Dark‑colored maple syrup treatment induces S‑phase cell cycle arrest via reduced proliferating cell nuclear antigen expression in colorectal cancer cells

Yamamoto, Tetsushi; Nishita, Tomoyo; Taga, Atsushi

doi:10.3892/ol.2019.9928

Cited by 5 publications

(5 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…in the present study, rom-1 levels were lower in the retinas of STZ rats than normal rats. as the results of semi-quantitative analysis based on spectral counts highly correlate with the results of quantification by Western blot in previous studies (12,45,46), the expression of Table V. Proteins categorized as 'protein binding' in Gene ontology analysis.…”

Section: Discussionsupporting

confidence: 68%

Retinal proteomic evaluation of rats following streptozotocin‑injection using shotgun proteomics

et al. 2019

View full text Add to dashboard Cite

it is important to elucidate how retinal stimulation leads to retinal protection and dysfunction. The current study aimed to identify factors that are up-and downregulated in the retinas of streptozotocin (STZ)-induced diabetic rats with acute retinal dysfunction. retinal function was measured and changes in protein expressions were determined using electroretinograms (erGs) and liquid chromatography/mass spectroscopy-based shotgun proteomics, respectively. The results revealed that the plasma glucose levels of STZ rats were markedly higher when compared with normal rats. Furthermore, levels of a-waves, b-waves and oscillatory potential amplitudes on erGs in STZ rats were decreased compared with healthy animals. With use of shotgun proteomics, 391 proteins were identified in the retinas of normal rats and 541 proteins were found in the retinas of STZ rats. Of the 560 proteins identified in rat retinas, 372 (66.4%) were present in both normal and STZ rats. of these, 19 (3.39%) were unique to normal rats and 169 (30.1%) were unique to STZ rats. Gene ontology analysis was performed on the candidate proteins that were differentially regulated in the retinas of STZ rats and focused on those classified as 'protein binding', which serve important roles in retinal neurodegeneration. The results revealed an excessive expression of retinol-binding protein 1 (rBP1) and a negative expression of rod outer segment membrane protein 1 (rom-1) in the retinas of STZ rats. Therefore, retinal function may be decreased with STZ-induced injury, and expressions of rom-1 and rBP1 may be altered in the retinas of STZ rats.

show abstract

Section: Discussionsupporting

confidence: 68%

Retinal proteomic evaluation of rats following streptozotocin‑injection using shotgun proteomics

et al. 2019

View full text Add to dashboard Cite

show abstract

“…This suggested that autophagy was induced to protect cancer cells against MSpf treatment. Since inhibition of the STAT3 signaling pathway is associated with not only induction of autophagy but also induction of apoptosis and cell cycle arrest ( 55 – 57 ), in addition to our previous study showing that maple syrup treatment induces S-phase cell cycle arrest ( 14 ), AGEs in MSpf may have induced cell cycle arrest.…”

Section: Discussionmentioning

confidence: 84%

“…Maple syrup is a better alternative to refined sugar in the human diet owing to its low glycemic and insulinemic response properties (11,12). In addition, it was previously reported that maple syrup has an anti-tumor effect against gastrointestinal cancer cells, including CRC (13)(14)(15). Therefore, maple syrup may be a useful resource to investigate phytochemicals for CRC treatment.…”

Section: Introductionmentioning

confidence: 99%

Protein components of maple syrup as a potential resource for the development of novel anti‑colorectal cancer drugs

Yamamoto,

Shiburo,

Moriyama

et al. 2023

Oncol Rep

Self Cite

View full text Add to dashboard Cite

Maple syrup is a natural sweetener consumed worldwide. Active ingredients of maple syrup possess antitumor effects; however, these ingredients are phenolic compounds. The present study aimed to investigate components other than phenolic compounds that may have antitumor effects against colorectal cancer (CRC). Cell proliferation assays demonstrated that treatment with the more than 10,000 molecular weight fraction significantly inhibited viability in DLD-1 cells. Therefore, we hypothesized that the protein components of maple syrup may be the active ingredients in maple syrup. We obtained protein components from maple syrup by ammonium sulfate precipitation, and treatment with the protein fraction of maple syrup (MSpf) was found to exhibit a potential antitumor effect. MSpf-treated DLD-1 colon adenocarcinoma cells exhibited significantly decreased proliferation, migration and invasion. In addition, upregulation of LC3A and E-cadherin and downregulation of MMP-9 expression levels were observed following MSpf treatment. Investigation of the components of MSpf suggested that it was primarily formed of advanced glycation end products (AGEs). Therefore, whether AGEs in MSpf affected the STAT3 pathway through the binding to its receptor, receptor of AGE (RAGE), was assessed. MSpf treatment was associated with decreased RAGE expression and STAT3 phosphorylation. Finally, to determine whether autophagy contributed to the inhibitory effect of cell proliferation following MSpf treatment, the effect of MSpf treatment on autophagy induction following bafilomycin A1 treatment, a specific autophagy inhibitor, was assessed. The inhibitory effect of MSpf treatment on cell proliferation was enhanced through the inhibition of autophagy by bafilomycin A1 treatment. These results suggested that AGEs in MSpf suppressed cell proliferation and epithelial-mesenchymal transition through inhibition of the STAT3 signaling pathway through decreased RAGE expression. Therefore, AGEs in MSpf may be potential compounds for the development of antitumor drugs for the treatment of CRC with fewer adverse effects compared with existing antitumor drugs.

show abstract

“…As the season progresses, maple syrup’s color darkens, and it is classified into four grades: golden, amber, dark, and very dark [ 1 ]. Maple syrup has various biological effects, and it has also been reported that different grades of maple syrup could have different biological effects [ 2 , 3 , 4 , 5 , 6 ]. Although maple syrup has a low glycemic index (GI), it is mostly sucrose with a small amount of glucose and fructose [ 5 , 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Separation of Fructosyl Oligosaccharides in Maple Syrup by Using Charged Aerosol Detection

Sato

Yamamoto

Mitamura

et al. 2021

Foods

Self Cite

View full text Add to dashboard Cite

Fructosyl oligosaccharides, including fructo-oligosaccharide (FOS), are gaining popularity as functional oligosaccharides and have been found in various natural products. Our previous study suggested that maple syrup contains an unidentified fructosyl oligosaccharide. Because these saccharides cannot be detected with high sensitivity using derivatization methods, they must be detected directly. As a result, an analytical method based on charged aerosol detection (CAD) that can detect saccharides directly was optimized in order to avoid relying on these structures and physical properties to clarify the profile of fructosyl oligosaccharides in maple syrup. This analytical method is simple and can analyze up to hepta-saccharides in 30 min. This analytical method was also reliable and reproducible with high validation values. It was used to determine the content of saccharides in maple syrup, which revealed that it contained not only fructose, glucose, and sucrose but also FOS such as 1-kestose and nystose. Furthermore, we discovered a fructosyl oligosaccharide called neokestose in maple syrup, which has only been found in a few natural foods. These findings help to shed light on the saccharides profile of maple syrup.

show abstract

Dark‑colored maple syrup treatment induces S‑phase cell cycle arrest via reduced proliferating cell nuclear antigen expression in colorectal cancer cells

Cited by 5 publications

References 59 publications

Retinal proteomic evaluation of rats following streptozotocin‑injection using shotgun proteomics

Retinal proteomic evaluation of rats following streptozotocin‑injection using shotgun proteomics

Protein components of maple syrup as a potential resource for the development of novel anti‑colorectal cancer drugs

Separation of Fructosyl Oligosaccharides in Maple Syrup by Using Charged Aerosol Detection

Contact Info

Product

Resources

About