Das antibiotische Verhalten einiger holzzersetzender Basidiomyceten zueinander und zu Bakterien

Oppermann, Adolf

doi:10.1007/bf00408971

Cited by 29 publications

(2 citation statements)

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“…In the earliest observations, Armillaria mellea culture fluids were shown to inhibit the growth of Pseudomonas pyocanea, indicating an antibiotic activity. [1] Other melleolide derivatives were found to inhibit Gram-positive bacteria, fungi and cancer cell lines, and yet others showed no apparent activity. Interestingly, A. mellea tablets are used in Chinese medicine for a range of neurological indications such as neurasthenia, insomnia, vertigo, and headaches.…”

Section: Introductionmentioning

confidence: 99%

“…The melleolide family of sesquiterpene aryl esters is an intriguing class of fungal secondary metabolites with diverse biological activities. In the earliest observations, Armillaria mellea culture fluids were shown to inhibit the growth of Pseudomonas pyocanea , indicating an antibiotic activity [1] . Other melleolide derivatives were found to inhibit Gram‐positive bacteria, fungi and cancer cell lines, and yet others showed no apparent activity.…”

Section: Introductionmentioning

confidence: 99%

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CYPArm2 is a CYP450 Monooxygenase with Protoilludene 13‐Hydroxylase Activity Involved in the Biosynthesis of Armillyl Orsellinate‐Type Sesquiterpenoids

et al. 2023

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The melleolides are a family of structurally and functionally diverse sesquiterpenoids with potential applications as fungicides, antimicrobials, and cancer therapeutics. The initial and terminal steps of the biosynthesis pathway in Armillaria spp. have been characterized, but the intermediate steps are unclear. Biosynthetic gene clusters in A. mellea and A. gallica were shown to encode a terpene cyclase, a polyketide synthase, and four CYP450 monooxygenases. We have characterized CYPArm3, which is responsible for the hydroxylation of Δ‐6‐protoilludene, but the functions of the other CYP450s remain to be determined. Here we describe CYPArm2, which accepts Δ‐6‐protoilludene and 8α‐hydroxy‐6‐protoilludene as substrates. To investigate the products in more detail, we generated recombinant Saccharomyces cerevisiae strains overexpressing CYPArm2 in combination with the previously characterized protoilludene synthase and 8α‐hydroxylase. Using this total biosynthesis approach, sufficient quantities of product were obtained for NMR spectroscopy. This allowed the identification of 8α,13‐dihydroxy‐protoilludene, confirming that CYPArm2 is a protoilludene 13‐hydroxylase.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%