D-Mannose Enhanced Immunomodulation of Periodontal Ligament Stem Cells via Inhibiting IL-6 Secretion

Abstract

Periodontal ligament stem cell- (PDLSC-) mediated periodontal tissue regeneration has recently been proposed for the new therapeutic method to regenerate lost alveolar bone and periodontal ligament. It was reported that both autogenic and allogeneic PDLSCs could reconstruct damaged periodontal tissues but the regeneration effects were not consistent. The effective methods to improve the properties of PDLSCs should be further considered. In this study, we investigated if D-mannose could affect the immunomodulatory properties of hPDLSCs. After being pretreated with D-mannose, hPDLSCs could inhibit T cell proliferation and affect T cell differentiation into Treg cells. We found that less IL-6 could be detected in D-mannose-pretreated hPDLSCs. In the D-mannose pretreatment group, induced Treg cell number would decrease if increased IL-6 levels could be detected. Our data uncovered a previously unrecognized function of D-mannose to regulate the immunomodulatory function of PDLSCs and that IL-6 might play a key role in this process. The results provided a property method to improve PDLSC-based periodontal regeneration.

Inhibition of Tet1- and Tet2-mediated DNA demethylation promotes immunomodulation of periodontal ligament stem cells

“…Next, to evaluate the immunomodulatory properties of PDLSCs, we co-cultured PDLSCs with T cells and found that PDLSCs were capable of inducing T cell apoptosis 16 . Moreover, Tet1/Tet2 siRNA-treated PDLSCs had a significantly elevated capacity to induce AnnexinV + 7AAD + double-positive T cell apoptosis, when compared to the control PDLSCs (Fig.…”

“…MSCs possess great potential for treating immune diseases due to their extensive immunomodulatory properties 16,20 . Whether Tet participates in regulating MSC immunomodulation has remained unknown.…”

D-Mannose Inhibits Adipogenic Differentiation of Adipose Tissue-Derived Stem Cells via the miR669b/MAPK Pathway

“…Besides that, the osteogenic medium contained 2 mM β -glycerophosphate (Sigma-Aldrich, St. Louis, MO), 10 nM dexamethasone (Sigma-Aldrich, St. Louis, MO), and 100 μ M L-ascorbic acid 2-phosphate (Wako Chemicals USA, Richmond, VA). All the medium preparation methods are based on the methods in [ 12 , 15 ] and our previous studies [ 13 ]. Total RNA was extracted from each group with the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) after ten days of inducement, and the gene expression levels of Bglap and Alpl were assayed by real-time PCR analysis.…”

“…Also, other studies confirmed that D-mannose showed the function of immune regulation. A study by Zhang et al showed that D-mannose increased the percentage of regulatory T cells and thus suppressed immunopathology in mouse models of airway inflammation and autoimmune diabetes [ 12 ], and our previous study showed that D-mannose enhanced the immunomodulation function of periodontal ligament stem cells on T cells via inhibiting interleukin- (IL-) 6 secretion [ 13 ]. Besides, D-mannose may be involved in the regulation process of glucose metabolism and fat tissue expansion.…”

Graphene Family Nanomaterials: Properties and Potential Applications in Dentistry

“…Another stem cell from dental tissue that can be influenced by graphene scaffold is periodontal ligament stem cells (PDLSCs). They could be obtained from dental tissue and have shown multiple differentiation capacity and immunomodulation, capable of differentiating into both cementoblasts and collagen forming cells [51]. A study conducted by Xie et al investigated the expression profile of PDLSCs for osteogenic differentiation on two-dimensional graphene (2DGp) and three-dimensional graphene scaffold (3DGp) [52].…”