Damage to Cultivated Japanese Pearl Oysters by Oxidative Stress That Was Related to “Mass Mortality”

Uchimura, Yuushi; Yamashita, Hirofumi; Kuramoto, Makoto; Ishihara, Kohji; Sugimoto, Mineharu; Nakajima, Nobuyoshi

doi:10.1271/bbb.67.2470

Cited by 7 publications

(6 citation statements)

References 7 publications

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“…2002). Furthermore, changes in hemocyte cell state have also been correlated with field mortality, with hemocytes exhibiting diminished expression of oxidative stress response proteins and evidence of elevated DNA damage (Uchimura et al. 2003).…”

Section: Discussionmentioning

confidence: 99%

Mass mortality in Pacific oysters is associated with a specific gene expression signature

Chaney

Gracey

2011

Molecular Ecology

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Mass mortality events occur in natural and cultured communities of bivalve molluscs. The Pacific oyster, Crassostrea gigas, is a dominant species in many intertidal locations as well as an important aquacultured bivalve species, and for the last 50 years, adult oysters have suffered frequent and extreme mass mortality events during summer months. To investigate the molecular changes that precede these mortality events, we employed a novel nonlethal sampling approach to collect haemolymph samples from individual oysters during the period that preceded a mortality event. Microarray-based gene expression screening of the collected haemolymph was used to identify a mortality gene expression signature that distinguished oysters that survived the mortality event from those individuals that died during the event. The signature was cross-validated by comparing two separate episodes of mortality. Here, we report that near-mortality oysters can be distinguished from longer-lived oysters by the elevated expression of genes associated with cell death, lysosomal proteolysis, and cellular assembly and organization. These results show the potential utility of nonlethal sampling approaches for investigating the environmental causes of mortality in natural populations in the field, and for predicting when such events could occur and which individuals will be affected.

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Section: Discussionmentioning

confidence: 99%

Mass mortality in Pacific oysters is associated with a specific gene expression signature

Chaney

Gracey

2011

Molecular Ecology

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“…Cell-free haemolymph immunoblotting at 30 C showed a strong increase in Cu/Zn-SOD and Mn-SOD expression and a clear decrease in EC-SOD like-protein expression. Uchimura et al [66] in a study about massive mortality in Japanese pearl oysters found that in the cell extract of low mortality groups, SOD activity (97.7 U/ml) was higher (38.8 U/ml) than in high mortality groups (28.8%). At the same time, DNA damage and oxidative stress products were lower in low mortality groups.…”

Section: Sod Activity and Expressionmentioning

confidence: 95%

“…At the same time, DNA damage and oxidative stress products were lower in low mortality groups. Therefore, oysters presenting high SOD activity were better protected against oxidative stress [66]. DNA alterations and malondialdehyde (MDA) presence showed that negative effects of oxyradicals, which determined the destruction of circulatory organs and the cytolysis of haemocytes, were found only in high mortality oysters.…”

Section: Sod Activity and Expressionmentioning

confidence: 99%

Effects of high temperatures on functional responses of haemocytes in the clam Chamelea gallina

Monari

Matozzo

Foschi

et al. 2007

Fish & Shellfish Immunology

162

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“…Oxidative stress, defined as the imbalance between oxidant and antioxidants in favor of the oxidants, potentially leading to damage, has been associated with a number of diseases [21,22] . Moreover, it was found that oxidative stress is associated with abnormal changes that may lead to disturbances in some physiological processes in the brain and the liver that could render these animals susceptible to infectious diseases and ultimately to death if not treated either in human or animals [23][24][25] .…”

Section: Resultsmentioning

confidence: 99%

Elucidation of the Determinant Factors Affecting Camels’ Health in Some Regions of Saudi Arabia: A Biochemical, Histological, and Toxicological Study

Jalal¹,

Albuseeda²,

Kumosani³

et al. 2010

sci

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Recent reports announced by the Saudi Ministry of Agriculture indicate the death of more than 3,000 camels, cows and goats in different parts of the Kingdom over the past months. Laboratories examinations both inside and outside Saudi Arabia suggested that the samples contained poisonous and toxic materials to camels which may affect the physiological processes that may render animals vulnerable to pathogens such as bacteria and viruses. So, we aimed to investigate blood and plasma chemistry including analysis of oxidants and antioxidants parameters as well as heavy metal determination in blood and tissue samples from diseased animals and compare it to normal animals to elucidate the possible cause(s) of the intoxication. Although we could not obtain any samples from diseased animals, results showed that there were non significant changes in biochemical, heavy metals and blood picture in specimens from the normal animals from Jeddah and Taif areas. However, there was a significant elevation in the level of malondialdhyde (lipid peroxidation marker) in brain, liver and serum associated with a significant decrease in total antioxidant activity in specimens from Jeddah area. This imbalance between oxidants and antioxidants as a marker of abnormal changes may lead to disturbances in some physiological processes in the brain and the liver that could render these animals susceptible to infectious diseases and ultimately to death if not treated.

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Damage to Cultivated Japanese Pearl Oysters by Oxidative Stress That Was Related to “Mass Mortality”

Cited by 7 publications

References 7 publications

Mass mortality in Pacific oysters is associated with a specific gene expression signature

Mass mortality in Pacific oysters is associated with a specific gene expression signature

Effects of high temperatures on functional responses of haemocytes in the clam Chamelea gallina

Elucidation of the Determinant Factors Affecting Camels’ Health in Some Regions of Saudi Arabia: A Biochemical, Histological, and Toxicological Study

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