d-Glucans from edible mushrooms: A review on the extraction, purification and chemical characterization approaches

Ruthes, Andréa Caroline; Smiderle, Fhernanda Ribeiro; Iacomini, Marcello

doi:10.1016/j.carbpol.2014.10.051

Cited by 156 publications

(69 citation statements)

References 73 publications

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“…For preliminary characterization of the monomer types present in Rusalan, HPTLC was performed followed by orcinol-H 2 SO 4 spray. The reagent reacts differently to each monosaccharide showing a specific colour and colour intensities vary depending on sugar concentration [59]. In the present study, the chromatogram of Rusalan demonstrated to be consisted of three monosaccharides i.e.…”

Section: Resultsmentioning

confidence: 63%

Introducing a novel mushroom from mycophagy community with emphasis on biomedical potency

et al. 2017

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Mushrooms have been prized by humankind as medicine and culinary wonder since antiquity. Though several species are ethnically valued; many prospective species are still being discovered. One such wild macrofungus has recently been discovered during subsequent field surveys in West Bengal, India which in turn exposed as a traditionally consumed popular myco-food. The collected taxon was found to be unique with regard to its morphological as well as genetical features. After detailed characterizations, the fungus was identified as a novel taxon belonging to the genus Russula (Russulaceae, Basidiomycota). Besides, the investigation was further extended in search of new functional ingredients and in this context, a water soluble crude polysaccharide rich extract (Rusalan) was isolated from dried basidiocarps. Accumulating evidences from GC-MS, HPTLC, FT-IR along with several spectrophotometric methods postulated that the fraction consisted mainly of carbohydrate in triple helical conformation, where glucose was the major monosaccharide mostly with β-type glycosidic linkage. Conversely, Rusalan showed pronounced antioxidant activity in six in vitro assay systems with EC50 value ranging from 190–1328 μg/ml concentration. The crude polysaccharide was also evaluated against six bacterial strains using microdilution method and the growth of Staphylococcus aureus and Bacillus subtilis were found to be inhibited effectively. In addition, immune-stimulatory assays demonstrated that Rusalan could evidently promote proliferation, induce phagocytosis, release NO, produce intracellular ROS and upregulate mRNA expression of iNOS, TNF-α, COX-2, as well as IL-6 genes in in mouse macrophage cells. Therefore, aim of the present study was not only to describe a new taxon to the world mycoflora but also to introduce a potent therapeutic agent that could be explored for food and pharmaceutical purposes. However, isolation of active component and in vivo studies need to be designed further.

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Section: Resultsmentioning

confidence: 63%

Introducing a novel mushroom from mycophagy community with emphasis on biomedical potency

et al. 2017

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“…The cold water-soluble fraction (SK2-Ab) was treated with Fehling solution (Ruthes et al, 2015). The Fehling-soluble fraction (FSK2-Ab) was isolated from the insoluble Cu 2+ complex (FPK2-Ab) by centrifugation (8000 rpm, at 5 ºC, for 20 min), neutralized with acetic acid, dialyzed against tap water, deionized with mixed-ion exchange resins and freeze dried.…”

Section: Reagentsmentioning

confidence: 99%

“…Fractionation and purification of K2-Ab (18.4 g) was carried out with a freeze-thawing procedure (Ruthes et al, 2015), yielding a cold water-soluble fraction (SK2-Ab; 6.0 g, 32.6% yield). Showing a heterogeneous elution profile by HPSEC, fraction SK2-Ab was further fractionated by treatment with Fehling solution followed by closed dialysis of the copper-complex fraction, FPK2-Ab (1.1 g, 6.0% yield), through a 100 kDa MWCO membrane (Fig.…”

Section: Structural Characterization Of the Mannogalactoglucanmentioning

confidence: 99%

Cytotoxic effect of a mannogalactoglucan extracted from Agaricus bisporus on HepG2 cells

Pires

Ruthes

Cadena

et al. 2017

Carbohydrate Polymers

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“…Depending on the fungi culture conditions (submerged or solid culture, pH, temperature, oxygen concentration, composition of the culture medium etc) and of the method of extraction, polysaccharides with different structures and, consequently, different bioactivities can be obtained from the fruiting bodies, from the fermented culture broth or from the mycelial biomass (Zhang et al 2007, Synytsya and Novák 2013, Ruthes et al 2015. Silveira et al (2015) reported a mannogalactan with antinociceptive and antiinflammatory effects extracted from the culture broth of Pleurotus sajor-caju by freeze-thawing and dialysis.…”

Section: Introductionmentioning

confidence: 99%

A polysaccharide fraction extracted from Pleurotus ostreatus mycelial biomass inhibit Sarcoma 180 tumor

Wisbeck

Facchini

Alves

et al. 2017

An. Acad. Bras. Ciênc.

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Fungi of Pleurotus genus have attracted a great interest due to their medicinal properties such as antiinflammatory, antimicrobial and antitumor. These properties are attributed mainly to polysaccharides synthesized by Pleurotus. This work aimed to study the mycelial growth of P. ostreatus in submerged culture, evaluating the influence of the initial concentration of substrate (20 and 40 g/L of glucose) and the pH (4 and 6) on kinetic parameters of production of biomass. The effectiveness of different doses (10, 30 and 50 mg/kg) of a mycelium polysaccharide fraction extracted from P. ostreatus in reducing Sarcoma 180 development in mice was also verified. In the range of this study, maximum concentration of mycelial biomass (about 12.8 g/L) was obtained using 40.0 g/L of glucose, at pH 4.0. The total biomass productivity (Px) was not significantly affected by substrate concentration and pH, reaching values of 0.034 g/L.h. Sarcoma 180 tumor weight was reduced in 74.1, 75.5 and 53.7% when 10, 30 and 50 mg/kg were administered, respectively. These results show the high antitumor potential of intracellular polysaccharide fraction of mycelial biomass of P. ostreatus, particularly at lower doses of 10 and 30 mg/kg.

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d-Glucans from edible mushrooms: A review on the extraction, purification and chemical characterization approaches

Cited by 156 publications

References 73 publications

Introducing a novel mushroom from mycophagy community with emphasis on biomedical potency

Introducing a novel mushroom from mycophagy community with emphasis on biomedical potency

Cytotoxic effect of a mannogalactoglucan extracted from Agaricus bisporus on HepG2 cells

A polysaccharide fraction extracted from Pleurotus ostreatus mycelial biomass inhibit Sarcoma 180 tumor

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