 has been reported to be significant high regulated in tumor tissues of human cSCC patients, and its high expression promoted the proliferation and migration of cSCC cells 22 .…”

Integrated Bioinformatics Analysis Reveals Marker Genes and Potential Therapeutic Targets for Pulmonary Arterial Hypertension

“…The abnormal expression of DDX46 has also been reported in tumors [ 44 , 45 , 46 ]. The expression of DDX46 is increased in esophageal squamous cell carcinoma and colon cancer tissues, promoting cell growth and inhibiting apoptosis [ 44 , 46 , 47 ]. DDX46 is abnormally expressed in osteosarcoma tissues and cells, promoting cell growth and invasion [ 48 ].…”