Data on microbial community composition of sludge from high altitude wastewater treatment plants determined by 16S rRNA gene sequencing

Abstract

This work presented the sequences of activated sludge from two municipal wastewater treatment plants (WWTPs) located in a high altitude Plateau in Tibet, China (∼3650 m above the sea level). Sequencing data are the 16S rRNA gene amplicons of V4—V5 region that sequenced on an Illumina HiSeq PE250 platform. Data presented here include detail description and water quality parameters of the WWTPs as well as results of 16S rRNA gene sequences from their active sludges. The core microbial communities in the WWTPs were shown at the taxonomic level of phylum, class, order, family, genus and species. The sequencing data have been deposited in NCBI BioProject PRJNA477990 with the Biosample accessions SAMN09488330-SAMN09488338. The annotation of OTU table at the genus level was assessable on Zenodo ( https://zenodo.org/record/2105899#.XA0vQPZuJyw ).

Short‐Term Ingestion of Essential Amino Acid Based Nutritional Supplements or Whey Protein Improves the Physical Function of Older Adults Independently of Gut Microbiome

“…[ 65 ] In brief, V4 region was amplified using the barcoded primer set: 515FB: 5“‐49 GTGYCAGCMGCCGCGGTAA‐3” and 806RB: 5“GGACTACNVGGGTWTCTAAT‐3” described. [ 66 ] Then, the amplicons were sequenced using Illumina MiSeq sequencing platform by generating paired‐end reads with 2 × 250 kit.…”

Reforestation regulated soil bacterial community structure along vertical profiles in the Loess Plateau

“…The quality of the library was initially determined with a Qubit@2.0 Fluorometer (Thermo Scientific) and then Agilent Bioanalyzer 2,100 System (Agilent Technologies). Sequencing analysis was performed on an Illumina MIseq platform ( Chen et al, 2019 ) with a PE250 strategy. Raw reads (2 × 250 bp paired-end) were analyzed mainly through the pipeline of Quantitative Insights into Microbial Ecology (QIIME) ( Caporaso et al, 2010 ).…”

Effects of Various Carbon Nanotubes on Soil Bacterial Community Composition and Structure

“…The quality of the library was initially determined with a Qubit@2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 System (Agilent Technologies), and then sequencing analysis was performed on an Illumina PE250 platform. 20 Raw reads (2 × 250 bp paired-end) obtained from MiSeq high-throughput sequencing were analyzed with the pipeline of Quantitative Insights into Microbial Ecology (QIIME). 21 Chimeras were filtered out using the QIIME's default program ChimeraSlayer, 22 and reads were clustered into operational taxonomic units (OTUs) using UPARSE, 23 with a threshold of 97% identity.…”

“…The PCR products were purified, and then the sequencing libraries were established using a TruSeqDNA PCR-Free Sample Preparation Kit (Illumina) following the manufacturer’s protocols. The quality of the library was initially determined with a Qubit@2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 System (Agilent Technologies), and then sequencing analysis was performed on an Illumina PE250 platform . Raw reads (2 × 250 bp paired-end) obtained from MiSeq high-throughput sequencing were analyzed with the pipeline of Quantitative Insights into Microbial Ecology (QIIME) .…”