D-Lactate altered mitochondrial energy production in rat brain and heart but not liver

Ling, Binbing; Peng, Fei; Alcorn, Jane; Lohmann, Katharina L.; Bandy, Brian; Zello, Gordon A.

doi:10.1186/1743-7075-9-6

Cited by 52 publications

(47 citation statements)

References 51 publications

(56 reference statements)

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“…Within the microenvironment of dentin these metabolic products could modulate neurosensory odontoblast activity. Thus, L-lactate is a preferred nutrient for orexin neurons [Parsons and Hirasawa, 2010] while D-lactate can act as a neurotoxin [Ling et al, 2012].…”

Section: Discussionmentioning

confidence: 99%

Glial Network Responses to Polymicrobial Invasion of Dentin

Houshmandi

Hunter

2014

Caries Res

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This study investigated the distribution patterns of glial networks disclosed by reactivity for glial fibrillary acidic protein (GFAP) and S100B in healthy and carious human teeth. The objective was to determine the assembly and collapse of glial networks in response to encroaching infection. 15 healthy and 37 carious posterior teeth from adults were studied. Immediately after extraction, teeth were cleaned and vertically split and the half with pulp fixed and prepared for resin or frozen sections. Sections were stained with toluidine blue and for immunofluorescence, with observation by confocal laser microscopy and analysis by ImageJ software. Carious teeth were subdivided into three groups according to degree of carious involvement: microbial penetration through enamel (stage A), extension into dentin (stage B) and advanced penetration into dentin but without invasion of underlying pulp tissue (stage C). In stage A lesions there was marked increase in glial networks in dental pulp tissue that extended beyond the zone of microbial invasion. This response was maintained in stage B lesions. In advanced stage C lesions these networks were degraded in the zone of invasion in association with failure to contain infection. Cells expressing the glial markers GFAP and S100B showed a response to initial microbial invasion of dentin by increase in number and altered anatomical arrangement. The late stage of dentinal caries was marked by collapse of these networks in the region adjacent to advancing bacteria. This behaviour is important for understanding and explaining the defensive response of the neurosensory peripheral dental pulp apparatus to infection.

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Section: Discussionmentioning

confidence: 99%

Glial Network Responses to Polymicrobial Invasion of Dentin

Houshmandi

Hunter

2014

Caries Res

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“…Rat brain mitochondria were isolated by the differential centrifugation method (Kupsch et al 2007;Ling et al 2012). Briefly, the brain from the adult rat was separated and homogenized by using a mechanically driven Teflon-fitted PotterElvehjem type homogenizer in an ice-cold isolation buffer ( A ) c o n t a i n i n g 2 5 0 m M s u c r o s e , 1 0 m M 4 -( 2 -hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 1 mM EGTA, and 0.1 % fat-free BSA adjusted by Tris to pH 7.4 and centrifuged at 1000g for 8 min at 4°C.…”

Section: Mitochondria Preparationmentioning

confidence: 99%

Neuroprotective activities of curcumin and quercetin with potential relevance to mitochondrial dysfunction induced by oxaliplatin

Waseem

Parvez

2015

Protoplasma

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Peripheral neurotoxicity is one of the serious dose-limiting side effects of oxaliplatin (Oxa) when used in the treatment of malignant conditions. It is documented that it elicits major side effects specifically neurotoxicity due to oxidative stress forcing the patients to limit its clinical use in long-term treatment. Oxidative stress has been proven to be involved in Oxa-induced toxicity including neurotoxicity. The mitochondria have recently emerged as targets for anticancer drugs in various kinds of toxicity including neurotoxicity that can lead to neoplastic disease. However, there is paucity of literature involving the role of the mitochondria in mediating Oxa-induced neurotoxicity and its underlying mechanism is still debatable. The purpose of this study was to investigate the dose-dependent damage caused by Oxa on isolated brain mitochondria under in vitro conditions. The study was also designed to investigate the neuroprotective effects of nutraceuticals, curcumin (CMN), and quercetin (QR) on Oxa-induced mitochondrial oxidative stress and respiratory chain complexes in the brain of rats. Oxidative stress biomarkers, levels of nonenzymatic antioxidants, activities of enzymatic antioxidants, and mitochondrial complexes were evaluated against the neurotoxicity induced by Oxa. Pretreatment with CMN and QR significantly replenished the mitochondrial lipid peroxidation levels and protein carbonyl content induced by Oxa. CMN and QR ameliorated altered nonenzymatic and enzymatic antioxidants and complex enzymes of mitochondria. We conclude that CMN and QR, by attenuating oxidative stress as evident by mitochondrial dysfunction, hold promise as agents that can potentially reduce Oxa-induced adverse effects in the brain.

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“…On one hand, in sepsis, use of substrates and mitochondrial respiration efficiency vary between organs [27]. On the other hand, brain mitochondria may be especially vulnerable to toxic products released as a consequence of septic liver dysfunction [28].…”

Section: Discussionmentioning

confidence: 99%

Time course of blood lactate levels, inflammation, and mitochondrial function in experimental sepsis

et al. 2017

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BackgroundA decrease in blood lactate levels (Lac) >10% during the first hours of resuscitation in sepsis is associated with better outcomes, but the mechanisms are unclear. Our objective was to investigate the relationship between the time course of Lac, inflammatory response, and mitochondrial respiration during experimental sepsis.MethodsOriginal data from two previously published studies were reanalyzed. In cohort 1, pigs were randomized to be resuscitated for 48 h starting at 6, 12, and 24 h, respectively, after fecal peritonitis induction (n = 8 each). Animals were categorized according to the decrease in Lac during the first 6 h of resuscitation (early if ≥10% [Lac ≥10%] or late if <10% or increased [Lac <10%]), and systemic hemodynamics, inflammatory parameters, and mitochondrial function were compared between groups. In a second group of animals with fecal peritonitis and 24 h of resuscitation (n = 16, cohort 2), abdominal regional Lac exchange was measured, and animals were categorized according to the decrease in Lac as in cohort 1.ResultsOverall mortality was 20% (4 of 20) in the Lac ≥10% group and 60% (12 of 20) in the Lac <10% group (p = 0.022). In cohort 1, systemic hemodynamics were similar in the Lac ≥10% (n = 13) and Lac <10% (n = 11) groups. Plasma interleukin-6 levels increased during unresuscitated sepsis and decreased during resusciation in both groups, but they were lower at study end in the Lac ≥10% group (p = 0.047). Complexes I and II maximal (state 3) and resting (state 4) isolated brain mitochondrial respiration at study end was higher in the Lac ≥10% group than in the Lac <10% group, whereas hepatic, myocardial, and skeletal muscle mitochondrial respiration was similar in both groups. In cohort 2, mesenteric, total hepatic, and renal blood flow at study end was higher in the Lac ≥10% group (n = 7) than in the Lac <10% group (n = 9), despite similar cardiac output. Hepatic lactate influx and uptake in the Lac ≥10% group were approximately 1.5 and 3 times higher, respectively, than in the Lac <10% group (p = 0.066 for both).ConclusionsA decrease in Lac >10% during early resuscitation (6 h) after abdominal sepsis is associated with lower levels of plasma interleukin-6 and improved brain but not hepatic or muscle mitochondrial respiration. Blood flow redistribution to abdominal organs in animals with early decrease in Lac concentrations increases the potential to both deliver and extract Lac.Electronic supplementary materialThe online version of this article (doi:10.1186/s13054-017-1691-4) contains supplementary material, which is available to authorized users.

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D-Lactate altered mitochondrial energy production in rat brain and heart but not liver

Cited by 52 publications

References 51 publications

Glial Network Responses to Polymicrobial Invasion of Dentin

Glial Network Responses to Polymicrobial Invasion of Dentin

Neuroprotective activities of curcumin and quercetin with potential relevance to mitochondrial dysfunction induced by oxaliplatin

Time course of blood lactate levels, inflammation, and mitochondrial function in experimental sepsis

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