Cytotoxicity, Uptake Behaviors, and Oral Absorption of Food Grade Calcium Carbonate Nanomaterials

Kim, Mi-Kyung; Lee, Jeong-A; Jo, Mi-Rae; Kim, Minkyu; Kim, Hyoung-Mi; Oh, Jae‐Min; Song, Nam Woong; Choi, Soo‐Jin

doi:10.3390/nano5041938

Cited by 43 publications

(27 citation statements)

References 31 publications

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“…An in vitro model of human intestinal FAE, representing M cells, was prepared according to the protocol developed by des Rieux et al [ 45 ]. Human intestinal epithelial Caco-2 cells were purchased from the Korean Cell Line Bank (Seoul, Korea) and grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum, 1% non-essential amino acids, 1% l -glutamine, 100 units/mL penicillin, and 100 μg/mL streptomycin under the standard condition as described elsewhere [ 46 ]. Briefly, Transwell ® polycarbonate inserts (Corning Costar, New York, NY, USA) were coated with Matrigel TM basement membrane matrix (Becton Dicknson, Bedford, MA, USA), prepared in pure DMEM, and were then placed at room temperature for 1 h. The supernatants were removed and the inserts were washed with DMEM.…”

Section: Methodsmentioning

confidence: 99%

Titanium Dioxide Nanoparticle-Biomolecule Interactions Influence Oral Absorption

Jin

Kim

et al. 2016

Nanomaterials

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Titanium dioxide (TiO2) nanoparticles (NPs) have been widely applied in various industrial fields, such as electronics, packaging, food, and cosmetics. Accordingly, concerns about the potential toxicity of TiO2 NPs have increased. In order to comprehend their in vivo behavior and potential toxicity, we must evaluate the interactions between TiO2 NPs and biomolecules, which can alter the physicochemical properties and the fate of NPs under physiological conditions. In the present study, in vivo solubility, oral absorption, tissue distribution, and excretion kinetics of food grade TiO2 (f-TiO2) NPs were evaluated following a single-dose oral administration to rats and were compared to those of general grade TiO2 (g-TiO2) NPs. The effect of the interactions between the TiO2 NPs and biomolecules, such as glucose and albumin, on oral absorption was also investigated, with the aim of determining the surface interactions between them. The intestinal transport pathway was also assessed using 3-dimensional culture systems. The results demonstrate that slightly higher oral absorption of f-TiO2 NPs compared to g-TiO2 NPs could be related to their intestinal transport mechanism by microfold (M) cells, however, most of the NPs were eliminated through the feces. Moreover, the biokinetics of f-TiO2 NPs was highly dependent on their interaction with biomolecules, and the dispersibility was affected by modified surface chemistry.

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Section: Methodsmentioning

confidence: 99%

Titanium Dioxide Nanoparticle-Biomolecule Interactions Influence Oral Absorption

Jin

Kim

et al. 2016

Nanomaterials

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“…The studied compounds consist of an arylpiperazine subunit that is considered to be a privileged chemical structure because it can be found in a variety of pharmacologically interesting compounds capable of interacting with various counterpart cellular targets . Some of the arylpiperazines that are in use in clinics have neuroprotective activity . In our previous publications, we already described neuroprotective properties of N ‐{[2‐(4‐phenyl‐piperazin‐1‐yl)‐ethyl]‐phenyl}‐arylamides …”

Section: Resultsmentioning

confidence: 99%

Identification of NQO1 and ferrochelatase as interaction partners for neuroprotective N‐{[2‐(4‐phenyl‐piperazin‐1‐yl)‐ethyl]‐phenyl}‐arylamides

et al. 2018

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Affinity chromatography was used to identify potential cellular targets that are responsible for neuroprotective activity of N-{[2-(4-phenyl-piperazin-1-yl)-ethyl]-phenyl}-arylamides. Active and inactive representatives of N-{[2-(4-phenyl-piperazin-1-yl)-ethyl]-phenyl}-arylamides bearing an extended linker were synthesized and immobilized on an agarose-based matrix. This was followed by the identification of specifically bound proteins isolated out of the whole rat brain extract. Inducible flavoprotein NAD(P)H:quinone oxidoreductase (NQO1) was identified as candidates for cellular targets.

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“…This means that the CC particles were more cytotoxic than the CC-stabilized emulsion. This difference may be related to the surface roughness of the CC particles, which can minimize the repulsive forces between CC particles and the plasma membrane, inducing membrane damage or cellular uptake [36]. This effect was decreased in the stabilized emulsion, where the CC particles were inside the emulsion structure.…”

Section: In Vitro Cytotoxicity Assaysmentioning

confidence: 99%

Pickering Emulsions Stabilized by Calcium Carbonate Particles: A New Topical Formulation

et al. 2020

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Pickering emulsions are systems composed of two immiscible fluids stabilized by solid organic or inorganic particles. Pickering emulsions are particularly useful in cosmetics, where the surfactants are unwanted, as well as in the pharmaceutical field, where transdermal and/or dermal drug delivery is difficult to achieve and controlled drug release is desired. Here, we studied calcium carbonate particles as stabilizers of Pickering emulsions for topical use. An optimized formulation was obtained using a Quality by Design approach. First, a screening experiment was performed to identify the formulation and process critical variables that affect the quality properties of the Pickering emulsion. The optimization of the production was then studied by establishing the design space. The final formulation was hereinafter investigated regarding the pH, rheological properties, and in vitro cytotoxicity assays. The results showed the formulation had a pH compatible with human skin and a shear thinning behavior. Moreover, this formulation showed a strong network structure, with a suitable spreadability on the skin, allowing an easy application. The in vitro assays were performed to assess the potential cytotoxicity of the calcium carbonate-stabilized emulsion and the particles themselves, and the results revealed that the formulation did not significantly affect the cell viability. In conclusion, the use of calcium carbonate particles as a stabilizer ingredient contributed to achieve an eco-friendly Pickering emulsion.

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Cytotoxicity, Uptake Behaviors, and Oral Absorption of Food Grade Calcium Carbonate Nanomaterials

Cited by 43 publications

References 31 publications

Titanium Dioxide Nanoparticle-Biomolecule Interactions Influence Oral Absorption

Titanium Dioxide Nanoparticle-Biomolecule Interactions Influence Oral Absorption

Identification of NQO1 and ferrochelatase as interaction partners for neuroprotective N‐{[2‐(4‐phenyl‐piperazin‐1‐yl)‐ethyl]‐phenyl}‐arylamides

Pickering Emulsions Stabilized by Calcium Carbonate Particles: A New Topical Formulation

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