Lack of association between plasminogen activator inhibitor type-1 (PAI-1) gene 4G/5G polymorphism and osteoarthritis

Abstract

This study was conducted in Turkish osteoarthritis patients to determine the frequency of 4G/5G polymorphism genotypes of plasminogen activator inhibitor type-1 gene and to examine the role of this polymorphism in osteoarthritis development. Genomic DNA obtained from 200 persons (140 patients with osteoarthritis and 60 healthy controls) was used in the study. DNA was amplified by polymerase chain reaction using 4G allele- and 5G allele-specific primers. Polymerase chain reaction products were assessed with CCD camera by being exposed to 2% agarose gel electrophoresis. No statistically significant difference between the groups with respect to genotype distribution was found (P > 0.05) in the study. The 4G allele frequency was indicated as 44% and 5G allele was as 56% in patients, whereas this was 45-55% in the control group. This study has established that 4G/5G polymorphism genotypes of plasminogen activator inhibitor type-1 gene do not play a role in the development of osteoarthritis in the Turkish population.

The Association Between Development and Progression of Multinodular Goiter and Thyroid-Stimulating Hormone Receptor Gene D727E and P52T Polymorphisms

“…The study of Cuddihy et al (1996) also supports the relationship between Grave's disease and the TSHR gene P52T polymorphism. This inconsistency in the results can be explained by the fact that the diversities in gene pools, lifestyle, and gene-environment interactions may not be reflected to the genotypes of each population in a similar manner (Cosan et al, 2009;Gunes et al, 2010;Bayram et al, 2012). Although MNG has a very high frequency in female individuals than in male individuals, no differences have been found between the genders in terms of the genotype distribution of the TSHR gene D727E and P52T polymorphisms.…”

Relationship between plasminogen activator inhibitor type-1 (PAI-1) gene polymorphisms and osteoporosis in Turkish women

“…A relationship between the PAI-1 4G/5G gene polymorphism and diseases, such as coronary artery disease, hypertension, stroke, and obesity, has been reported previously, but this polymorphism is not related to asthma, rheumatoid arthritis, and osteoarthritis (15)-(21). Genetic variations occur in populations.…”

“…The correlation of the PAI-1 4G/5G insertion/deletion polymorphism with several diseases, such as coronary artery disease, asthma, hypertension, stroke, obesity, rheumatoid arthritis, and osteoarthritis, has been investigated previously (15)-(21). However, the contribution of PAI-1 insertion/deletion variations (4G/5G) to osteoporosis has not been investigated in the Turkish population.…”