Dark conditions enhance aluminum tolerance in several rice cultivars via multiple modulations of membrane sterols

Wagatsuma, Tadao; Maejima, Eriko; Toyomasu, Tomonobu; Kuroda, Masaharu; Muranaka, Toshiya; Ohyama, Kiyoshi; Ishikawa, Akifumi; Usui, Masami; Khan, Md. Shahadat Hossain; Maruyama, Hayato; Tawaraya, Keitaro; Kobayashi, Yuriko; Koyama, Hiroyuki

doi:10.1093/jxb/erx414

Cited by 5 publications

(2 citation statements)

References 44 publications

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“…To evaluate the role of GmMYB183 in Al tolerance, six homozygous T3 transgenic Arabidopsis lines were carefully chosen for subsequent phenotypic and physiological analysis ( Figure S5 ). Relative root growth is one of the most important indices for evaluating Al tolerance in plants [ 52 ]. Exposure to Al stress exerted a concentration-dependent inhibition of Arabidopsis root growth ( Figure 4 a).…”

Section: Resultsmentioning

confidence: 99%

GmMYB183, a R2R3-MYB Transcription Factor in Tamba Black Soybean (Glycine max. cv. Tamba), Conferred Aluminum Tolerance in Arabidopsis and Soybean

Wei,

Han,

2024

Biomolecules

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Aluminum (Al) toxicity is one of the environmental stress factors that affects crop growth, development, and productivity. MYB transcription factors play crucial roles in responding to biotic or abiotic stresses. However, the roles of MYB transcription factors in Al tolerance have not been clearly elucidated. Here, we found that GmMYB183, a gene encoding a R2R3 MYB transcription factor, is involved in Al tolerance. Subcellular localization studies revealed that GmMYB183 protein is located in the nucleus, cytoplasm and cell membrane. Overexpression of GmMYB183 in Arabidopsis and soybean hairy roots enhanced plant tolerance towards Al stress compared to the wild type, with higher citrate secretion and less Al accumulation. Furthermore, we showed that GmMYB183 binds the GmMATE75 gene promoter encoding for a plasma-membrane-localized citrate transporter. Through a dual-luciferase reporter system and yeast one hybrid, the GmMYB183 protein was shown to directly activate the transcription of GmMATE75. Furthermore, the expression of GmMATE75 may depend on phosphorylation of Ser36 residues in GmMYB183 and two MYB sites in P3 segment of the GmMATE75 promoter. In conclusion, GmMYB183 conferred Al tolerance by promoting the secretion of citrate, which provides a scientific basis for further elucidating the mechanism of plant Al resistance.

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Section: Resultsmentioning

confidence: 99%

GmMYB183, a R2R3-MYB Transcription Factor in Tamba Black Soybean (Glycine max. cv. Tamba), Conferred Aluminum Tolerance in Arabidopsis and Soybean

Wei,

Han,

2024

Biomolecules

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“…One Al-tolerant Japonica variety, named Nipponbare, was used to identify Al-tolerant mechanisms in rice [ 23 ]. Wagatsuma et al investigated the Al-tolerance mechanism with five temperate Japonica varieties, including two Al-sensitive and three Al-tolerant varieties, as well as one Al-sensitive Indica variety [ 24 ]. However, the Al-tolerant genotypes used in the previous studies were Japonica or mutants originating from Japonica.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis for Indica and Japonica Rice Varieties under Aluminum Toxicity

Zhang

Ding

Zhong

et al. 2019

IJMS

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Aluminum (Al) at high concentrations inhibits root growth, damage root systems, and causes significant reductions in rice yields. Indica and Japonica rice have been cultivated in distinctly different ecological environments with different soil acidity levels; thus, they might have different mechanisms of Al-tolerance. In the present study, transcriptomic analysis in the root apex for Al-tolerance in the seedling stage was carried out within Al-tolerant and -sensitive varieties belonging to different subpopulations (i.e., Indica, Japonica, and mixed). We found that there were significant differences between the gene expression patterns of Indica Al-tolerant and Japonica Al-tolerant varieties, while the gene expression patterns of the Al-tolerant varieties in the mixed subgroup, which was inclined to Japonica, were similar to the Al-tolerant varieties in Japonica. Moreover, after further GO (gene ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses of the transcriptomic data, we found that eight pathways, i.e., “Terpenoid backbone biosynthesis”, “Ribosome”, “Amino sugar and nucleotide sugar metabolism”, “Plant hormone signal transduction”, “TCA cycle”, “Synthesis and degradation of ketone bodies”, and “Butanoate metabolism” were found uniquely for Indica Al-tolerant varieties, while only one pathway (i.e., “Sulfur metabolism”) was found uniquely for Japonica Al-tolerant varieties. For Al-sensitive varieties, one identical pathway was found, both in Indica and Japonica. Three pathways were found uniquely in “Starch and sucrose metabolism”, “Metabolic pathway”, and “Amino sugar and nucleotide sugar metabolism”.

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Plant responses to soil-borne ion toxicities

Rengel¹

2023

Marschner's Mineral Nutrition of Plants

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Dark conditions enhance aluminum tolerance in several rice cultivars via multiple modulations of membrane sterols

Abstract: Aluminum tolerance of aluminum-sensitive rice was enhanced under darkness by multiple changes in membrane sterols: decreased stigmasterol, increased precursor partitioning for sterols biosynthesis, and increased expression of HMG genes.

Cited by 5 publications

References 44 publications

GmMYB183, a R2R3-MYB Transcription Factor in Tamba Black Soybean (Glycine max. cv. Tamba), Conferred Aluminum Tolerance in Arabidopsis and Soybean

GmMYB183, a R2R3-MYB Transcription Factor in Tamba Black Soybean (Glycine max. cv. Tamba), Conferred Aluminum Tolerance in Arabidopsis and Soybean

Transcriptomic Analysis for Indica and Japonica Rice Varieties under Aluminum Toxicity

Plant responses to soil-borne ion toxicities

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