Day 6 pgs fresh embryo transfer improves pregnancy outcome and reduce miscarriage rate

Abstract

Only good and fair embryos based on SART criteria were considered suitable for biopsy. A total of 1,996 embryos were biopsied.MATERIALS AND METHODS: Trophectoderm biopsy was performed on day 5 or 6. All embryos were then vitrified, and ploidy assessed by a third party testing laboratory using array comparative genomic hybridization (aCGH). A total of 28 embryos resulted as no read (1.4%) and were not included in this analysis. Statistical comparisons were made by Chi-Square analysis.RESULTS: The average age of autologous patients was 36.8. An average of four embryos were biopsied per cycle. Overall among autologous patients, 999 embryos were found to be euploid (54%), and 847 aneuploid (46%). When isolating embryos that were biopsied on D5, 524 (59%) were euploid and 371 (41%) aneuploid. In contrast, D6 embryos yielded significantly fewer euploid results, with 475 (50%) euploid embryos and 476 (50%) aneuploid (p<0.001). This trend held true across all age groups, although did not reach significance in all comparisons. D5 embryos from donor oocyte recipients had a euploid rate of 82%, compared with D6 embryos at 70% (p>0.05). D5 embryos from patients under 35 were 73% euploid, while D6 embryos were 66% (p>0.05). For patients aged 35-37, D5 embryos were 63% euploid, with their D6 counterparts 58% (p>0.05). D5 embryos from patients aged 38-40 were 52% euploid, and D6 were 39% (p<0.05). Patients over 40 showed a D5 euploid rate of 28%, and D6 embryos 23% (p>0.05).CONCLUSIONS: Overall, embryos that were biopsied on D5 are significantly more likely to yield euploid results than their D6 counterparts. Further studies will be necessary to determine if this trend among age groups holds true, and to examine the effect of ploidy and day of biopsy on subsequent implantation success for frozen embryo transfers. Such data could help guide clinical decisions regarding patient recruitment for PGS as well as embryo selection for frozen embryo transfer in non-PGS cases.