Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells

Negahdari, Samira; Galehdari, Hamid; Kesmati, Mahnaz; Rezaie, Annahita; Shariati, Gholamreza

doi:10.4103/ijpvm.ijpvm_338_16

Cited by 44 publications

(26 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These relations demonstrate tissue repair-associated fibroblast migration and proliferation, and that ECM remodeling-related collagen synthesis in fibroblasts plays important roles during skin wound healing and regeneration [5,16]. Although many strategies have been devised to promote skin wound healing [17], they remain unsatisfactory, and thus control of skin cell proliferation, migration, and collagen synthesis appears to be a promising strategy for promoting skin wound healing and regeneration, Many plants and plant extracts have been used traditionally to treat diverse disorders [18], and the wound healing effects of many plant extracts have been investigated due to their minimal side effects and low manufacturing costs [1,19]. Furthermore, the development of herbal therapeutic materials that accelerate wound healing has recently drawn research attention.…”

Section: Introductionmentioning

confidence: 99%

Potential Beneficial Effects of Digitaria ciliaris Flower Absolute on the Wound Healing-Linked Activities of Fibroblasts and Keratinocytes

et al. 2020

View full text Add to dashboard Cite

Digitaria ciliaris is widely reported to be a problematic weed in agricultural areas and is mainly used as an indicator plant for the development of herbicides. However, its bioactivities on skin regeneration and wound healing have not been investigated. In the present study, we investigated the effects of D. ciliaris flower absolute on skin wound healing and skin regeneration-related events, that is, proliferation, migration, and collagen biosynthesis, in human fibroblasts and keratinocytes. For this study, we extracted absolute from the D. ciliaris flower by solvent extraction and identified the composition of D. ciliaris flower absolute using GC/MS analysis. We also tested the effect of D. ciliaris flower absolute in CCD986sk fibroblasts and/or HaCaT keratinocytes using the WST assay and 5-bromo-2′-deoxyuridine incorporation assay, Boyden chamber assay, ELISA, sprouting assay, and immunoblotting. GC/MS analysis of D. ciliaris flower absolute revealed that it contained 15 compounds. The absolute increased the proliferations of keratinocytes and fibroblasts and the migration of fibroblasts but did not affect cell viabilities. In addition, it enhanced the syntheses of type I and IV collagen in fibroblasts, but not in keratinocytes. The sprouting assay showed increased sprout outgrowth of fibroblasts. In addition, D. ciliaris flower absolute induced the phosphorylation of extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase in fibroblasts. These results indicate that D. ciliaris flower absolute may promote skin wound healing/regeneration by inducing the proliferation, migration, and collagen synthesis of fibroblasts, as well as the proliferation of keratinocytes. Therefore, D. ciliaris flower absolute may be a potential natural source for cosmetic or pharmaceutical agents that promote skin wound healing/regeneration.

show abstract

Section: Introductionmentioning

confidence: 99%

Potential Beneficial Effects of Digitaria ciliaris Flower Absolute on the Wound Healing-Linked Activities of Fibroblasts and Keratinocytes

et al. 2020

View full text Add to dashboard Cite

show abstract

“…These steps can be characterized into four highly programmed phases such as hemostasis, inflammation, proliferation and remodeling [2,6,[12][13][14][15][16]. All phases of healing must occur in suitable sequence for successful healing as a natural phenomenon [6,12,13] but sometimes complex multifactorial process of wound healing can be delayed or failed by unwanted effects such as presence of free radicals [12,17] or microbial infections [5,12,16,17].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Wound Healing Properties of Luteolin Ointments on Excision and Incision Wound Models in Diabetic and Non-Diabetic Rats

Özay¹,

Güzel²,

Erdoğdu³

et al. 2018

Rec.Nat.Prod.

View full text Add to dashboard Cite

Luteolin, which is structurally classified under the flavonoids, is naturally distributed abundant among medicinal plants, vegetables and fruits. The wound healing effects of Luteolin ointments were evaluated for incision and excision wound models on non-diabetic and Streptozotocin induced diabetic rats by using macroscopical, biomechanical, biochemical and histopathological methods. Two different concentrations (0.5 % and 1 % (w/w)) of the Luteolin ointment were prepared with the mixture of glycol stearate:propylene glycol:liquid paraffin (3:6:1) and 0.5 g of the ointments were applied topically on wounds once daily for 7 and 14 days. During the experiments, wounds were visually observed, photographically documented and wound areas were measured. After 7 and 14 days treatment, animals were sacrificed under anesthesia and hydroxyproline measurement and biomechanical analysis were performed. Histopathology of the wound area was evaluated considering features of re-epithelialization, thickness of the granulation tissue, angiogenesis, presence of inflammation, number of mast cells. Outcomes of this study revealed that Luteolin ointments improved wound healing process of skin tissue both in non-diabetic and diabetic wounds. The best wound healing activity was observed in incision and excision wounds (97.6 %, 96.1 %, respectively) treated with 0.5 % (w/w) Luteolin ointment on day 14 according to macroscopic results.

show abstract

“…In this study, herbal extracts were found to induce significant proliferation and viability of human dermal fibroblasts at concentrations ranging from 5 to 25 µL/mL. mouse fibroblast cells in vitro (15).…”

Section: Discussionmentioning

confidence: 88%

Discovering Cellular Response to Medicinal Herbs, Iranian Traditional Medicine and Modern Cosmeceutical Approaches

Salehinia

Ashtiani

Rastegar³

et al. 2016

J Skin Stem Cell

View full text Add to dashboard Cite

Background: Fibroblasts are the most prominent cells of the skin. Consequently, skin aging depends predominantly on these cells. Applying herbs in anti-aging, antioxidant, anti-pollutant, anti-bacterial, and anti-inflammatory formulas are now growing. On the basis of the fact that Iranian traditional medicine has utilized herbs for several centuries in order to improve various kinds of health issues, Rosemarinus officinalis leaf extract and Altheae officinalis root extract have been selected for evaluating their effects on viability and proliferation on HDF in comparison to modern cosmeceutical approaches including growth factor application as anti-aging ingredients. This survey has been performed for assessing the effect of herbs on proliferation, viability, and differentiation of cells. Methods: Human dermal fibroblasts (HDF) were isolated from the human foreskin. The methods used for viability measurement of fibroblasts were Tripan blue and Bromodeoxyuridine (Brdu) incorporation assays, which were used to study the cell proliferation effect of herbal extracts in HDF. Results: HDF were isolated by tissue culture. Herbal extracts and bFGF were found to induce significant proliferation and viability of HDFs at concentrations ranging from 10 to 20 µL/mL and 15 to 25 µL/mL and were reduced in presence of 1 and 5 µM of LBSO as the positive control to assess the oxidant potential of this substance. Conclusions: These results suggest that herbal extract may have inhibitory effects on senescence of dermal fibroblasts and suggesting that herbal extracts may be a good candidate to utilize in anti-aging, wound, and burn healing formulations and products.

show abstract

Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells

Cited by 44 publications

References 47 publications

Potential Beneficial Effects of Digitaria ciliaris Flower Absolute on the Wound Healing-Linked Activities of Fibroblasts and Keratinocytes

Potential Beneficial Effects of Digitaria ciliaris Flower Absolute on the Wound Healing-Linked Activities of Fibroblasts and Keratinocytes

Evaluation of the Wound Healing Properties of Luteolin Ointments on Excision and Incision Wound Models in Diabetic and Non-Diabetic Rats

Discovering Cellular Response to Medicinal Herbs, Iranian Traditional Medicine and Modern Cosmeceutical Approaches

Contact Info

Product

Resources

About