Dark exposure of petunia cuttings strongly improves adventitious root formation and enhances carbohydrate availability during rooting in the light

Klopotek, Yvonne; Haensch, Klaus-Thomas; Hause, Bettina; Hajirezaei, Mohammad‐Reza; Druege, Uwe

doi:10.1016/j.jplph.2009.11.008

Cited by 58 publications

(73 citation statements)

References 21 publications

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“…Auxin (Jasik and Klerk, 1997;Husen and Pal, 2007), nitric oxide, and hydrogen peroxide (Liao et al, 2010) treatments increase total soluble sugar levels. Furthermore, it has been demonstrated that increased rooting of cuttings kept in low light can be linked to an increase in soluble sugar (Druege et al, 2004;Druege and Kadner, 2008;Husen, 2008;Klopotek et al, 2010).…”

Section: Adventitious Root Initiationmentioning

confidence: 99%

The Physiology of Adventitious Roots

2015

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Adventitious roots are plant roots that form from any nonroot tissue and are produced both during normal development (crown roots on cereals and nodal roots on strawberry [Fragaria spp.]) and in response to stress conditions, such as flooding, nutrient deprivation, and wounding. They are important economically (for cuttings and food production), ecologically (environmental stress response), and for human existence (food production). To improve sustainable food production under environmentally extreme conditions, it is important to understand the adventitious root development of crops both in normal and stressed conditions. Therefore, understanding the regulation and physiology of adventitious root formation is critical for breeding programs. Recent work shows that different adventitious root types are regulated differently, and here, we propose clear definitions of these classes. We use three case studies to summarize the physiology of adventitious root development in response to flooding (case study 1), nutrient deficiency (case study 2), and wounding (case study 3).

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Section: Adventitious Root Initiationmentioning

confidence: 99%

The Physiology of Adventitious Roots

2015

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“…In the hypocotyls of etiolated seedlings (12 and 9 days dark) root initials even occurred at the time of explant excision, whereas no initials had been formed in hypocotyls of nontreated seedlings. Correspondingly, Klopotek et al (2010) reported that in petunia cuttings root meristem formation had already started during the dark treatment and was enhanced during the rooting period.…”

Section: Etiolationmentioning

confidence: 87%

“…Both pre-treatments also strongly lowered the endogenous Light changes also rootability of cuttings when the donor plant has been treated. Keeping donor plants for some period (weeks) in the dark, a pre-treatment usually referred to as etiolation, often improves the rootability of cuttings (Hammerschlag et al 1987;Klopotek et al 2010;Koukourikou-Petridou 1998;Shi and Brewbaker 2006). Researchers have attempted to relate the stimulation by etiolation with anatomical, physiological and molecular changes (Maynard and Bassuk 1988;Haissig and Davis 1994;Hartmann et al 2011;Sorin et al 2005) but the mechanism is still not understood.…”

Section: Introductionmentioning

confidence: 99%

Etiolation and flooding of donor plants enhance the capability of Arabidopsis explants to root

Massoumi

Krens

Visser

et al. 2017

Plant Cell Tiss Organ Cult

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“…Conventional propagation using cuttings commonly relies on cuttings being collected and stored under cold conditions (0.5 to 5°C) to slow metabolism, thus allowing for later transport to other locations (Garrido et al 1996;Druege et al 2000;Klopotek et al 2010). Whereas cold, dark storage of conventional cuttings had a beneficial effect on subsequent rooting compared to non-cold treated cuttings (Klopotek et al 2010), S. marilandica microcuttings, whether cold stored (8, 12, or 16 wk post-subculture; 6°C, dark, 7 wk) or maintained at ambient conditions (6, >20 wk post-subculture, 23±3°C, 14-h photoperiod, ca.…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of Spigelia marilandica L.

Kitto

2015

In Vitro Cell.Dev.Biol.-Plant

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Commercial micropropagation of Spigelia marilandica (Loganiaceae) requires the development of proliferation and rooting protocols for in vitro-generated microcuttings. Seedling microshoot explants formed the most axillary shoots when cultured on half-strength Murashige and Skoog basal medium supplemented with 11 or 22 μM benzyladenine. Monthly serial subculture of shoot cultures for 6 mo had no impact on the mean total number of axillary shoots formed. Microshoot explants with shoot tips formed more axillary shoots compared to single nodes, and microshoot explants with basal nodes formed more axillary shoots compared to microshoot explants without basal nodes. Microcuttings were rooted ex vitro. Microcuttings, 1-2 versus 2-5 cm in length, rooted equally well whether placed in RediEarth, sand, or vermiculite. After potting, plants from short and long microcuttings grew equally well based on leaf number and stem height; however, plants grown in the sun had more leaves and were taller than plants under 50% shade (6 versus 3.1 cm in height, respectively). Microcuttings dipped in indolebutyric acid, 500 or 1000 ppm, had more roots with longer mean total root lengths compared to control microcuttings. Microcuttings grown under mist for 4 wk survived better than those under mist for 6 wk. Neither microcutting age (6, 8, 12, 16, or >20 wk post-subculture) nor cold storage (8, 12, or 16 wk post-subculture; 6°C for 7 wk) affected the percent of shoots with roots (100%), mean root number, or mean total root length. Field-grown in vitroderived plants (60% survival) had more stems and more leaves compared to field-grown seedlings of comparable age (90% survival). Micropropagation of S. marilandica on a commercial scale has practicality based on the ease with which microshoot explants formed axillary shoots in vitro and microcuttings rooted ex vitro.

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Dark exposure of petunia cuttings strongly improves adventitious root formation and enhances carbohydrate availability during rooting in the light

Cited by 58 publications

References 21 publications

The Physiology of Adventitious Roots

The Physiology of Adventitious Roots

Etiolation and flooding of donor plants enhance the capability of Arabidopsis explants to root

Micropropagation of Spigelia marilandica L.

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