C-phycocyanin protects against low fertility by inhibiting reactive oxygen species in aging mice

Li, Yanjiao; Han, Zhe; Ge, Liang; Zhou, Cheng-Jie; Zhao, Yuefang; Wang, Donghui; Ren, Jing; Niu, Xin-Xin; Liang, Cheng-Guang

doi:10.18632/oncotarget.8165

Cited by 55 publications

(34 citation statements)

References 63 publications

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“…d ‐gal injections had been reported to cause liver damage and dysfunction, which would lead to an overall morphological change, as well as increase levels or activities of some serum enzymes (Li et al., ). This study showed that ALT and AST levels were significantly elevated in mouse livers injected with d ‐gal for 8 weeks.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of walnut on d‐galactose‐induced aging mouse model

Liu

Chen²,

Wang

et al. 2019

Food Science & Nutrition

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Objective (s) Accumulating evidence has suggested that oxidative stress and apoptosis are involved in the aging process. d ‐galactose (gal) has been reported to cause symptoms of aging in mice, accompanied by liver and brain injuries. Our present work was to study the potential antioxidative and anti‐apoptotic effects of walnut and to explore how these effects act on mice in a d ‐gal‐induced aging model. Materials and Methods Aging mice were induced by subcutaneous injection of d ‐gal (200 mg kg −1 d −1 for 8 weeks). Walnut samples were simultaneously administered to the d ‐gal‐induced aging mice once daily by intragastric gavage. Finally, body weight, organ index, cognitive function, levels of antioxidative enzymes, and liver function were monitored. Results The kernel pellicles of walnut could not only improve the learning and memory ability, and the organ index, but also significantly decrease body weight and normalize the levels of activity of antioxidative enzymes in aging mice. Further, the walnut seed coat would protect damages of hippocampus and liver in aging mice. Highlights In the current study, we investigated the effects of walnut kernels and walnut seed coats (WSCs) on d ‐galactose‐induced aging mice. WSC was firstly found to have beneficial effects on d ‐gal‐treated mouse's brain with learning and memory impairment, which probably through the underlying mechanism reduces oxidative damage and limits neuroinflammation. In addition, WSC had a protective effect on liver damage in d ‐galactose sensing mice.

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Section: Discussionmentioning

confidence: 99%

Protective effect of walnut on d‐galactose‐induced aging mouse model

Liu

Chen²,

Wang

et al. 2019

Food Science & Nutrition

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“…13 It has been indicated that C-phycocyanin has expressed anti-inflammatory, antioxidant and radical scavenging properties. 14 In an aspirin-induced gastric ulcer model, spirulina has shown ameliorative effects by alleviating oxidative stress and inflammation. 15 Thus, it is intriguing to ascertain the protective effect of C-phycocyanin in a well-established model of gastric ulcer.…”

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confidence: 99%

C‐phycocyanin protects against ethanol‐induced gastric ulcers in rats: Role of HMGB1/NLRP3/NF‐κB pathway

Alzokaky

Abdelkader

El-Dessouki

et al. 2020

Basic Clin Pharma Tox

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Gastric ulcer is a widespread inflammatory disease with high socio‐economic burden. C‐phycocyanin is one of the active constituents of Spirulina microalgae, and although it is well known for its antioxidant and anti‐inflammatory properties, its protective effects against gastric ulcer have not yet been identified. High‐mobility group box 1 (HMGB1) is a nuclear protein that, once secreted extracellularly, initiates several inflammatory reactions, and it is involved in the pathogenesis of gastric ulcer. The aim of the present study was to investigate the anti‐inflammatory and anti‐ulcerogenic effects of C‐phycocyanin against ethanol‐induced gastric ulcer targeting HMGB1/NLRP3/NF‐κB pathway. Ulcer induction showed increase in HMGB1 expression through activation of nucleotide‐binding domain and leucine‐rich repeat‐containing protein 3 (NLRP3) inflammasome and nuclear factor kappa p65 (NF‐κB p65). Moreover, oxidative stress and inflammatory markers were elevated in the ulcer‐treated group compared to the normal control group. However, pre‐treatment with C‐phycocyanin significantly reduced HMGB1 expression via suppression of NLRP3/NF‐κB, oxidative markers, IL‐1β, tumour necrosis factor‐α (TNF‐α) and ulcer index value. These results were consistent with histopathological and immunohistochemistry examination. Thus, C‐phycocyanin is a potential therapeutic strategy with anti‐inflammatory and anti‐ulcerogenic effects against ethanol‐induced gastric ulcer.

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“…Oocytes treated with a single MAM reagent had larger PB1, this was consistent with previous results showing that the single use of dbcAMP, IBMX, or forskolin generated abnormal PB1 due to failed spindle migration (Chen et al, 2012). A high incidence of apoptosis is associated with a reduction in oocyte quality (Li et al, 2016; Wu et al, 2017). We confirmed that the single use of Mil or forskolin caused a higher ratio of apoptosis, suggesting that oocytes incubated with a high concentration of MAM reagents may be of lower quality.…”

Section: Discussionmentioning

confidence: 99%

Combined use of dbcAMP and IBMX minimizes the damage induced by a long‐term artificial meiotic arrest in mouse germinal vesicle oocytes

Han

Hao

et al. 2020

Molecular Reproduction Devel

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Phosphodiesterase (PDE)‐mediated reduction of cyclic adenosine monophosphate (cAMP) activity can initiate germinal vesicle (GV) breakdown in mammalian oocytes. It is crucial to maintain oocytes at the GV stage for a long period to analyze meiotic resumption in vitro. Meiotic resumption can be reversibly inhibited in isolated oocytes by cAMP modulator forskolin, cAMP analog dibutyryl cAMP (dbcAMP), or PDE inhibitors, milrinone (Mil), Cilostazol (CLZ), and 3‐isobutyl‐1‐methylxanthine (IBMX). However, these chemicals negatively affect oocyte development and maturation when used independently. Here, we used ICR mice to develop a model that could maintain GV‐stage arrest with minimal toxic effects on subsequent oocyte and embryonic development. We identified optimal concentrations of forskolin, dbcAMP, Mil, CLZ, IBMX, and their combinations for inhibiting oocyte meiotic resumption. Adverse effects were assessed according to subsequent development potential, including meiotic resumption after washout, first polar body extrusion, early apoptosis, double‐strand DNA breaks, mitochondrial distribution, adenosine triphosphate levels, and embryonic development. Incubation with a combination of 50.0 μM dbcAMP and 10.0 μM IBMX efficiently inhibited meiotic resumption in GV‐stage oocytes, with low toxicity on subsequent oocyte maturation and embryonic development. This work proposes a novel method with reduced toxicity to effectively arrest and maintain mouse oocytes at the GV stage.

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C-phycocyanin protects against low fertility by inhibiting reactive oxygen species in aging mice

Cited by 55 publications

References 63 publications

Protective effect of walnut on d‐galactose‐induced aging mouse model

Protective effect of walnut on d‐galactose‐induced aging mouse model

C‐phycocyanin protects against ethanol‐induced gastric ulcers in rats: Role of HMGB1/NLRP3/NF‐κB pathway

Combined use of dbcAMP and IBMX minimizes the damage induced by a long‐term artificial meiotic arrest in mouse germinal vesicle oocytes

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