Dandelion Extracts Protect Human Skin Fibroblasts from UVB Damage and Cellular Senescence

Yang, Yanming; Li, Shuangshuang

doi:10.1155/2015/619560

Cited by 36 publications

(38 citation statements)

References 23 publications

(56 reference statements)

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“…Various herbal extracts have shown beneficial properties as indicated in some studies. [2125] We examined A. vera , henna, A. capillus-veneris and C. molmol extracts and their mixture for treatment of normal mouse skin fibroblast line. It was demonstrated that methanolic extract of A. capillus-veneris and C. molmol and ethanolic extract of hennasignificantly improved the expression of Tgfβ1 and Vegf-A genes at 48 h after treatment of fibroblast cells.…”

Section: Discussionmentioning

confidence: 99%

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Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells

et al. 2017

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Background:Among the most important factors in wound healing pathways are transforming growth factor beta1 and vascular endothelial growth factor. Fibroblasts are the main cell in all phases wound closure. In this study, the extracts of plant materials such as Adiantum capillus-veneris, Commiphora molmol, Aloe vera, and henna and one mixture of them were used to treatment of normal mouse skin fibroblasts.Methods:Cytotoxic effects of each extract and their mixture were assessed on mouse skin fibroblasts cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. We performed migration assays to assess migration properties of mouse skin fibroblasts cells in response to the extracts. Changes in the gene expression of the Tgfβ1 and Vegf-A genes were monitored by real-time polymerase chain reaction.Results:A. capillus-veneris, C. molmol and henna extract improved the expression of Tgfβ1 gene. All used extracts upregulated the expression of Vegf-A gene and promoted the migration of mouse fibroblast cells in vitro.Conclusions:The present study demonstrated that the mentioned herbal extracts might be effective in wound healing, through the improvement in the migration of fibroblast cells and regulating the gene expression of Tgfβ1 and Vegf-A genes in fibroblast cells treated with extracts.

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Section: Discussionmentioning

confidence: 99%

“…Subsequently, intracellular formazan can be solubilized and distinguished by spectrophotometric means. [2526]…”

Section: Methodsmentioning

confidence: 99%

Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells

et al. 2017

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“…Dermal fibroblasts, which mainly comprise the dermis layer of the skin, are established to contribute to the generation of connective tissues4243. During skin aging, MMPs, which are generated from dermal fibroblasts, can degrade all components in the ECM, including collagen, elastin, laminin, and proteoglycans44.…”

Section: Discussionmentioning

confidence: 99%

Pretreatment of Ferulic Acid Protects Human Dermal Fibroblasts against Ultraviolet A Irradiation

et al. 2016

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BackgroundApproximately 90%~99% of ultraviolet A (UVA) ray reaches the Earth's surface. The deeply penetrating UVA rays induce the formation of reactive oxygen species (ROS), which results in oxidative stress such as photoproducts, senescence, and cell death. Thus, UVA is considered a primary factor that promotes skin aging.ObjectiveResearchers investigated whether pretreatment with ferulic acid protects human dermal fibroblasts (HDFs) against UVA-induced cell damages.MethodsHDF proliferation was analyzed using the water-soluble tetrazolium salt assay. Cell cycle distribution and intracellular ROS levels were assessed by flow cytometric analysis. Senescence was evaluated using a senescence-associated β-galactosidase assay, while Gadd45α promoter activity was analyzed through a luciferase assay. The expression levels of superoxide dismutase 1 (SOD1), catalase (CAT), xeroderma pigmentosum complementation group A and C, matrix metalloproteinase 1 and 3, as well as p21 and p16 were measured using quantitative real-time polymerase chain reaction.ResultsInhibition of proliferation and cell cycle arrest were detected in cells that were irradiated with UVA only. Pretreatment with ferulic acid significantly increased the proliferation and cell cycle progression in HDFs. Moreover, ferulic acid pretreatment produced antioxidant effects such as reduced DCF intensity, and affected SOD1 and CAT mRNA expression. These effects were also demonstrated in the analysis of cell senescence, promoter activity, expression of senescent markers, and DNA repair.ConclusionThese results demonstrate that ferulic acid exerts protective effects on UVA-induced cell damages via anti-oxidant and stress-inducible cellular mechanisms in HDFs.

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“…Водные экстракты листьев, цветов и корней одуванчика (Taraxacum officinale) тормозили стресс-индуцированное старение фибробластов кожи человека, вызванного перекисью водорода, на 62%, 73% и 40%, соответственно. При этом защитное действие экстракта одуванчика не зависело от того, был ли он добавлен до или после Н 2 О 2 [24]. Используемый в китайской медицине водный экстракт шести растений (Kangen-karyu) защищал фибробласты от стресс-индуцированного старения, как в условиях преинкубации, так и при добавлении после Н 2 О 2 .…”

Section: результаты и обсуждениеunclassified

The Sturgeon Ovarian Liquid and the Perch Roe Extract are Able to Enhance the Recovery of the Fibroblasts after their Stress-induced Premature Senescence

Mikhailova

Belyaeva

Kozlova

et al. 2018

BMCRM

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Ovarian liquid and fish roe are valuable sources of biologically active compounds. In order to study mechanisms of aging and also to search for biologically active compounds capable of inhibiting aging, we have modelled premature senescence in human embryonic fibroblasts by incubating of cells with Н2О2. Cell senescence was assessed by staining for β-galactosidase (SA-β-Gal) at pH 6.0; cell proliferation was further evaluated by the optical method. The dried ovarian liquid of the Siberian sturgeon and the extract of the perch roe were able to enhance recovery of the cells after induction of their premature senescence caused by oxidative stress. In contrast to the extract from perch roe and the extracts from fish muscle, dried ovarian fluid gave such an effect without the growth of proliferation.

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Dandelion Extracts Protect Human Skin Fibroblasts from UVB Damage and Cellular Senescence

Cited by 36 publications

References 23 publications

Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells

Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells

Pretreatment of Ferulic Acid Protects Human Dermal Fibroblasts against Ultraviolet A Irradiation

The Sturgeon Ovarian Liquid and the Perch Roe Extract are Able to Enhance the Recovery of the Fibroblasts after their Stress-induced Premature Senescence

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