D-Galactose Induced Changes in Enzymatic Antioxidant Status in Rats of Different Ages

Abstract

Considering the preexisting influence of the process of natural aging on antioxidant enzymes activity and the level of lipid peroxidation, the age of the rats at which D-galactose (D-gal) treatment is started could strongly impact the development of D-gal induced senescence. To evaluate this, we subjected 1, 3 and 15 months old rats to D-gal treatment in parallel with having appropriate placebos (0.9 % saline). Our results showed elevated glutathione peroxidase (GPx) activity and no significant changes in superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) activity or malondialdehyde (MDA) levels in relation to natural aging. In mature and aged senescent livers we observed positive correlation between increased ratio R=SOD/(GPx+CAT) and increased MDA concentration. MDA levels seemed to correlate positively with the age of the animals at which D-gal treatment had started. In the case of 3 and 15 months old rats there was D-gal induced decrease in SOD and GR activity, but this effect of the treatment was not observed in 1 month old rats. Our results imply that the changes in the antioxidant enzyme activities are not only under the influence of the D-gal overload, but also depend on the developmental stage of the rats. According to our results, with regard to enzymatic antioxidant capacity and the level of lipid peroxidation, the best age for induction of senescence is somewhere after the third month.

Attenuation of Age-Related Hepatic Steatosis by Dunaliella salina Microalgae in Senescence Rats through the Regulation of Redox Status, Inflammatory Indices, and Apoptotic Biomarkers

“…All of these results have been confirmed by the hepatic histopathological examination as dilatation and congestion were detected in the central as well as the portal veins with inflammatory cells infiltration in the portal zone and degeneration in the hepatocytes all over the parenchyma. ese findings were in accordance with [38,38]. Treatment with D. salina carotenoids showed amelioration of the histopathological examination of the liver tissues exhibiting almost Advances in Pharmacological and Pharmaceutical Sciences normal hepatic lobules with an average sized central vein, while zeaxanthin showed thick-walled attenuated blood vessels with preserved hepatic architecture than appeared sections isolated from rats treated with polar fractions.…”

Hypoxia induces senescence of bone marrow mesenchymal stem cells via altered gut microbiota

“…4B ). d -galactose has been extensively leveraged in mouse models of ageing 32 , 33 . Therefore, the accumulated excessive d -galactose in BM could exert a negative effect on BMSCs.…”

“…High-dose d -galactose can induce premature ageing of astrocytes, foetal fibroblasts and neuroblasts 32 , 34 . A number of reports have suggested that the ageing model induced by d -galactose is associated with oxidative stress 33 . Subsequent studies have demonstrated that ROS accumulation mainly accounts for this process 23 .…”

C-phycocyanin protects against low fertility by inhibiting reactive oxygen species in aging mice

“…D-gal-induced aging is associated with oxidative stress and AGE generation [ 43 ]. ROS play key roles in this process as their accumulation leads to the induction and maintenance of cellular senescence [ 44 ].…”