DcTT8, a bHLH transcription factor, regulates anthocyanin biosynthesis in Dendrobium candidum

Jia, Ning; Wang, Jingjing; Liu, Jiameng; Jiang, Jinlan; Sun, Jing; Yan, Peipei; Sun, Yufeng; Wan, P.J.; Ye, Wei; Fan, Bin

doi:10.1016/j.plaphy.2021.03.006

Cited by 30 publications

(21 citation statements)

References 45 publications

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“…BcTT8 -silenced non-heading Chinese cabbage showed a notable downregulation of anthocyanin biosynthetic genes BcDFR , BcLODX , and BcUF3GT , while the transcription level of BcFLS increased considerably ( Figure 7 C). Previous studies had proved that in other plant species, bHLH transcription factors could activate the expression of DFR , ANS, and UFGT , which improve the anthocyanin content [ 16 , 17 ]; thus, we proposed that in non-heading Chinese cabbage, transcription factor BcTT8 also facilitates the similar function, which could explain the downregulation of the LBGs and the decrease in anthocyanin production. We should mention that the production of flavonols and anthocyanins share the same biosynthesis pathway and compete for the same precursors.…”

Section: Discussionmentioning

confidence: 89%

“…The bHLH proteins influence anthocyanin synthesis by directly activating the anthocyanin pathway genes or forming the MBW complex to mediate the structural genes. It was reported that DcTT8 could regulate anthocyanin in Dendrobium candidum through inducing the expression of DcF3′H and DcUFGT [ 16 ], and the same activation pattern was also observed in other higher plants such as Nelumbo nucifera and tree peony ( Paeonia suffruticosa ) [ 17 , 18 ]. What is more, in Arabidopsis , MBW complexes could directly target AtDFR and AtLODX to regulate their transcriptional activity and in Medicago truncatula MtTT8 together with MtWD40-1, they could directly target activated promoters of MtANS to regulate anthocyanin synthesis [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 81%

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Comparative Transcriptome Analysis of Purple and Green Non-Heading Chinese Cabbage and Function Analyses of BcTT8 Gene

Tang

Xiao

Yin

et al. 2022

Genes

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Non-heading Chinese cabbage (Brassica campestris ssp. chinensis) is an important vegetative crop in the south of China. As an antioxidant, anthocyanin is the major quality trait for vegetables with purple leaves or petioles. However, the molecular biosynthetic mechanism of anthocyanin in non-heading Chinese cabbage has not been explained exclusively. In this study, two non-heading Chinese cabbage with contrasting colors in the leaves were used as the materials for RNA-seq. A total of 906 DEGs were detected, and we found that the anthocyanin and flavonoid biosynthetic pathways are significantly enriched in the purple NHCC. The transcriptome result was verified by RT-qPCR. Though bioinformatics analysis, BcTT8 was selected as the candidate gene for the regulation of anthocyanin synthesis, and the characterization of BcTT8 was elucidated by the functional analyses. The results proved that BcTT8 is a nucleus protein and phylogenetically close to the TT8 protein from Brassica. After silencing BcTT8, the total anthocyanin content of pTY-BcTT8 plants decreased by 42.5%, and the relative expression levels of anthocyanin pathway genes BcDFR, BcLODX and BcUF3GT-1 were significantly downregulated, while the transcription level of BcFLS was significantly upregulated. Compared with the wild type, the transgenic Arabidopsis showed obvious violet in the cotyledons part, and the anthocyanin biosynthetic genes such as AtDFR and AtLODX were significantly upregulated. In conclusion, BcTT8 is critical in the anthocyanin synthesis process of non-heading Chinese cabbage. Our findings illustrated the molecular mechanism of anthocyanin biosynthesis in non-heading Chinese cabbage.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 81%

Comparative Transcriptome Analysis of Purple and Green Non-Heading Chinese Cabbage and Function Analyses of BcTT8 Gene

Tang

Xiao

Yin

et al. 2022

Genes

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“…The bHLH TFs are important members of the plant gene regulatory network. In addition to participating in the growth and development and responding to adversity stress, bHLH TFs can also play a role in the process of biosynthesis (Jia et al, 2021 ; Singh et al, 2021 ). To identify and analyze S. cusia at the genome-wide level, we downloaded the protein sequence of bHLHs from Arabidopsis thaliana ( https://www.arabidopsis.org/browse/genefamily/bHLH.jsp ).…”

Section: Methodsmentioning

confidence: 99%

High-Quality Genome of the Medicinal Plant Strobilanthes cusia Provides Insights Into the Biosynthesis of Indole Alkaloids

Ning

et al. 2021

Front. Plant Sci.

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Strobilanthes cusia (Nees) Kuntze is an important plant used to process the traditional Chinese herbal medicines “Qingdai” and “Nanbanlangen”. The key active ingredients are indole alkaloids (IAs) that exert antibacterial, antiviral, and antitumor pharmacological activities and serve as natural dyes. We assembled the S. cusia genome at the chromosome level through combined PacBio circular consensus sequencing (CCS) and Hi-C sequencing data. Hi-C data revealed a draft genome size of 913.74 Mb, with 904.18 Mb contigs anchored into 16 pseudo-chromosomes. Contig N50 and scaffold N50 were 35.59 and 68.44 Mb, respectively. Of the 32,974 predicted protein-coding genes, 96.52% were functionally annotated in public databases. We predicted 675.66 Mb repetitive sequences, 47.08% of sequences were long terminal repeat (LTR) retrotransposons. Moreover, 983 Strobilanthes-specific genes (SSGs) were identified for the first time, accounting for ~2.98% of all protein-coding genes. Further, 245 putative centromeric and 29 putative telomeric fragments were identified. The transcriptome analysis identified 2,975 differentially expressed genes (DEGs) enriched in phenylpropanoid, flavonoid, and triterpenoid biosynthesis. This systematic characterization of key enzyme-coding genes associated with the IA pathway and basic helix-loop-helix (bHLH) transcription factor family formed a network from the shikimate pathway to the indole alkaloid synthesis pathway in S. cusia. The high-quality S. cusia genome presented herein is an essential resource for the traditional Chinese medicine genomics studies and understanding the genetic underpinning of IA biosynthesis.

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“…Anthocyanin composition in green, lilac and purple leaves were collected based on UPLC-MS/MS following the method described in Jia et al (2021) (Jia et al, 2021a;Jia et al, 2021b). In brief, green, lavender, and purple leaves are ground into a fine powder with liquid nitrogen.…”

Section: Flavonoid Content Determinationmentioning

confidence: 99%

Combination of Single-Molecule Long-Read Sequencing and Illumina Sequencing Revealed the Mechanism of Anthocyanins Accumulation in an Ornamental Grass, Pennisetum Setaceum ‘Rubrum’

Liu

Teng

Fan

et al. 2021

Preprint

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Pennisetum setaceum ‘Rubrum’ is an ornamental herb with purple leaves, and it is widely used in the construction of landscaping. However, the current next generation sequencing (NGS) transcriptome information is not satisfactory mainly because of the enormous difficulty in obtaining full-length transcripts. What’s more, the molecular mechanisms of anthocyanin accumulation have not been thoroughly studied. In this study, we used PacBio full-length transcriptome sequencing combined with NGS sequencing technology to conduct transcriptome analysis on leaves showing different colors at different stages to clarify the molecular mechanism involved in the color change of P. setaceum ‘Rubrum’. A total of 280,413 full-length non-chimeric reads (FLNC) sequences were obtained based on single-molecule long-read sequencing technology. We obtained 140,633 high quality (HQ) transcripts and 2,683 low quality (LQ) transcripts and identified 5,352 alternative splicing (AS). In addition, a total of 93,066 ORFs, including 57,457 full open links and 2,910 lncRNA sequences were screened out. Furthermore, a total of 10,795 differentially expressed genes were identified. Gene ontology (GO) cluster and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed the underlying mechanism of anthocyanin accumulation. In this study, to our best knowledge, we provided the full-length transcriptome information of P. setaceum ‘Rubrum’ for the first time. The underlying mechanism of anthocyanin accumulation in P. setaceum ‘Rubrum’ was further discussed based on the newly generated transcriptome data. The information will not only facilitate the gene function studies but also pave the way for future breeding projects of Pennisetum setaceum .

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DcTT8, a bHLH transcription factor, regulates anthocyanin biosynthesis in Dendrobium candidum

Cited by 30 publications

References 45 publications

Comparative Transcriptome Analysis of Purple and Green Non-Heading Chinese Cabbage and Function Analyses of BcTT8 Gene

Comparative Transcriptome Analysis of Purple and Green Non-Heading Chinese Cabbage and Function Analyses of BcTT8 Gene

High-Quality Genome of the Medicinal Plant Strobilanthes cusia Provides Insights Into the Biosynthesis of Indole Alkaloids

Combination of Single-Molecule Long-Read Sequencing and Illumina Sequencing Revealed the Mechanism of Anthocyanins Accumulation in an Ornamental Grass, Pennisetum Setaceum ‘Rubrum’

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