Daya Hidup Spermatozoa Epididimis Kambing Peranakan Ettawa yang Dipreservasi dengan Pengencer Tris dan Bberbagai Konsentrasi Maltosa

Abstract

Cauda epididymal spermatozoa could be used as an alternative source of gamete in the application of various reproductive technologies, since the spermatozoa is motile and has ability for fertilizing the oocyte. Theobjective of this research was to examine the effectivity of maltose in maintaining viability of ettawa crossbreed goat epididymal spermatozoa preserved at 3–5oC. Five testis with epididymides of ettawa crossbreed goat were obtained from slaughterhouse. Epididymal spermatozoa was collected by the combination of slicing, flushing and tissues pressure of cauda epididymides with physiological saline (0.9% NaCl). Collected-spermatozoa wasdivided in equal volume into three tubes and diluted with Tris extender containing 20% egg yolk (control), Tris extender + 0.3 g maltose/100 ml (M0.3), and Tris extender + 0.6 g maltose/100 ml (M0.6), respectively. Dilutedspermatozoa was stored in refrigerator at 3–5oC. Quality of diluted-spermatozoa including percentages of motile spermatozoa (MS) and live spermatozoa (LS) were evaluated every day during storage at 3–5oC for four days. Data were analyzed using completely randomized design with three treatments and five replicates. Means were compared significant difference test at 0.05 significant level. Results of this study showed that mean spermatozoaconcentration, percentage of MS, percentage of LS, and percentage of abnormal spermatozoa of ettawa crossbreed goat fresh epididymal spermatozoa were 3,220 million cell/ml, 70%, 81%, and 4.3%, respectively. At day-5 of storage, percentages of MS and LS for M0.3 (38 and 60.4%) and M0.6 (38 and 57.2%) were significantly (P<0.05) higher than control (32 and 55.4%). In conclusion, addition of 0.3 and 0.6% maltose in Tris extender could be maintained viability of ettawa crossbreed goat epididymal spermatozoa preserved at 3–5oC forthree days.

Addition of Sugarcane Juice to Tris Egg Yolk Buffer Diluent Improves the Quality of the Stored Diluted Semen of Kacang Goats at 5°C

“…However, semen volume in this study was lower than that reported by three previous studies, which were 1.54±0.32 ml , 2.64 ml (Pratiwi, 2007) and 2.44 ml (Armansyah et al, 2018). The reported value of the semen volume is still within the normal range of goat semen as written by some researchers, such as 0.96 ml/ejaculate (Putranti et al,2020), 0.70-1.50 ml (Suyadi et al, 2004), 0.640 (Rizal M., 2009) and 0.80 ml (Pamungkas et al, 2008). Different volumes among studies would be affected by the number of factors such as the frequency of ejaculation and variance of individual livestock breeds, age, season, libido, and the variation in the condition of livestock (LukmanHy et al, 2022b).…”

Benefits of Nori Fruit Extract (Morinda Citrifolia Linn) in Increasing the Storage of Spermatozoa Y Bali Cows Resulting from Sexing at a Temperature of 5oC

“…What is meant by progressive motility is motility that moves nimbly, moves forward, can penetrate the ovum for fertilization, and also experiences a decrease because during the semen processing process there will be a decrease in sperm quality including motility. According to Rizal (2009), semen reservation can cause the death of spermatozoa, due to damage to the spermatozoa plasma membrane due to lipid/fat peroxidation and during the sperm sexing process this can result in damage to the plasma membrane. P2 and P3 treatment on day 7 and P2 can last until day 11 showing progressive motility ≥ 40% so that the semen can still be used for AI purposes, according to SNI standards that semen used for AI has at least progressive motility ≥ 40% (Yekti et al, 2018).…”

Kualitas Spermatozoa Cauda Epididimis Sapi Bali Pada Kelompok Umur Yang Berbeda

“…Upaya pemanfaatan spermatozoa yang berasal dari cauda epididimis saat ini telah dilakukan pada beberapa penelitian sebelumnya karena spermatozoanya memiliki potensi yang sangat baik untuk dipertahankan, seperti sapi Simmental memiliki viabilitas 78,83±6,83%, motilitas 72,25±9,11%, abnormalitas 11,00±1,26% (Satria et al, 2018), kerbau Rawa memiliki viabilitas ≥50%, motilitas ≥30% (Riyadhi et al, 2018) dan kambing Peranakan Ettawa (PE) memiliki motilitas 51,00±6,52%, viabilitas 72,00±0,84% (Rizal et al, 2016). Hasil yang diperoleh ini memberikan gambaran bahwa spermatozoa dari cauda epididimis masih memiliki kemampuan yang baik.…”