Cytotoxicity of three dentin bonding agents on human dental pulp cells

Chen, Ruey-Song; Liu, Ching-Cheng; Tseng, Wan-Yu; Jeng, Jiiang‐Huei; Lin, Chang‐Ching

doi:10.1016/s0300-5712(02)00088-x

Cited by 66 publications

(61 citation statements)

References 18 publications

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“…Materials were tested either diluted or undiluted without light curing. Several studies reported severe cell damage caused by undiluted materials [26,27], which decreased with increasing dilutions of the materials [27][28]. These findings were concordant with our study.…”

Section: Discussionsupporting

confidence: 93%

Testing endodontic sealers on human umbilical vein endothelial cells

et al. 2013

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AbstractThe toxic potential of the endodontic sealers ingredients, especially the unreacted monomer, that can irritate the periapical tissue and interfere with the healing process, thus having a negative impact on the biocompatibility of the material. The aim of this study was to evaluate the influence of three experimental endodontic sealers on cells viability in vitro. Human umbilical vein endothelial cells (HUVEC) were used. The experiments were done with solid samples and extracts of sealers in artificial saliva and water. The experiments evaluated the cytotoxicity of the residual monomers that resulted from the tested composites. The decrease in cell viability was quantified by colorimetric measurement of formazan. The components of the sealers dissolved in artificial saliva and water were determined by high performance liquid chromatography (HPLC). The HUVEC are a novelty for testing the endodontic sealers biocompatibility, with certain advantages compared to other cell types used in the literature, e.g. HELA cells, fibroblasts. The data showed that cytotoxicity was directly linked with the unreacted monomer — 2-hydroxyethyl methacrylate (HEMA) present in these composites. Two of the three formulations had little or no cytotoxic effect, which makes them suitable for further testing in order to be used in endodontic treatment.

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Section: Discussionsupporting

confidence: 93%

Testing endodontic sealers on human umbilical vein endothelial cells

et al. 2013

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“…Chen et al 29) also reported that the cytotoxic effects of different dilutions of bonding agents on pulpal cells were increased in higher concentrations after 24 h incubation period. It is clear that the reduction in cytotoxic effects of the materials by dilutions were the results of reduced concentrations of harmful components.…”

Section: Discussionmentioning

confidence: 95%

Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests

et al. 2011

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This study evaluated the cytotoxicity of self-etching primers/adhesives by direct contact and dentin barrier tests. The three two-step self-etching systems Clearfil SE Bond (CSE), Clearfil Protect Bond (CPB), Prime&Bond NT/NRC (PB) and one-step self-etching systems Reactmer Bond (RB), Clearfil Tri-S Bond (CTS), and Adper Prompt L-Pop (AP) were examined. In direct contact tests, L929 cells were cultured in the presence of diluted solutions (50, 20, 10, and 1%) of primer/conditioner of adhesive systems. For dentin barrier tests, each system was applied onto 0.5 or 1.5 mm thick human dentin assembled in a simple pulp chamber device and incubated for 24 h at 37°C to make the diffusive components contact the L929 cells placed at the bottom of the chamber. The cytotoxic effects were assessed by MTT assay. Cell culture without application of any primers/adhesives served as the control for both tests. One-way ANOVA and Tukey HSD tests were used for statistical analyses. The direct contact tests demonstrated that CSE and CPB were less toxic than the other materials at all dilutions. In the dentin barrier tests, toxic effects of materials were reduced with an increase in thickness of intervening dentin. CSE and CPB showed less cytotoxicity than the other adhesives (p<0.05) when applied to 0.5 mm-thick dentin, and CSE was the least toxic in the 1.5 mm-dentin group (p<0.05). Dentin thickness positively affected biocompatibility of the tested bonding systems. Two-step self-etching systems with HEMA-based primers were more biocompatible than other self-etching adhesives.

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“…In the present study, the cytotoxicity of four different one-step, self-etching dentin bonding agents were analyzed with MTT assay, DCF assay, 8-OHdG measurement and comet assay in L929 mouse fibroblast cell culture model. The cytotoxicity of the resin based materials are related with dilution rate 3,27,28) . The tested dentin bonding agents had a dose-dependent (1:1>1:10>1:20) cytotoxic effect on L929 cells (Fig.…”

Section: Discussionmentioning

confidence: 99%

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

Atalayın

Armağan

Konyalιoğlu

et al. 2015

Dental Materials Journal

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Bond were added to the medium using extract method. The cells were cultured with or without resveratrol (RES) addition. MTT, reactive oxygen species (ROS), DCF, Comet and 8-OHdG measurements were performed. The agents had a dose-dependent (1:1>1:10>1:20) cytotoxic effect. Considering 1:10 concentration; Group D at 1 h (p<0.01) and Group B and D at 24 h had the weakest cytotoxic effect (p<0.05). After RES addition, the highest cell viability was determined in Groups B+RES and D+RES at 1 h and in Groups A+RES and B+RES at 24 h (p<0.01). The dentin bonding agents induced ROS production and DNA damage regarding to their composition. However, RES addition decreased the indicated parameters.

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Cytotoxicity of three dentin bonding agents on human dental pulp cells

Cited by 66 publications

References 18 publications

Testing endodontic sealers on human umbilical vein endothelial cells

Testing endodontic sealers on human umbilical vein endothelial cells

Evaluation of cytotoxic effects of six self-etching adhesives with direct and indirect contact tests

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

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