Daidzein inhibits carbohydrate digestive enzymes in vitro and alleviates postprandial hyperglycemia in diabetic mice

Park, Mi Hwa; Ju, Jae Won; Park, Min Jung; Han, Jiarui

doi:10.1016/j.ejphar.2013.04.047

Cited by 73 publications

(49 citation statements)

References 43 publications

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“…Among the many therapies, decreasing postprandial hyperglycemia is considered to be the best therapeutic approach (Park et al, 2013;Toeller, 1994). It is achieved through retarding the absorption of glucose by inhibiting activities of the carbohydrate hydrolyzing enzymes (a-amylase and a-glucosidase) in the digestive tract.…”

Section: Introductionmentioning

confidence: 99%

Effects ofGrifola frondosanon-polar bioactive components on high-fat diet fed and streptozotocin-induced hyperglycemic mice

Shen

et al. 2014

Pharmaceutical Biology

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Ng (2015) Effects of Grifolafrondosa non-polar bioactive components on high-fat diet fed and streptozotocin-induced hyperglycemic mice, Pharmaceutical Biology, 53:5, 705-709, DOI: 10.3109/13880209.2014 In vitro hypoglycemic effects of GF was evaluated enzymatically using a-amylase and a-glucosidase inhibition assays, whereas in vivo study was conducted on high-fat diet fed and streptozotocin (HFD + STZ)-induced hyperglycemic mice. GC-MS was used to determine the chemical profiles of bioactive components.Results: The non-polar fraction of GF exhibited a stronger anti-a-glucosidase activity (IC 50 : 0.0332 mg/ml) than acarbose, but its anti-a-amylase activity (IC 50 : 0.671 mg/ml) was weaker. Oral administration of GF at 600 mg/kg (GF600) significantly lowered the blood glucose, HbA1c, average blood glucose, and serum total cholesterol levels in hyperglycemic mice. Although GF was found to contain mainly oleic acid and linoleic acid, their levels in the fungus were low, suggesting that the effects of GF on HFD + STZ-induced hyperglycemic mice could be due to factors other than these fatty acids. Conclusion: These results conclude that GF possesses anti-a-glucosidase activity, and hypoglycemic effect in HFD + STZ-induced hyperglycemic mice.

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Section: Introductionmentioning

confidence: 99%

Effects ofGrifola frondosanon-polar bioactive components on high-fat diet fed and streptozotocin-induced hyperglycemic mice

Shen

et al. 2014

Pharmaceutical Biology

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“…Isoflavones such as daidzein exert their anti-inflammatory properties in various disease models [19,20] . We hypothesize that the anti-inflammatory effect of daidzein is mediated by impairing the activity of PARP, which would thereby decrease the expression of inflammatory genes.…”

Section: Introductionmentioning

confidence: 99%

Daidzein suppresses pro-inflammatory chemokine Cxcl2 transcription in TNF-α-stimulated murine lung epithelial cells via depressing PARP-1 activity

Pan

et al. 2014

Acta Pharmacol Sin

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Aim: Daidzein (4′,7-dihydroxyisoflavone) is an isoflavone exiting in many herbs that has shown anti-inflammation activity. The aim of this study was to investigate the mechanism underlying its anti-inflammatory action in murine lung epithelial cells. Methods: C57BL/6 mice were intranasally exposed to TNF-α to induce lung inflammation. The mice were injected with daidzein (400 mg/kg, ip) before TNF-α challenge, and sacrificed 12 h after TNF-α challenge, and lung tissues were collected for analyisis. In in vitro studies, murine MLE-12 epithelial cells were treated with TNF-α (20 ng/mL). The expression of pro-inflammatory chemokine Cxcl2 mRNA and NF-κB transcriptional activity were examined using real-time PCR and a dual reporter assay. Protein poly-adenosine diphosphate-ribosylation (PARylation) was detecyed using Western blotting and immunoprecipitation assays. Results: Pretreatment of the mice with daidzein markedly attenuated TNF-α-induced lung inflammation, and inhibited Cxcl2 expression in lung tissues. Furthermore, daidzein (10 μmol/L) prevented TNF-α-induced increases in Cxcl2 expression and activity and NF-κB transcriptional activity, and markedly inhibited TNF-α-induced protein PARylation in MLE-12 cells in vitro. In MLE-12 cells co-transfected with the PARP-1 expression plasmid and NF-κB-luc (or Cxcl2-luc) reporter plasmid, TNF-α markedly increased NF-κB (or Cxcl2) activation, which were significantly attenuated in the presence of daidzein (or the protein PARylation inhibitor PJ 34). PARP-1 activity assay showed that daidzein (10 μmol/L) reduced the activity of PARP-1 by ~75%. Conclusion: The anti-inflammatory action of daidzein in murine lung epithelial cells seems to be mediated via a direct interaction with PARP-1, which inhibits RelA/p65 protein PARylation required for the transcriptional modulation of pro-inflammatory chemokines such as Cxcl2.

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“…The postprandial blood glucose levels in healthy mice treated with daidzein starch were also significantly lower ( P ≤ 0.05). Similarly, the area under the curve of blood glucose levels in the diabetic mice group fed with starch and daidzein (2043.0 ± 204.9 mmol min L) was significantly lower ( P ≤ 0.05) than the control group (204.2 ± 2475.2 mmol min L) [45]. …”

Section: Genistein and Daidzein: Antidiabetic Activitymentioning

confidence: 99%

The Active Role of Leguminous Plant Components in Type 2 Diabetes

Gętek

Czech

Muc-Wierzgoń

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Diabetes appears to be one of the most frequent noncommunicable diseases in the world. A permanent growth in the incidence of diabetes can be observed and according to the International Diabetes Federation (IDF) the year 2030 will mark the increase in the number of diabetics to 439 mln worldwide. Type 2 diabetes accounts for about 90% of all diabetes incidence. Nutrition model modification not only features the basic element in type 2 diabetes treatment but also constitutes the fundamental factor influencing a morbidity rate decrease. Leguminous plants are a key factor in the diabetic diet; plants such as pulses or soybeans are nutritious products valued highly in nutrition. These legumes are high in the content of wholesome protein and contain large amounts of soluble alimentary fiber fractions, polyunsaturated fatty acids, vitamins and minerals, and bioactive substances with antioxidant, anti-inflammatory, and anticancer activity. They are distinguished by the high amount of bioactive compounds that may interfere with the metabolism of glucose. The most significant bioactive compounds displaying antidiabetic activity in leguminous plants are as follows: genistein and daidzein, alpha-amylase inhibitors, and alpha-glucosidase inhibitors. In vitro research using leguminous plant extracts has confirmed their antidiabetic properties. Leguminous plants should be employed in the promotion of healthy lifestyles in terms of functional food.

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Daidzein inhibits carbohydrate digestive enzymes in vitro and alleviates postprandial hyperglycemia in diabetic mice

Cited by 73 publications

References 43 publications

Effects ofGrifola frondosanon-polar bioactive components on high-fat diet fed and streptozotocin-induced hyperglycemic mice

Effects ofGrifola frondosanon-polar bioactive components on high-fat diet fed and streptozotocin-induced hyperglycemic mice

Daidzein suppresses pro-inflammatory chemokine Cxcl2 transcription in TNF-α-stimulated murine lung epithelial cells via depressing PARP-1 activity

The Active Role of Leguminous Plant Components in Type 2 Diabetes

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