Cytotoxicity of α-terpineol in HeLa cell line and its effects to apoptosis and cell cycle

Noor, Rasuane; Astuti, Indwiani; Mustofa, .

doi:10.19106/jmedscie004601201401

Cited by 13 publications

(10 citation statements)

References 11 publications

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“…However, the highest cumulative drug release from H-BSA300-0.03M at pH 6.4 was equal to 123.6 ± 1.7 μg, which still slightly exceeds the DOX concentration of 100 μg, which has been previously reported to effectively kill HeLa cells. 33 Such results therefore suggest that the injectable hydrogel can be tuned to provide appropriate drug release profiles for controlled drug release, as seen by H-BSA300-0.03M. The overall results suggest that the physical properties and the drug release profiles were essentially governed by the supramolecular interactions underpinning the hydrogel formation, as summarized and illustrated in Scheme 1 .…”

Section: Resultsmentioning

confidence: 85%

Injectable Poly(ethylene glycol) Hydrogels Cross-Linked by Metal–Phenolic Complex and Albumin for Controlled Drug Release

2020

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Injectable hydrogel is advantageous as a drug reservoir for controlled drug release since its injectability provides minimally invasive access to internal tissues and irregular-shaped target sites. Herein, we fabricated pH-responsive injectable hydrogels constructed of a supramolecular cross-link network, which contained tannic acid (TA), Fe(III), poly(ethylene glycol) (PEG), and bovine serum albumin (BSA) for controlled drug release. The hydrogel precursors rapidly turned into a gel when co-injected with NaOH in a time scale of seconds. The hydrogel properties and drug release profiles are all tunable by adjusting the concentrations of BSA, NaOH, and doxorubicin (DOX). The Young’s moduli range from 3.19 ± 0.93 to 43.24 ± 1.37 kPa that match internal soft tissues. The hydrogel lasts more than 3 weeks and gradually releases doxorubicin up to 123.6 ± 1.7 μg at pH 6.4. The results of the physical properties and drug release suggest supramolecular interactions that correspond to Fourier transform infrared (FTIR) results. In vitro cytotoxicity was also assessed using L929 cells, and the results demonstrated the material biocompatibility. The tunable properties, controlled release profiles, and biocompatibility of injectable poly(ethylene glycol) hydrogels support that they have great potential as a drug-releasing material for localized treatments.

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Section: Resultsmentioning

confidence: 85%

Injectable Poly(ethylene glycol) Hydrogels Cross-Linked by Metal–Phenolic Complex and Albumin for Controlled Drug Release

2020

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“…The positive control group was given doxorubicin (10 μg/mL), based on IC 50 value from previous research. 14 Medium was discarded after incubation and 150 μL of new medium containing 0.5 mg/mL MTT solution concentration in DMEM were added to each well. Then, the plate was incubated at 37°C for 4 hours until a formazan crystal formed.…”

Section: Methodsmentioning

confidence: 99%

Anticancer Activity of Methanol Extract of Ficus carica Leaves and Fruits Against Proliferation, Apoptosis, and Necrosis in Huh7it Cells

et al. 2019

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The polyphenol plant extracts have previously been demonstrated to act as chemopreventive and anticancer agents. Ficus carica is a rich source of polyphenols, yet its antioxidant and anticancer activities remain poorly characterized. This study aimed to determine the anticancer activity of F carica leaf and fruit extracts by investigating their impact on proliferation, apoptosis, and Huh7it cell necrosis. Leaves and fruits were extracted using methanol, and the phytochemical contents were analyzed using Fourier-transform infrared spectroscopy. The antioxidant activity was measured using the 2,2-diphenyl-1-picrylhydrazyl method. Anticancer activities were examined through MTT (3-(4,5-dimethylthiazol-2yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay on Huh7it liver cancer cells. The apoptosis and necrosis conditions were examined using Annexin biomarkers V-PI and later analyzed in flow cytometry. F carica leaves and fruit examined were found to have strong antioxidant activities with IC 50 values of 7.9875 µg/mL and 13.402 µg/mL, respectively. MTT assay results indicated F carica leaves and fruit had IC 50 values >653 μg/mL and >2000 μg/mL, respectively. The flow cytometry analysis indicated a higher percentage of Huh7it apoptosis and necrosis in leaf extracts compared with fruit extracts. The difference in anticancer activity was attributed to differing compounds present in each extract.

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“…The following classification of cells was made: cells with bright green nuclei were designated as healthy; cells with dense green nuclei (chromatin condensation) were considered as early apoptotic; cells with bright yellow nuclei and cytoplasm were termed late apoptotic; cells with orange nuclei and cytoplasm were classified as dead. Approximately 100 cells per replicate were analysed using cell counter plug-in of ImageJ software [22,33].…”

Section: Acridine Orange/ethidium Bromide Assay For Apoptosismentioning

confidence: 99%

Differential molecular and cellular responses of GLP-1 secreting L-cells and pancreatic alpha cells to glucotoxicity and lipotoxicity

Vasu

Moffett

McClenaghan

et al. 2015

Experimental Cell Research

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Cytotoxicity of α-terpineol in HeLa cell line and its effects to apoptosis and cell cycle

Cited by 13 publications

References 11 publications

Injectable Poly(ethylene glycol) Hydrogels Cross-Linked by Metal–Phenolic Complex and Albumin for Controlled Drug Release

Injectable Poly(ethylene glycol) Hydrogels Cross-Linked by Metal–Phenolic Complex and Albumin for Controlled Drug Release

Anticancer Activity of Methanol Extract of Ficus carica Leaves and Fruits Against Proliferation, Apoptosis, and Necrosis in Huh7it Cells

Differential molecular and cellular responses of GLP-1 secreting L-cells and pancreatic alpha cells to glucotoxicity and lipotoxicity

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