Cytotoxicity Structuralmetabolitic Complexes of Lactobacillus Rhamnosus Gg and Saccharomyces Boulardii

Isayenko, O. Yu.; Knysh, O. V.; Falko, Oksana V.; Prokopіuk, Volodymyr; Prokopyuk, O.S.; Zholobak, Nadezhda

doi:10.15407/fz65.05.040

Cited by 4 publications

(4 citation statements)

References 9 publications

(9 reference statements)

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“…tox + and the results presented regarding the potentiation of the antimicrobial activity of antibacterials by the filtrates of L. rhamnosus GG and S. boulardii and increased sensitivity of Corynebacterium spp. to their combined application Isajenko et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Obtaining metabolites (structural-metabolites substances) of bacteria and fungi in disintegrates of probiotic cultures: sample ML-1 and ML-10 -metabolites of Lactobacillus were obtained as a result of cultivation of suspensions of L. rhamnosus GG with optical density of 1.0 and 10.0 units according to McF scale, respectively, in their own disintegrates (Isajenko et al, 2019), sample MS-1 and MS-10 -metabolites of Saccharomyces were obtained by growing a suspension of S. boulardii with optical density of 1.0 and 10.0 units according to McF scale, respectively, in the mushroom's own disintegrates, sample LS -metabolites of saccharomyces, different from the previous ones, were obtained by cultivation of mushroom suspensions in disintegrates of L. rhamnosus GG; sample MLS-1 and MLS-10 -a combination of metabolites of Lactobacillus and Saccharomyces, was obtained by the joint cultivation of microbial cells of L. rhamnosus GG and S. boulardii with optical density of 1.0 and 10.0 units according to McF scale, respectively, in disintegrates of lactobacteria (Isajenko et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

“…Obtaining metabolites of bacteria and fungi in a liquid nutrient medium: sample ML-1 (b) and ML-10 (b) -metabolites of Lactobacillus, was obtained as a result of cultivation of suspensions of L. rhamnosus GG with optical density of 1.0 and 10.0 units according to McF scale, respectively, in a liquid nutrient medium, in particular, in nutrient broth with the addition of 1% glucose (Isajenko et al, 2019); sample МS-1 (b) and МS-10 (b) -metabolites of Saccharomyces, was obtained as a result of cultivation of suspensions of S. boulardii with optical density of 1.0 and 10.0 units according to McF scale, respectively, in a liquid nutrient medium, in particular, in nutrient broth with the addition of 1% glucose; sample МLS-1 (b) and МLS-10 (b) -a combination of metabolites of Lactobacillus and Saccharomyces, obtained by the joint cultivation of microbial cells of L. rhamnosus GG and S. boulardii with optical density of 1.0 and 10.0 units according to McF scale, respectively, in a liquid nutrient medium, in particular, in nutrient broth with the addition of 1% glucose (Isajenko et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

“…Biologically active substances of probiotic origin, as alternative methods for the treatment of infectious diseases, are taking leading positions. They have several advantages: they are highly active, safe, have a broad spectrum of antimicrobial action, have low resistance rates, predominantly exhibit immunomodulatory properties, promote healing of infectious processes of different genesis and etiology (Berditsch et al, 2015;Thomsen et al, 2016;Isajenko et al, 2019). The combined approach is distinguished by the use of two or more therapies for the treatment of infectious diseases.…”

Section: Introductionmentioning

confidence: 99%

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Synergistic activity of filtrates of Lactobacillus rhamnosus and Saccharomyces boulardii and antibacterial preparations against Corynebacterium spp.

Isayenko

2019

Regul. Mech. Biosyst.

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We present the results of the first study of the combined influence of the biologically active substances Lactobacillus rhamnosus GG ATCC 53103 and Saccharomyces boulardii, obtained by the author’s method, and antibacterial agents on Corynebacterium spp. The first area of research was the study of increasing the sensitivity of toxigenic microorganisms to antimicrobial drugs due to the consecutive effects of the structural components and metabolites of L. rhamnosus GG and S. boulardii and antibacterial drugs on Corynebacterium spp. tox+. The greatest increase in the sensitivity of test-cultures of corynebacteria to penicillin (by 19.4 mm), imipenem (by 15.0 mm), vancomycin (by 12.0 mm), gentamicin (by 11.0 mm), ciprofloxacin (by 9.8 mm), erythromycin (by 9.6 mm), cefotaxime (by 9.5 mm) occurred due to the products of lactobacteria and a combination of metabolites of lactobacteria and saccharomycetes. The second area of research was the study of the synergic activity of substances L. rhamnosus GG and S. boulardii and traditional antibacterial drugs manifested by their simultaneous effect on Corynebacterium spp. Maximum potentiation of azithromycin (by 4.6 mm), erythromycin (by 4.5 mm), cefotaxime (by 2.2 mm), ceftriaxone (by 1.6 mm) and ampicillin (by 1.0 mm) relative to corynebacteria was also observed under the influence of lactobacteria metabolites and a combination of lactobacteria and saccharomycetes metabolites. Different degrees of manifestation of the combined action of biologically active substances L. rhamnosus GG and S. boulardii with antibiotics were determined, which depended on the selected combinations, the method of influence on the microorganism, the individual sensitivity of the test-cultures, the activity of the test filtrates and the initial concentration of the producers used to obtain the products of vital activity of lactobacteria and saccharomyces. The presented complexes of structural components and metabolites of L. rhamnosus GG and S. boulardii, obtained without the use of traditional nutrient media, by increasing the bioavailability of pathogenic pathogens can reduce the required concentration of the antibiotic, continuing their use, and suspend the likelihood of pathogens developing resistance to microorganisms. This makes them promising candidates both for the development of "accompaniment-preparations" for antibiotics for the additional therapy of infectious diseases of different etiology, and for the creation of a new direction of antimicrobial agents with multifunctional capabilities. Synergistic activity of filtrates L. rhamnosus GG and S. boulardii and antibacterial preparations against Corynebacterium spp.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synergistic activity of filtrates of Lactobacillus rhamnosus and Saccharomyces boulardii and antibacterial preparations against Corynebacterium spp.

Isayenko

2019

Regul. Mech. Biosyst.

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Anti–diphtheria Properties of Structural–metabolites Complexes of Lactobacteria and Saccharomyces Probiotic Strains

Isayenko¹

2019

Fiziol Zh

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The effectiveness of local action of nano oxides on wound infection

Popadyuk,

Polyovyy,

Henyk

et al. 2024

Fiziol Zh

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The treatment of wound-related combat injuries and antibiotic resistance require the search for new approaches to reduce the risk of infectious complications, this will increase the effectiveness of therapy and even save lives. The aim of our study was to establish the mechanism of action and effectiveness of nano oxides applied to wound infection. The antimicrobial activity of different concentrations of solutions of zinc (nZnO), magnesium (nMgO) and silicon (nSiO2) nano oxides was studied by agar diffusion against clinical antibiotic-sensitive strains of opportunistic Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), isolated from patients with purulent-septic diseases. It was found that nZnO exhibits extremely high antibacterial activity against S. aureus, E. сoli. The ultra-low content of nZnO (1.56 mg/ml) completely destroys S. aureus, unlike nMgO, the effective concentration of which exceeds the minimum by 16 times. It is known that nZnO has the highest antimicrobial properties against S. aureus and E. coli, this effectiveness is achieved due to the features of the structure and the possibility of the formation of oxygen and a number of other radicals. These qualities of nZnO make it possible to integrate it into dressings for wound treatment.

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Cytotoxicity Structuralmetabolitic Complexes of Lactobacillus Rhamnosus Gg and Saccharomyces Boulardii

Cited by 4 publications

References 9 publications

Synergistic activity of filtrates of Lactobacillus rhamnosus and Saccharomyces boulardii and antibacterial preparations against Corynebacterium spp.

Synergistic activity of filtrates of Lactobacillus rhamnosus and Saccharomyces boulardii and antibacterial preparations against Corynebacterium spp.

Anti–diphtheria Properties of Structural–metabolites Complexes of Lactobacteria and Saccharomyces Probiotic Strains

The effectiveness of local action of nano oxides on wound infection

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