DD genotype of ace gene I/D polymorphism is associated in a turkish study population with osteoarthritis

Abstract

This study was conducted in Turkish osteoarthritis patients to determine the frequency of I/D polymorphism genotypes of angiotensin converting enzyme gene, and to examine the role of this polymorphism in osteoarthritis development. Genomic DNA obtained from 200 persons (135 patients with osteoarthritis and 65 healthy controls) was used in the study. DNA was multiplied by polymerase chain reaction using I and D allele-specific primers. Polymerase chain reaction products were assessed with CCD camera by being exposed to 2% agarose gel electrophoresis. There was statistically significant difference between the groups with respect to genotype distribution (P < 0.001). The D allele frequency was indicated as 69% and I allele was as 31% in the patients, whereas it was 55-45% in the control group. Consequently, in this study, we may assert that ACE gene I/D polymorphism DD genotype determination is significant criteria for identifying patients who are likely to develop osteoarthritis in east population of Turkey.

Lack of association between plasminogen activator inhibitor type-1 (PAI-1) gene 4G/5G polymorphism and osteoarthritis

“…Control subjects were consecutively selected among people without personal and family history of OA. The clinical characteristics of patients with OA and controls were summarized in Table 1 [13].…”

Angiotensin-converting enzyme insertion/deletion polymorphism associated with allergic rhinitis susceptibility: Evidence from 1410 subjects

“…32,33 Up to now, several studies on the association between ACE I/D polymorphism and atopic disorders have been reported. [34][35][36] The mechanism underlying why the ACE I/D polymorphism affects susceptibility to atopic disorders is undetermined. There is ample evidence indicating that the ACE I/D polymorphism may modulate the expression of the ACE gene and the DD and D allele of the ACE gene which brings about higher plasma ACE levels and increased angiotensin II levels which are associated with many atopic disorders such as rheumatoid arthritis, asthma and nephrotic syndrome.…”

Evidence of Angiotensin Converting Enzyme (Ace) Insertion Polymorphism in Rheumatoid Arthritis From Pakistani Patients

“…A large difference has been reported in the usual circulation of ACE gene alleles in different populations. The frequency of D allele in normal Caucasians is 50-58%, but 35-39% in normal Chinese [20][21][22]. The renin-angiotensin system plays an key role in maintaining body fluid and sodium balance and has been involved in a many complex disorders [23][24][25].…”

“…Gunes, et al [32] also did not found any difference in ACE gene I/D polymorphism genotypes in hypertensive patients and healthy controls in the Turkis population. Bayram, et al [22] reported that DD homozygotes was found to be significantly higher in osteoarthritis than in healthy controls (DD) and D allele frequency and DD homozygosis were found to be significantly higher in polycystic ovary syndrome patients than in controls in the Turkish population. ACE gene also considered as a top candidate gene for cardiovascular studies as well [33].…”