D-glucosamine as an antagonist of glucose in carbohydrate metabolism of yeast

Woodward, Gladys E.; Hudson, Marie T.

doi:10.1016/0016-0032(53)90316-0

Cited by 13 publications

(4 citation statements)

References 8 publications

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“…Glucosamine is a competitive inhibitor of glucose fermentation (Woodward & Hudson, 1953 the arrows, samples were removed for RNA isolation. (Furst & Michels, 1977), probably due to the competitive inhibition of glucosamine uptake.…”

Section: Introductionmentioning

confidence: 99%

The relationship of growth rate and catabolite repression with WHI2 expression and cell size in Saccharomyces cerevisiae

Mountain

Sudbery

1990

Journal of General Microbiology

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Mixtures of D-glucosamine and glucose were used to slow the growth of wild-type and whi2 mutant strains of Saccharomyces cereuisiae without affecting the level of catabolite repression. The following observations were made. Firstly, mutant cells were found to be partially resistant to the inhibitory effect of glucosamine. Secondly, slow growth induced by glucosamine resulted in cells becoming larger, in direct contrast to the effect of slowing growth by glucose limitation in a chemostat or by carbon source substitution. It is concluded that the level of repression/derepression, rather than absolute growth rate, is responsible for controlling cell size. Thirdly, when WHI2 transcript levels were measured it was found that expression was correlated with growth rate rather than the level of repression. These results are interpreted in terms of a model which envisages that the WHI2 product acts as a negative regulator of catabolite repression. A test of this model is reported: it is shown that mutant cells respired more actively in the presence of glucose and grew more rapidly on glycerol, whereas overexpression of WHZ2 from multicopy plasmids prevented growth on glycerol and depressed respiration.

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Section: Introductionmentioning

confidence: 99%

The relationship of growth rate and catabolite repression with WHI2 expression and cell size in Saccharomyces cerevisiae

Mountain

Sudbery

1990

Journal of General Microbiology

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“…Cucumber explants were cultivated with various concentrations of Glc (0, 0.01, 0.05, 0.10, 0.50 and 1.00 mM). In addition, GlcN as the inhibitor of glucose was used in the study; refer to Woodward [ 39 ] research. The following chemicals were carried out: control (distilled water), 50% HRW, 0.10 mM Glc, 50% HRW + 0.10 mM Glc and 0.10 μM GlcN.…”

Section: Methodsmentioning

confidence: 99%

The Involvement of Glucose in Hydrogen Gas-Medicated Adventitious Rooting in Cucumber

Zhao

Liu

et al. 2021

Plants

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Hydrogen gas (H2) and glucose (Glc) have been reported as novel antioxidants and signal molecules involved in multiple biological processes in plants. However, the physiological roles and relationships of H2 and Glc in adventitious rooting are less clear. Here, we showed that the effects of different concentrations Glc (0, 0.01, 0.05, 0.10, 0.50 and 1.00 mM) on adventitious rooting in cucumber were dose-dependent, with a maximal biological response at 0.10 mM. While, the positive roles of hydrogen rich water (HRW, a H2 donor)-regulated adventitious rooting were blocked by a specific Glc inhibitor glucosamine (GlcN), suggesting that Glc might be responsible for H2-regulated adventitious root development. HRW increased glucose, sucrose, starch and total sugar contents. Glucose-6-phosphate (G6P), fructose-6-phosphate (F6P) and glucose-1-phosphate (G1P) contents were also increased by HRW. Meanwhile, the activities of sucrose-related enzymes incorporating sucrose synthase (SS) and sucrose phosphate synthase (SPS) and glucose-related enzymes including hexokinase (HK), pyruvate kinase (PK) and adenosine 5′-diphosphate pyrophosphorylase (AGPase) were increased by HRW. Moreover, HRW upregulated the expression levels of sucrose or glucose metabolism-related genes including CsSuSy1, CsSuSy6, CsHK1, CsHK3, CsUDP1, CsUDP1-like, CsG6P1 and CsG6P1-like. However, these positive roles were all inhibited by GlcN. Together, H2 might regulate adventitious rooting by promoting glucose metabolism.

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“…A number of glucose analogs have been studied to determine their ability to mimic glucose repression (30,31). Of these, D-glucosamine shows the greatest potential as a gratuitous glucose repressor in yeast and therefore is most useful for the isolation of glucose repressioninsensitive mutants.…”

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confidence: 99%

Pleiotropic glucose repression-resistant mutation in Saccharomyces carlesbergensis

Michels¹,

Romanowski²

1980

J Bacteriol

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We describe the characterization of a mutation of the locus GLRI. This mutation allowed for (i) the glucose repression-insensitive synthesis of the enzymes maltase, galactokinase, a-galactosidase, reduced nicotinamide adenine dinucleotide-cytochrome c reductase, and cytochrome c oxidase and (ii) growth on maltose in the presence of the gratuitous glucose repressor D-glucosamine. The glucosamine resistance cosegregated with the glucose-insensitive synthesis of the enzymes listed above. In addition, crosses between the glucosamine-resistant mutant and isogenic sensitive strains gave only tetrads containing two resistant and two sensitive spores. Thus, a single pleiotropic mutation is responsible for both phenotypes. We call the locus GLR1, for glucose regulation, and the glucose repression-insensitive mutation glrl-1.

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D-glucosamine as an antagonist of glucose in carbohydrate metabolism of yeast

Cited by 13 publications

References 8 publications

The relationship of growth rate and catabolite repression with WHI2 expression and cell size in Saccharomyces cerevisiae

The relationship of growth rate and catabolite repression with WHI2 expression and cell size in Saccharomyces cerevisiae

The Involvement of Glucose in Hydrogen Gas-Medicated Adventitious Rooting in Cucumber

Pleiotropic glucose repression-resistant mutation in Saccharomyces carlesbergensis

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