D‐galactose‐induced toxicity associated senescence mitigated by alpinate oxyphyllae fructus fortified adipose‐derived mesenchymal stem cells

Abstract

This study addresses the effect of D-galactose-induced toxicity associated senescence mitigated by alpinate oxyphyllae fructus (AOF; Alpinia oxyphylla Miq) extracts fortified with adipose-derived mesenchymal stem cells (ADMSCs) in rats. Male 18 week-old Wistar Kyoto (WKY) rats were used in this study. We analyzed cardiac fibrosis by Masson's trichrome staining. The tissue sections were dyed using hematoxylin and eosin (H&E). Tissue sections were stained for the restoration of Nrf2 expression in treatment groups by immunohistochemistry. Immunohistochemistry and western blotting analysis showed that AOF with ADMSCs could significantly reduce aging-induced oxidative stress in D-galactose-induced aging rat hearts by inducing Nrf2 pathway. Reduction in ROS resulted in the suppression of inflammatory signals (p-NF-κB and IL-6). Histopathological studies were showed an increased interstitium and collagen accumulation in aging-induced heart sections. However, AOF and ADMSCs treated hearts were recovered from cardiac remodeling. Furthermore, hypertrophy and fibrosis associated markers were also significantly reduced (P < .05) in treatment groups. We speculate that ADMSCs might activate certain Wei-Wen Kuo and Chih-Yang Huang have contributed equally.

miR‐222‐5p promotes dysfunction of human vascular smooth muscle cells by targeting RB1

“…Next, the samples were fixed with paraformaldehyde (4%) and stained with collagen fibers (blue staining) using Masson's trichrome method (Sigma-Aldrich). 24 Next, the lesion size was photographed and analyzed under a polarized light microscope (Olympus).…”

Human adipose and synovial mesenchymal stem cells improve osteoarthritis in rats by reducing chondrocyte reactive oxygen species and inhibiting inflammatory response

“…Inflammation could cause oxidative stress, and the reduction of ROS, a specific indicator of oxidative stress, led to the suppression of inflammatory signals (P‐NF ‐κB and IL‐6). 19 Therefore, we further examined ROS level in IL‐1β‐induced cells to figure out the interaction between oxidative stress and inflammatory response in the progression of arthritis. Then, the ROS level in each group was detected by the ROS kit.…”

“…That is, IL‐1β inhibited the expressions of COL2A1, Aggrecan and SOX9, but promoted the expression of MMP‐13 (Figure 1A, p < 0.001). Inflammation could cause oxidative stress, and the reduction of ROS, a specific indicator of oxidative stress, led to the suppression of inflammatory signals (P‐NF ‐κB and IL‐6) 19 . Therefore, we further examined ROS level in IL‐1β‐induced cells to figure out the interaction between oxidative stress and inflammatory response in the progression of arthritis.…”

Cardioprotective effects of transplanted adipose‐derived stem cells under Ang II stress with Danggui administration augments cardiac function through upregulation of insulin‐like growth factor 1 receptor in late‐stage hypertension rats

“…MSCs can be isolated from various kinds of tissues, including adipose tissue, bone marrow, Wharton's Jelly, liver, brain, umbilical cord, and placenta 10 . The microenvironment as well as autocrine and paracrine activity of MSCs help to maintain their proliferation and stemness 11,12 . MSCs have also the properties to exert angiogenesis and anti‐inflammatory factors after the transplantation to the host body 13 .…”

“…10 The microenvironment as well as autocrine and paracrine activity of MSCs help to maintain their proliferation and stemness. 11,12 MSCs have also the properties to exert angiogenesis and anti-inflammatory factors after the transplantation to the host body. 13 Bone marrow MSCs treatment is known to develop the ejection fraction and left ventricular volumes in myocardial infarction patients.…”