Daidzin inhibits growth and induces apoptosis through the JAK2/STAT3 in human cervical cancer HeLa cells

Abstract

Daidzin, 4′, 7-dihydroxyisoflavone is an isoflavonic phytoestrogen present in leguminous plants. Traditional Chinese medicine utilizes daidzin to treat various diseases such diarrhea, fever, hepatitis, cardiac problems etc. In current study we examined the anticancer activity of daidzin against human cervical cancer in vitro. HeLa, human cervical cancer cell line was purchased from ATCC and the cells were cultured with DMEM medium. The cytotoxic effect of daidzin against HeLa cell line was analyzed with MTT assay. The IC-50 value was obtained at 20 µM hence the cells were treated with 20 µM of daidzin for further analysis. ROS generation was assessed with DCFH-DA staining and the induction of apoptosis was examined with Rhoadmine-123 staining. Acridine orange and ethidium bromide staining was done to examine the apoptotic and viable cells. Further the matrigel cell adhesion assay was done to analyze the inhibitory property of daidzin against cancer cell adhesion. Apoptotic induction of daidzin was examined by estimating the levels of Caspase 8 & 9 using ELISA technique. Inflammatory and cell proliferation signaling proteins were analyzed with qPCR analysis to confirm the anticancer activity of daidzin against human cervical cancer HeLa cell line. Daidzin significantly generated ROS and altered the mitochondrial membrane permeability in HeLa cell line. The results of AO/EtBr staining prove daidzin induced apoptosis in HeLa cell line and it also inhibited the cell adhesion property of HeLa which is reported in our matrigel cell adhesion assay. It also increased the caspases 8 & 9 which are key regulators of apoptosis. Daidzin significantly decreased the expression of inflammatory gene and cell proliferating signaling molecule. To, conclude our results confirm daidzin effectively decreased inflammation and induced apoptosis in human cervical cancer HeLa cell line.

Physiological and Metabolic Changes in Tamarillo (Solanum betaceum) during Fruit Ripening

“…Rosmarinic acid (RA) is a classical type of polyphenol that exhibits great potentially beneficial pharmacological functions such as anticarcinogenic, antiallergic, anti-inflammatory, and antimicrobial [ 22 ]. Daidzin is one of the major isoflavone glycosides and is an antioxidant isoflavonoid, which shows decreased blood alcohol levels, liver protection, and decreased inflammation activity [ 23 , 24 , 25 ]. Quercetin-3-O-sophoroside is an important glucose-bound derivative of quercetin with a dopaminergic neuroprotection effect and strong antioxidant capacity [ 26 , 27 ].…”

Anticancer potential of phytochemicals from Oroxylum indicum targeting Lactate Dehydrogenase A through bioinformatic approach

“…Daidzin and Troxerutin were predicted as similar analogs for Chrysin-7-O-glucuronide. Daidzin has been reported to show suppressive effects on rat prostate carcinogenesis as well as to inhibit growth and induce apoptosis in HeLa cell lines [78] , [79] . Several in vitro investigations using various cancer cell lines demonstrated anticancer and cytotoxic properties of Troxerutin [80] , [81] , [82] .…”

Ponicidin Induces Apoptosis and Inhibits Tumor Growth in C666-1 Nasopharyngeal Cancer Cells

“…Oxidative stress in cancer cells is brought about by an increase in ROS, which limits the synthesis of antioxidants (Yao et al, 2021). SOD enables the catalysis of superoxide anions to oxygen and hydrogen peroxide conversion while providing protection against lipid peroxidation.…”